LOCUS pAJM.1642 4143 bp ds-DNA circular 09-JAN-2018 DEFINITION . ACCESSION urn.local...1515502574182.32 VERSION KEYWORDS . SOURCE ORGANISM . COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers misc_feature 1..53 /note="Geneious type: terminator" /label="L3S3P21" promoter 73..298 /label="pCin" misc_feature 225..245 /note="Geneious type: operator" /label="CinO" promoter 246..251 /label="-35" promoter 269..274 /label="-10" misc_feature 299..373 /note="Geneious type: insulator" /label="RiboJ" misc_feature 382..393 /note="Geneious type: RBS" /label="B0064" misc_feature 400..1119 /note="Geneious type: CDS" /label="YFP" misc_feature 1120..1180 /note="Geneious type: terminator" /label="L3S2P21" misc_feature 1185..1238 /note="Geneious type: promoter" /label="pLacI" promoter 1203..1208 /label="-35" promoter 1226..1231 /label="-10" RBS 1239..1266 /label="cin1" CDS 1267..2022 /label="CinR-AM" misc_feature 1480..1482 /note="Geneious type: mutation" /label="S72C" misc_feature 1504..1506 /note="Geneious type: mutation" /label="V80I" misc_feature 1606..1608 /note="Geneious type: mutation" /label="G114D" misc_feature 1609..1611 /note="Geneious type: mutation" /label="G115D" misc_feature 1675..1677 /note="Geneious type: mutation" /label="R137H" misc_feature 1924..1926 /note="Geneious type: mutation" /label="K220R" misc_feature 1936..1938 /note="Geneious type: mutation" /label="A224T" misc_feature 2028..2282 /note="Geneious type: terminator" /label="induction operon terminator region" rep_origin 2320..3156 /label="more\stringent\p15A" rep_origin 2593 /label="origin" CDS complement(3228..4043) /label="Kanamycin\phosphotransferase" ORIGIN 1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac 61 gatcgttggc tgccctttgt gcgtccaaac ggacgcacgg cgctctaaag cgggtcgcga 121 tctttcagat tcgctcctcg cgctttcagt ctttgttttg gcgcatgtcg ttatcgcaaa 181 accgctgcac acttttgcgc gacatgctct gatccccctc atctgggggg gcctatctga 241 gggaatttcc gatccggctc gcctgaacca ttctgctttc cacgaacttg aaaacgctag 301 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa 361 taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa gggcgaggag 421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 541 atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac 601 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc 661 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 841 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 901 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 961 ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta ccagtccgcc 1021 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 1081 gccgggatca ctctcggcat ggacgagctg tacaagtaac tcggtaccaa attccagaaa 1141 agaggcctcc cgaaaggggg gccttttttc gttttggtcc aatggcggcg cgccatcgaa 1201 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg 1261 gtgaatatga ttgagaatac ctatagcgaa aagttcgagt ccgcgttcga acagatcaaa 1321 gcggcggcca acgtggatgc cgccatccgt attctccagg cggaatataa cctcgatttc 1381 gtcacctacc atctcgccca gacaatcgcg agcaagatcg attcgccctt cgtgcgcacc 1441 acctatccgg atgcctgggt ttcccgttac ctcctcaact gctatgtgaa ggtcgatccg 1501 atcatcaagc agggcttcga acgccagctg cccttcgact ggagcgaggt cgaaccgacg 1561 ccggaggcct atgccatgct ggtcgacgcc cagaaacacg gcatcgatga caatggctac 1621 tccatccccg tcgccgacaa ggcgcagcgc cgcgccctgc tgtcgctgaa tgcccatata 1681 ccggccgacg aatggaccga gctcgtgcgc cgctgccgca atgagtggat cgagatcgcc 1741 catctgatcc accgcaaggc cgtatatgag ctgcatggcg aaaacgatcc ggtgccggca 1801 ttgtcgccgc gcgagatcga gtgtctgcac tggaccgccc tcggcaagga ttacaaggat 1861 atttcggtca tcctgggcat atcagagcat accacacgcg attacctgaa aaccgcccgc 1921 ttcaggctcg gctgcaccac gatctcggcc gccgcgtcgc gggctgttca attgcgcatc 1981 atcaatccct ataggatccg catgacgcga cgtaattggt aatagcctaa ttggtaacga 2041 atcagacaat tgacggctcg agggagtagc atagggtttg cagaatccct gcttcgtcca 2101 tttgacaggc acattatgca tcgatgataa gctgtcaaac atgagcagat cctctacgcc 2161 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 2221 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcaa atattttatc 2281 tgaggtgctt cctcgctcac tgactcgctg cacgaggcag acctcagcgc tagcggagtg 2341 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg 2401 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc 2461 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa 2521 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg 2581 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct 2641 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg 2701 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat 2761 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag 2821 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat 2881 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat 2941 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag 3001 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag 3061 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg 3121 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga 3181 aaaatcaatc taaagtatat atgagtaaac ttggtctgac agttacctta gaaaaactca 3241 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga 3301 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga 3361 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc 3421 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag 3481 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg 3541 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga 3601 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc 3661 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc 3721 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg 3781 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc 3841 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca 3901 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc 3961 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac 4021 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt 4081 tttattgttc atgatgatat atttttatct tgtgcaatgt acatcagaga ttttgagaca 4141 caa //