LOCUS       Exported               10758 bp ds-DNA     circular SYN 28-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pScI_dCas9-CDA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Nishida
  TITLE     Direct Submission
  JOURNAL   Exported Mar 2, 2018 from SnapGene 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8017..8851
                     /organism="Gateway binary vector pGWB1"
                     /mol_type="other DNA"
                     /db_xref="taxon:419545"
     misc_feature    193..633
                     /label=repH
     primer_bind     697..716
                     /label=kn1008_repH-Erm_F
     primer_bind     903..968
                     /label=kn1006_SacI-pThl_F
     promoter        908..980
                     /label=pThl
     primer_bind     942..1011
                     /label=kn1005_pThl-ErmB_F
     CDS             987..1724
                     /codon_start=1
                     /label=ErmB
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     primer_bind     complement(1696..1750)
                     /label=kn1004_ErmB_NsiI_R
     primer_bind     1893..1916
                     /label=M13F
     primer_bind     complement(1917..1936)
                     /label=kn1009_M13FR
     primer_bind     1920..1936
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1937..1960
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1961..2024
                     /label=rrnBt
     primer_bind     1961..2001
                     /label=kn794_Sal-Spe-rrnBT
     primer_bind     complement(1984..2043)
                     /label=kn793_PmCDA1-rrnBT
     CDS             complement(2031..2654)
                     /codon_start=1
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     primer_bind     complement(2033..2052)
                     /label=kn872_PmCDA1end_F
     misc_feature    2205..2228
                     /label=flap
     primer_bind     2229..2252
                     /label=kn681_PmCDA1_flap_Stop_R
     misc_feature    2235
                     /label=SQ variant from 1256
     primer_bind     complement(2351..2370)
                     /label=kn711_PmCDA1-mid_F
     primer_bind     complement(2634..2654)
                     /label=kn680_PmCDA1_start_F
     primer_bind     complement(2636..2684)
                     /label=kn575_Flag-PmCDA1_F
     CDS             complement(2661..2726)
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(2745..2810)
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     2802..2839
                     /label=kn523_SH3-BspE1_R
     misc_feature    2817..2987
                     /label=SH3 noRe
     primer_bind     complement(2823..2852)
                     /label=kn805_SH3P49L_F
     primer_bind     2826..2857
                     /label=kn806_SH3P49L_R
     CDS             complement(2838..2840)
                     /codon_start=1
                     /label=P49L for SH3 disruption
                     /translation="P"
     primer_bind     complement(2880..2945)
                     /label=kn807_SH3_BglSap1KO_F
     primer_bind     2925..2993
                     /label=kn808_SH3_BglPstApa1KO_R
     primer_bind     complement(2994..3017)
                     /label=kn555_GS-link_F
     primer_bind     complement(2997..3037)
                     /label=kn790
     CDS             complement(3018..7116)
                     /codon_start=1
                     /label=dCas9
                     /translation="KKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNL
                     IGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFL
                     VEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR
                     GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLEN
                     LIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIG
                     DQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQ
                     LPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK
                     QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNS
                     RFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFT
                     VYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSV
                     EISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLK
                     TYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQL
                     IHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHK
                     PENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLY
                     YLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSE
                     EVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVA
                     QILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNA
                     VVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEI
                     TLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKES
                     ILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITI
                     MERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELA
                     LPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADAN
                     LDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDA
                     TLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(3059..3078)
                     /label=kn837_eCas9end_F
     primer_bind     3106..3129
                     /label=kn660eCas9_4k_R
     primer_bind     complement(3267..3288)
                     /label=kn661eCas9_3.8k_F
     primer_bind     3624..3646
                     /label=kn659eCas9_3.5k_R
     primer_bind     4130..4149
                     /label=kn655eCas9_3k_R
     primer_bind     complement(4519..4549)
                     /label=kn728_eCas9_N863A_F
     primer_bind     4520..4550
                     /label=kn729eCas9_N863A_R
     misc_feature    complement(4533..4535)
                     /label=N863A for nickase
     primer_bind     complement(4583..4619)
                     /label=kn650_eCas9_H840A_F
     primer_bind     4588..4621
                     /label=kn651_eCas9_H840A_R
     misc_difference 4602..4604
                     /label=H840A
     primer_bind     5510..5530
                     /label=kn658eCas9_1.5k_R
     primer_bind     complement(5665..5684)
                     /label=kn656eCas9_1.5k_F
     primer_bind     5830..5853
                     /label=kn779_eCas9_1.3k_R
     primer_bind     6633..6653
                     /label=kn654eCas9_500R
     primer_bind     complement(6742..6764)
                     /label=kn657eCas9_500_F
     primer_bind     7036..7057
                     /label=kn798_eCas9_100R
     primer_bind     complement(7074..7109)
                     /label=kn652eCas9_D10A_F
     primer_bind     7077..7113
                     /label=kn653eCas9_D10A_R
     misc_difference 7092..7094
                     /label=D10A
     primer_bind     7113..7162
                     /label=kn951_eCas9-OR1_R
     primer_bind     7115..7136
                     /label=kn817_chimera_autostop_F
     primer_bind     complement(7116..7136)
                     /label=kn816_chimera_autostop_R
     misc_feature    7117..7136
                     /label=autostop target
     CDS             complement(7117..7121)
                     /codon_start=1
                     /label=dCas9
                     /translation="M"
     primer_bind     7136..7197
                     /label=kn1256_HiRepOR_F
     primer_bind     7137..7206
                     /label=kn1026_Lambda-SD_ex_F
     misc_feature    7144
                     /label=ts
     -10_signal      complement(7151..7157)
                     /label=-10
     misc_feature    7153..7169
                     /label=OR1
     misc_feature    7177..7193
                     /label=OR2
     primer_bind     complement(7180..7230)
                     /label=kn1165_OR2-HighTS_R
     primer_bind     7190..7259
                     /label=kn1025_lambda-SD_F
     misc_feature    7200..7216
                     /label=OR3
     misc_feature    7227..7239
                     /label=SD
     CDS             7240..7953
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=-lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAEMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     primer_bind     complement(7258..7277)
                     /label=kn936_cI-start_R
     primer_bind     complement(7562..7588)
                     /label=kn956_cI_G113E_R
     primer_bind     7563..7589
                     /label=kn957_cI_G113E_F
     CDS             7576..7578
                     /label=G to E for RecA resistant
     primer_bind     7930..7949
                     /label=kn937_cI-end_F
     primer_bind     complement(7989..8011)
                     /label=kn954_OL-cI_R
     misc_feature    8011
                     /label=Scar
                     /note="Left operator"
     primer_bind     8012..8040
                     /label=kn992_Cm-Xba1_R
     misc_feature    8017..8851
                     /label=CmUnit
     CDS             complement(8082..8741)
                     /codon_start=1
                     /transl_table=11
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /db_xref="GI:122703310"
                     /protein_id="BAF44947.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(8082..8741)
                     /gene="cat"
                     /label=cat
     promoter        complement(8795..8825)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(8821..8843)
                     /label=kn570_T7t-Nsi1-LacP_F
     primer_bind     complement(8834..8880)
                     /label=kn993_Cm-Age1_F
     primer_bind     8855..8897
