LOCUS Exported 12774 bp ds-DNA circular SYN 02-MAR-2018 DEFINITION plasmid HIV Alex George Enhanced. ACCESSION Your VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12774) AUTHORS Self JOURNAL Unpublished. REFERENCE 2 (bases 1 to 12774) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 2, 2018 from SnapGene 4.1.6 http://www.snapgene.com COMMENT HRST Ligated into PUC WPRE fixed Changed cPPT to include SpeI site for cloning promoters cloned in clean CMV promoter without extraneous sequence 9/19/03 Completely sequenced with 4x coverage to confirm perfect sequence 10/08/03 Mutations found in IRES Element Between BamHI and NdeI sites 11/03/03 After Re-cloning the IRES from TOPO vector sequence is now confirmed to be perfect (IRES contains 7As at one point which seems to only be 6As in MCV genome) 11/13/03 Confirmed shortened cloning to add SmaI/PacI and tag 1/27/04 Confirmed perfect second position ZsGreen sequence with multiple oligos 5/24/04 Sequencing confirmed perfect cPPT-CMV-DsRed Express region ORIGDB|GenBank FEATURES Location/Qualifiers source 1..12774 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..636 /gene="LTR" /label=LTR /note="Long Terminal Repeat" /note="color: #66cc99; direction: RIGHT" misc_feature 1..453 /label=HIV U3 /note="Full Length HIV U3" /note="color: #66cc99; direction: RIGHT" misc_feature 454..551 /label=HIV R /note="HIV Repeat Region" /note="color: #3366cc" misc_feature 552..636 /label=HIV U5 /note="HIV U5" /note="color: #3366cc" misc_feature 636..658 /label=PBS-K /note="tRNA binding site for Lysine tRNA" /note="color: #3366cc" misc_feature 637..1155 /gene="psi" /product="pbs-gag" /label=PSI /note="PSI Packaging Sequence" /note="color: #66cc99; direction: RIGHT" misc_feature 697..806 /label=HIV-PSI /note="color: #66cc99; direction: RIGHT" misc_feature 807..1144 /label=HR-ePSI /note="color: #66cc99; direction: RIGHT" misc_feature 1303..1536 /gene="rre" /product="minimal RRE" /label=RRE /note="Rev Responsive Element" /note="color: #66cc99; direction: RIGHT" primer_bind 1431..1449 /label=RRE seq for /note="color: black; sequence: ctattgaggcgcaacagca" misc_feature 2028..2033 /label=(EcoRI/MfeI) /note="color: #3366cc" misc_feature 2034..2211 /gene="cppt" /label=HIV cpPu (Trip) /note="Central Poly Purine Track" /note="color: #66cc99; direction: RIGHT" misc_feature 2299..2602 /label=CMV enhancer /note="color: #ffffff" misc_feature 2603..2806 /label=CMV promoter /note="color: #ffffff" primer_bind 2757..2777 /label=5' sequencing primer (CMV promoter) /note="color: black; sequence: cgcaaatgggcggtaggcgtg" misc_feature 2808..2826 /label=tet operator /note="color: #008080" misc_feature 2829..2847 /label=tet operator /note="color: #008080" CDS 2977..2997 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /note="color: #993366" /translation="PKKKRKV" CDS 3004..3051 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /note="color: #993366" /translation="KRPAATKKAGQAKKKK" CDS 3064..3384 /codon_start=1 /product="destabilization domain that can be stabilized by Shield1 in the ProteoTuner(TM) system" /label=DD /note="L106P mutant of FKBP12" /note="color: #993366" /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL KPE" misc_feature 3403..7515 /label=dCas9 /note="color: #66cc99; direction: RIGHT" primer_bind 7294..7313 /label=C-SpyCas9_for /note="color: black; sequence: cacagggataagcccatcag" misc_feature 7528..7548 /label=NLS /note="color: #ff99cc" misc_feature 7561..8268 /label=mCherry /note="color: #66cc99; direction: RIGHT" primer_bind complement(7816..7835) /label=mCherry_rev /note="color: black; sequence: aaggacagcttcaagtagtc" primer_bind 8054..8073 /label=mCherry_for /note="color: black; sequence: agatcaagcagaggctgaag" misc_feature 8296..8319 /label=Flag Tag /note="color: #00ff00" primer_bind complement(8296..8316) /label=FLAG_rev #809 /note="color: black; sequence: atcgtcgtcatccttgtagtc" misc_feature 8320..9066 /label=APEX2 protein /note="color: #ff0000" primer_bind 8404..8423 /label=APEX2_for #813 /note="color: black; sequence: AAGAGATGCGCTCCTCTAAT" primer_bind complement(8445..8464) /label=APEX2_rev #814 /note="color: black; sequence: CCTTGTCAAAGGTTCCAGCA" CDS 9073..9093 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /note="color: #993366" /translation="PKKKRKV" CDS 9100..9147 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /note="color: #993366" /translation="KRPAATKKAGQAKKKK" misc_feature 9163..9754 /label=WPRE /note="color: #66cc99; direction: RIGHT" primer_bind complement(9309..9328) /label=3' sequencing primer (WPRE_rev #815) /note="color: black; sequence: agacagcaaccaggatttat" misc_feature 9760..9848 /label=HIV nef /note="HIV nef" /note="color: #3366cc" misc_feature 9825..10060 /gene="dU3 LTR" /label=dU3 LTR /note="Deleted U3 Long-Terminal Repeat" /note="color: #66cc99; direction: RIGHT" misc_feature 9862..9977 /label=HIV R /note="HIV Repeat Region" /note="color: #66cc99; direction: RIGHT" modified_base 9873 /label=Difference from 5' LTR /note="This T is a C in the 5' LTR" /note="color: #000000" misc_feature 9978..10060 /label=HIV U5 /note="HIV U5" /note="color: #3366cc" misc_feature 10061..10131 /label=3' Flank /note="Chromosomal 3' Flanking Sequence carried over from original HIV integration site" /note="color: #3366cc" misc_feature 10271..10369 /label=Bla promoter /note="color: #3366cc" CDS 10370..11230 /codon_start=1 /gene="AMPr" /product="beta-lactamase (mature form)" /label=AMPr /note="Ampicillin Resistance Gene" /note="color: #ff9966" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10522..10541 /label=Amp resis seq for /note="color: black; sequence: cggtaagatccttgagagtt" primer_bind 11423..11442 /label=pUC Ori seq for /note="color: black; sequence: gtaatctgctgcttgcaaac" rep_origin 11456..11989 /direction=RIGHT /gene="ORI" /label=ORI /note="Bacterial Origin of Replication" /note="color: #ff9966" misc_feature 12226..12349 /gene="SV40 ori" /label=SV40 ori /note="color: #66cc99; direction: RIGHT" primer_bind 12395..12416 /label=Misc seq primer /note="color: black; sequence: gcaattaatgtgagttagctca" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat 2221 gctagctgat gcgggcccta gttattaata gtaatcaatt acggggtcat tagttcatag 2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga 2821 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat 2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag 2941 cctaccggtt aaggtgaacg cgtggccacc atggccccta aaaagaaacg caaggtcgaa 3001 gataaacgcc cagcagctac aaagaaggca ggtcaagcca agaaaaagaa ggaagatgca 3061 tgcggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc 3121 cagacctgtg tggtgcacta caccgggatg cttgaagatg gaaagaaagt cgattcctcc 3181 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg 3241 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat 3301 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc 3361 gatgtggagc ttctaaaacc ggaaggcagt gaattcggaa gcggaagcga caagaagtac 3421 tccattgggc tcgctatcgg taccaacagc gtcggctggg ccgtcattac ggacgagtac 3481 aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag 3541 aacctcattg gagccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa 3601 agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc 3661 tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt 3721 ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag 3781 gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct ggtagacagt 3841 actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcacatgat caaatttcgg 3901 ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt 3961 atccaactgg ttcagactta caatcagctt ttcgaggaga acccgatcaa cgcatccggc 4021 gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc 4081 atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca 4141 ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagctgcaa 4201 ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag 4261 tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt 4321 ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat 4381 gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag 4441 aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc 4501 ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc 4561 accgaggagc tgctggtaaa gctgaacaga gaagatctgt tgcgcaaaca gcgcactttc 4621 gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat cctcaggcgg 4681 caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca 4741 tttcggatac cctactatgt aggccccctc gctcggggaa attccagatt cgcgtggatg 4801 actcgcaaat cagaagagac catcactccc tggaacttcg aggaagtcgt ggataagggg 4861 gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa 4921 aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc 4981 aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag 5041 aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa 5101 gaagactatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat 5161 cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac 5221 ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg 5281 tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac 5341 aaagtcatga aacagctcaa gagacgccga tatacaggat gggggcggct gtcaagaaaa 5401 ctgatcaatg gcatccgaga caagcagagt ggaaagacaa tcctggattt tcttaagtcc 5461 gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag 5521 gaggacatcc agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct 5581 aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat 5641 gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga 5701 gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa 5761 gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag 5821 cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat 5881 caggaactgg acatcaaccg gttgtccgac tacgacgtgg atgctatcgt gccccaaagc 5941 tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg 6001 aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag 6061 ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga 6121 ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc 6181 cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa 6241 aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat 6301 ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat 6361 gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagctggaa 6421 tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct 6481 gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt 6541 ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca 6601 aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccgcaag 6661 gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc 6721 tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat 6781 tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt 6841 gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc 6901 atcacaatca tggagcgatc cagcttcgag aaaaacccca tcgactttct cgaagcgaaa 6961 ggatataaag aggtcaaaaa agacctcatc attaagctgc ccaagtactc tctctttgag 7021 cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag 7081 ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc 7141 aaagggtctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac 7201 cttgatgaga tcatcgagca aataagcgag ttctccaaaa gagtgatcct cgccgacgct 7261 aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag 7321 gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag 7381 tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc 7441 acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc 7501 ggtggagacg gctctactag tggctctccc aagaagaaga ggaaggtagg atccggaagt 7561 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 7621 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 7681 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 7741 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 7801 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 7861 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc actgcaggac 7921 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 7981 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 8041 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 8101 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc 8161 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 8221 cgcgctgagg gccgccactc caccggcggc atggacgagc tgtacaagtc tggcggccgc 8281 ggcggtggag gtagcgacta caaggatgac gacgataagg gaaagtctta cccaactgtg 8341 agtgctgatt accaggacgc cgttgagaag gcgaagaaga agctcagagg cttcatcgct 8401 gagaagagat gcgctcctct aatgctccgt ttggcattcc actctgctgg aacctttgac 8461 aagggcacga agaccggtgg acccttcgga accatcaagc accctgccga actggctcac 8521 agcgctaaca acggtcttga catcgctgtt aggcttttgg agccactcaa ggcggagttc 8581 cctattttga gctacgccga tttctaccag ttggctggcg ttgttgccgt tgaggtcacg 8641 ggtggaccta aggttccatt ccaccctgga agagaggaca agcctgagcc accaccagag 8701 ggtcgcttgc ccgatcccac taagggttct gaccatttga gagatgtgtt tggcaaagct 8761 atggggctta ctgaccaaga tatcgttgct ctatctgggg gtcacactat tggagctgca 8821 cacaaggagc gttctggatt tgagggtccc tggacctcta atcctcttat tttcgacaac 8881 tcatacttca cggagttgtt gagtggtgag aaggaaggtc tccttcagct accttctgac 8941 aaggctcttt tgtctgaccc tgtattccgc cctctcgttg acaaatatgc agcggacgaa 9001 gatgccttct ttgctgatta cgctgaggct caccaaaagc tttccgagct tgggtttgct 9061 gatgccctcg agcctaaaaa gaaacgcaag gtcgaagata aacgcccagc agctacaaag 9121 aaggcaggtc aagccaagaa aaagaagggc tcttaatcta gataatcaac ctctggatta 9181 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 9241 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 9301 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 9361 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 9421 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 9481 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 9541 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg 9601 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 9661 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 9721 gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac 9781 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 9841 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 9901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 9961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 10021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 10081 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta 10141 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt 10201 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 10261 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 10321 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 10381 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 10441 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 10501 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 10561 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc 10621 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 10681 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 10741 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 10801 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 10861 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 10921 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 10981 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 11041 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 11101 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 11161 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 11221 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 11281 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 11341 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 11401 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 11461 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 11521 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 11581 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 11641 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 11701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 11761 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 11821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 11881 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 11941 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 12001 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 12061 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 12121 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 12181 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga 12241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 12301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag 12361 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 12421 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 12481 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc 12541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 12601 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc 12661 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt 12721 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //