LOCUS       BB3nK_ext_AC      2642 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     Vector          1..678
                     /origin="pSTBLUE-1 wo_ORI_circular_pUC_ORI_aus_pGAPZB
                     (2.745bp circular)Target vector: from BbsI cut at
                     2054^2055 to BbsI cut at 2732^2733, 678bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     Vector          join(777..2642,1..722)
                     /origin="pUCori_Kan_NTS_Linker_TT_FsA_BamXho_FsC (2,618bp
                     circular)Target vector: from BsaI cut at 752^753 to BsaI
                     cut at 722^723, 2,588bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
     oriT            complement(5..678)
                     /label="pUC_ORI_aus_pGAPZB"
     misc_feature    join(<777..780,679..>722)
                     /feature_type="Insertion"
                     /origin="FS1_TT_FsA_BamXho_FsC_FS2 (106bp
                     linear)Fragment: from BbsI cut at 12^13 to BbsI cut at
                     90^91, 78bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     terminator      683..722
                     /label="BBa_B1007"
     misc_feature    723..776
                     /feature_type="Insertion"
                     /origin="BB3_pTEF_KanR_TEFt_AC_BpiI(extern Linker) (78bp
                     linear)Fragment: from BsaI cut at 10^11 to BsaI cut at
                     64^65, 54bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     primer          723..776
                     /label="FS_AC_BpiI(extern)_fw"
     primer          complement(727..780)
                     /label="FS_AC_BpiI(extern)_rev"
     Fragment        <736..>776
                     /origin="FS1FsA_BX_FsDFS2_FW (106bp linear)Fragment: from
                     BpiI cut at 12^13 to BpiI cut at 90^91, 78bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     misc_feature    781..2141
                     /feature_type="Insertion"
                     /origin="KanMX4_wo_BspMI (1.381bp linear)Fragment: from
                     BbsI cut at 8^9 to BbsI cut at 1369^1370,
                     1.361bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             785..1128
                     /label="TEFpromoter"
     misc_feature    958
                     /label="C to G"
     CDS             1129..1938
                     /label="KanR"
     CDS             1947..2141
                     /label="TEFterminator"
     misc_feature    2142..2642
                     /feature_type="Insertion"
                     /origin="NTS_frompGAPZB 500bp_SpeI (521bp
                     linear)Fragment: from BbsI cut at 8^9 to BbsI cut at
                     509^510, 501bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     CDS             <2146..2642
                     /label="NTS1_5s_NTS2"
ORIGIN
        1 CGCTGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
       61 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
      121 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
      181 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
      241 GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
      301 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
      361 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
      421 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
      481 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
      541 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
      601 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
      661 TGATCTTTTC TACGGGGTGG AGcgcaaaaa accccgcccc tgacagggcg gggttttttc
      721 gcGATCGAAG ACcCAATTGg agaccCTCGA GGGATCCGGT CTCCGATCGG GTCTTCAATT
      781 CATGGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC
      841 ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC
      901 CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACGTG
      961 CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA
     1021 GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA
     1081 AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT AAACAACCAT GGGTAAGGAA
     1141 AAGACTCACG TTTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT
     1201 AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT GTATGGGAAG
     1261 CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA
     1321 GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT
     1381 TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG CAAAACAGCA
     1441 TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG
     1501 TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA
     1561 TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT
     1621 GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAGCTTTTG
     1681 CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT
     1741 GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC
     1801 CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG
     1861 CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG
     1921 CTCGATGAGT TTTTCTAATC AGTACTGACA ATAAAAAGAT TCTTGTTTTC AAGAACTTGT
     1981 CATTTGTATA GTTTTTTTAT ATTGTAGTTG TTCTATTTTA ATCAAATGTT AGCGTGATTT
     2041 ATATTTTTTT TCGCCTCGAC ATCATCTGCC CAGATGCGAA GTTAAGTGCG CAGAAAGTAA
     2101 TATCATGCGT CAATCGTATG TGAATGCTGG TCGCTATACT GGCTTGTAAG CAATCTGGAC
     2161 AATTATGTAA GCGGGTTACG TAAACAGTTA TGTAAGCAGA AAAATTTCAA ACGACAAAAC
     2221 TTGGGGTCTA CAGACACAGT AGCCAGAAGA TTGCACTACC ATTCGACTCC TCATGACCCA
     2281 CTCTTTCGAT CCATGTAGTT AGGTTACCGT TTTTCCTAAT ATTTAAGGAT GTTGAAAATT
     2341 CATTTTCATT TTTTTCGTTT TTAAGATTTT CTCACAACTC TTCCAAAGAT TACTAGTTGA
     2401 CTTTTCAAAT ATTTAGGGTA TTTTTCTCAC TTTTTCCTAG CAAACTCCAA TTGGTGGGTT
     2461 CAGTGCAATG GAGTATCACC TTGCAACCAC AACGTAATAG CTAACTTGTG GCCACCATGT
     2521 CTGGTTGTAG AGATAATTGG ATTCTAATGT GGATCACATG ACTACTCACG TGTCAAAAAC
     2581 CCAACCTGAC TTGGCCCAGC TTAGCAAGAA TATTTCGAAT CCACTCTTGT GGCCTAGTGG
     2641 AC
//