                     /label=kn955_gRNA-Rep101_R
     primer_bind     complement(8884..8906)
                     /label=kn1263_2xgRNA-CM_R
     CDS             complement(8989..9939)
                     /codon_start=1
                     /gene="rep101"
                     /product="protein needed for replication with the pSC101 
                     origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTMKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     8989..9010
                     /label=kn933_rep101end_R
     primer_bind     complement(9053..9074)
                     /label=kn934_rep101end_F
     misc_feature    9170
                     /label=mut SQ in 1702
     misc_feature    9773
                     /label=mut in SQ 1702
     primer_bind     complement(9794..9821)
                     /label=kn1028_Rep101_M44R_F
     primer_bind     9798..9825
                     /label=kn1027_Rep101_M44R_R
     misc_structure  complement(9811..9813)
                     /label=Mutation to M in 1702 1703
     primer_bind     9977..9999
                     /label=kn931_pSC101_ORI_R
     rep_origin      9987..10209
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(10182..10201)
                     /label=kn932_pSC101_ORI_F
     primer_bind     10196..10254
                     /label=kn1154_pKNT19_aat2_F
     primer_bind     complement(10207..10232)
                     /label=kn952_ER-pSC101_F
     promoter        complement(10233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10689..10734
                     /label=kn788_pKNT19_Aat2-Sac2
     primer_bind     complement(10715..10734)
                     /label=kn1155_pKNT19_aat2_R
ORIGIN
        1 cgacgcttta tatagaaaag aagattcaac taggtaaaat cttaatatag gttgagatga
       61 taaggtttat aaggaatttg tttgttctaa tttttcactc attttgttct aatttctttt
      121 aacaaatgtt cttttttttt tagaacagtt atgatatagt tagaatagtt taaaataagg
      181 agtgagaaaa agatgaaaga aagatatgga acagtctata aaggctctca gaggctcata
      241 gacgaagaaa gtggagaagt catagaggta gacaagttat accgtaaaca aacgtctggt
      301 aacttcgtaa aggcatatat agtgcaatta ataagtatgt tagatatgat tggcggaaaa
      361 aaacttaaaa tcgttaacta tatcctagat aatgtccact taagtaacaa tacaatgata
      421 gctacaacaa gagaaatagc aaaagctaca ggaacaagtc tacaaacagt aataacaaca
      481 cttaaaatct tagaagaagg aaatattata aaaagaaaaa ctggagtatt aatgttaaac
      541 cctgaactac taatgagagg cgacgaccaa aaacaaaaat acctcttact cgaatttggg
      601 aactttgagc aagaggcaaa tgaaatagat tgacctccca ataacaccac gtagttattg
      661 ggaggtcaat ctatgaaatg cgattaagct ttttctaatt cacataagcg tgcaggttta
      721 aagtacataa aaaatataat gaaaaaaagc atcattatac taacgttata ccaacattat
      781 actctcatta tactaattgc ttattccaat ttcctattgg ttggaaccaa caggcgttag
      841 tgtgttgttg agttggtact ttcatgggat taatcccatg aaacccccaa ccaactcgcc
      901 aaagctctat attgataaaa ataataatag tgggtataat taagttgtta gagaaaacgt
      961 ataaattagg agggattcat gctggtatga acaaaaatat aaaatattct caaaactttt
     1021 taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata
     1081 ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta
     1141 aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa
     1201 aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca
     1261 aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa
     1321 aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca
     1381 agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc
     1441 agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct
     1501 taataaaact tacccgccat accacagatg ttccagataa atattggaag ctatatacgt
     1561 actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc
     1621 atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat
     1681 tgtctatttt taatagttat ctattattta acgggaggaa ataaatgcat aaactgcatc
     1741 ccttaacttg tttttcgtgt acctattttt tgtgaatcgc cattcgccat tcaggctgcg
     1801 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1861 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1921 taaaacgacg gccagtgaat tcgagctcgg tacccggccg caaacaacag ataaaacgaa
     1981 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctgtcaagt aacagcagga
     2041 ctcttagtgg tgtggagtat ttttacctga atcataatgg acaactcgct ccgtcgtttt
     2101 tcagctcgct tcaaagtctt ctcaagccat ctattctcat tcaattgatt gtgcgacgat
     2161 tggatgaata ttttcctgca acattggtag tgttcactta ccattacatt caacccaacc
     2221 ccgttatctc tgagattcca cagcccaatt tgattcctcg catttttctc gtaatagagt
     2281 ttgcaagccc agattttcaa agtgtggccg ttcccccgca gctcctggtt ataccattct
     2341 aagatctttt cagcgcaatc tgcacaagga ctccaggatg agtaccaatt tatcgtgaat
     2401 tgtccggggt tgtcgcgcag gtattcttcg acttttctaa tgctaaagat ttcggcgtga
     2461 atgccacgtt ctgtcccgct ctgtggttta ttcacagcat agccccaaaa acacgctcta
     2521 cgttcacccc gtcgttttaa ttcaaagaga acgtagcatc tatgcgacac ggattttttg
     2581 ttgttgaaaa actgtttctt aaacgtgtag atgtccaact tctcatggat tctcacgtac
     2641 tcagcgtcgg tcatcctaga cttatcgtca tcgtctttgt aatcaatatc atgatccttg
     2701 tagtctccgt cgtggtcctt atagtctccg gactcgagcc tagacttatc gtcatcgtct
     2761 ttgtaatcaa tatcatgatc cttgtagtct ccgtcgtggt ccttatagtc tccggaatac
     2821 ttctccacgt aagggacagg aatcatcccc ctctttcctt cgctgtcctc tgcattccac
     2881 cactgctcct caggcttatc ccggattctc aggatgtctc ctttcttaaa gggaagatcc
     2941 tcttcatcat tcccattaaa gtcaaagagg gctcgcacat actcagcaga acctccacct
     3001 ccagaacctc ctccaccgtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga
     3061 ccagtgatgg attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt
     3121 ttacgatcaa ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac
     3181 gtaaataaat gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta
     3241 tatgcactaa gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca
     3301 ctgatttgct caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt
     3361 tgttcgttat cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa
     3421 ttcacatatt tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc
     3481 agcatccgtt tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt
     3541 aagtcttttt taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca
     3601 aaggaacttc tttccataat tgtgatccct agtaactctt taacggattt taacttcttc
     3661 gatttccctt tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca
     3721 aaaccaccat atttttttgg atcccagtct tttttacgag caataagctt gtccgaattt
     3781 ctttttggta aaattgactc cttggagaat ccgcctgtct gtacttctgt tttcttgaca
     3841 atattgactt ggggcatgga caatactttg cgcactgtgg caaaatctcg ccctttatcc
     3901 cagacaattt ctccagtttc cccattagtt tcgattagag ggcgtttgcg aatctctcca
     3961 tttgcaagtg taatttctgt tttgaagaag ttcatgatat tagagtaaaa gaaatatttt
     4021 gcggttgctt tgcctatttc ttgctcagac ttagcaatca ttttacgaac atcataaact
     4081 ttataatcac catagacaaa ctccgattca agttttggat atttcttaat caaagcagtt
     4141 ccaacgacgg catttagata cgcatcatgg gcatgatggt aattgttaat ctcacgtact
     4201 ttatagaatt ggaaatcttt tcggaagtca gaaactaatt tagattttaa ggtaatcact
     4261 ttaacctctc gaataagttt atcattttca tcgtatttag tattcatgcg actatccaaa
     4321 atttgtgcca catgcttagt gatttggcga gtttcaacca attggcgttt gataaaacca
     4381 gctttatcaa gttcactcaa acctccacgt tcagctttcg ttaaattatc aaacttacgt
     4441 tgagtgatta acttggcgtt tagaagttgt ctccaatagt ttttcatctt tttgactact
     4501 tcttcacttg gaacgttatc cgatttacca cgatttttat cagaacgcgt taagacctta
     4561 ttgtctattg aatcgtcttt aaggaaactt tgtggaacaa tggcatcgac atcataatca
     4621 cttaaacgat taatatctaa ttcttggtcc acatacatgt ctcttccatt ttggagataa
     4681 tagagataga gcttttcatt ttgcaattga gtattttcaa caggatgctc tttaagaatc
     4741 tgacttccta attctttgat accttcttcg attcgtttca tacgctctcg cgaatttttc
     4801 tggccctttt gagttgtctg attttcacgt gccatttcaa taacgatatt ttctggctta
     4861 tgccgcccca ttactttgac caattcatca acaactttta cagtctgtaa aatacctttt
     4921 ttaatagcag ggctaccagc taaatttgca atatgttcat gtaaactatc gccttgtcca
     4981 gacacttgtg ctttttgaat gtcttcttta aatgtcaaac tatcatcatg gatcagctgc
     5041 ataaaattgc gattggcaaa accatctgat ttcaaaaaat ctaatattgt tttgccagat
     5101 tgcttatccc taataccatt aatcaatttt cgagacaaac gtccccaacc agtataacgg
     5161 cgacgtttaa gctgtttcat caccttatca tcaaagaggt gagcatatgt tttaagtctt
     5221 tcctcaatca tctccctatc ttcaaataag gtcaatgtta aaacaatatc ctctaagata
     5281 tcttcatttt cttcattatc caaaaaatct ttatctttaa taatttttag caaatcatgg
     5341 taggtaccta atgaagcatt aaatctatct tcaactcctg aaatttcaac actatcaaaa
     5401 cattctattt ttttgaaata atcttctttt aattgcttaa cggttacttt tcgatttgtt
     5461 ttgaagagta aatcaacaat ggctttcttc tgttcacctg aaagaaatgc tggttttcgc
     5521 attccttcag taacatattt gacctttgtc aattcgttat aaaccgtaaa atactcataa
     5581 agcaaactat gttttggtag tactttttca tttggaagat ttttatcaaa gtttgtcatg
     5641 cgttcaataa atgattgagc tgaagcacct ttatcgacaa cttcttcaaa attccatggg
     5701 gtaattgttt cttcagactt ccgagtcatc catgcaaaac gactattgcc acgcgccaat
     5761 ggaccaacat aataaggaat tcgaaaagtc aagatttttt caatcttctc acgattgtct
     5821 tttaaaaatg gataaaagtc ttcttgtctt ctcaaaatag catgcagctc acccaagtga
     5881 atttgatggg gaatagagcc gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga
     5941 tttagtttca ccaataattc ctcagtacca tccatttttt ctaaaattgg tttgataaat
     6001 ttataaaatt cttcttggct agctccccca tcaatataac ctgcatatcc gttttttgat
     6061 tgatcaaaaa agatttcttt atacttttct ggaagttgtt gtcgaactaa agcttttaaa
     6121 agagtcaagt cttgatgatg ttcatcgtag cgtttaatca ttgaagctga taggggagcc
     6181 ttagttattt cagtatttac tcttaggata tctgaaagta aaatagcatc tgataaattc
     6241 ttagctgcca aaaacaaatc agcatattga tctccaattt gcgccaataa attatctaaa
     6301 tcatcatcgt aagtatcttt tgaaagctgt aatttagcat cttctgccaa atcaaaattt
     6361 gatttaaaat taggggtcaa acccaatgac aaagcaatga gattcccaaa taagccattt
     6421 ttcttctcac cggggagctg agcaatgaga ttttctaatc gtcttgattt actcaatcgt
     6481 gcagaaagaa tcgctttagc atctactcca cttgcgttaa tagggttttc ttcaaataat
     6541 tgattgtagg tttgtaccaa ctggataaat agtttgtcca catcactatt atcaggattt
     6601 aaatctccct caatcaaaaa atgaccacga aacttaatca tatgcgctaa ggccaaatag
     6661 attaagcgca aatccgcttt atcagtagaa tctaccaatt tttttcgcag atgatagata
     6721 gttggatatt tctcatgata agcaacttca tctactatat ttccaaaaat aggatgacgt
     6781 tcatgcttct tgtcttcttc caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca
     6841 tctactttcg ccatctcatt tgaaaaaatc tcctgtagat aacaaatacg attcttccga
     6901 cgtgtatacc ttctacgagc tgtccgtttg agacgagtcg cttccgctgt ctctccactg
     6961 tcaaataaaa gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga
     7021 accttgaact ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta
     7081 tttgtgccga tagctaagcc tattgagtat ttcttatcca tttttgcctc ctaaaatggg
     7141 ccctgcaacc attatcaccg ccagaggtaa aatagtcaac acgcgcggtg ttagatattt
     7201 atcccttgcg gtgatagatt taacgttaag gaggtatgta tgagcacaaa aaagaaacca
     7261 ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa
     7321 aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc
     7381 gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttaca
     7441 aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag
     7501 atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt
     7561 tctcatgttc aggcagagat gttctcacct aagcttagaa cctttaccaa aggtgatgcg
     7621 gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa
     7681 ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt
     7741 ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt
     7801 gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta
     7861 aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc
     7921 gctagtcagt ggcctgaaga gacgtttggc tgatcggcaa ggtgttctgg tcggcgcata
     7981 gctgataaca attgagcaag aatcttcatc gctagaccgc aaaaatcagc gcgcaaatac
     8041 gcatactgtt atctggcttt tagtaagccg gatccacgcg tttacgcccc gccctgccac
     8101 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg
     8161 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     8221 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     8281 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     8341 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     8401 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     8461 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     8521 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     8581 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     8641 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     8701 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     8761 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt
     8821 gtaaagcctg gggtgcctaa tgcggcccct ctagctaccg gttttggtca tgagattatc
     8881 tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg
     8941 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc
     9001 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga
     9061 accattgaga tcatgcttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag
     9121 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga
     9181 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag
     9241 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg
     9301 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat
     9361 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa
     9421 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt
     9481 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac
     9541 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc
     9601 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat
     9661 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt
     9721 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gaacgtattg
     9781 gttataagtg aacgataccg tccgttcttt cattgtaggg ttttcaatcg tggggttgag
     9841 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat
     9901 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg
     9961 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct
    10021 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc
    10081 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt
    10141 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt
    10201 gtataactca ctactttagt cagttccgac gtgcacattt ccccgaaaag tgccacctga
    10261 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10321 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10441 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10561 caggcgatta tgtcttttgc gcattcactt cttttctata taaatatgag cgaagcgaat
    10621 aagcgtcgga aaagcagcaa aaagtttcct ttttgctgtt ggagcatggg ggttcagggg
    10681 gtgcagtatc tgacgtcaat gccgagcgaa agcgagccga agggtagcat ttacgttaga
    10741 taaccccctg atatgctc
//
