LOCUS       Exported                7155 bp ds-DNA     circular SYN 05-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2018 from SnapGene 4.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..2629
                     /label=source
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=pYPQ230 (LbCpf1)"
     source          2578..2629
                     /label=source(2)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=LbCpf1"
     source          2578..2627
                     /label=source(3)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=AsCpf1"
     source          6527..7155
                     /label=source(4)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=pYPQ230 (LbCpf1)"
     source          6527..6597
                     /label=source(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=source
                     ApEinfo_revcolor=pink
                     dnas_title=AsCpf1"
     promoter        1..138
                     /label=Spn promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=Spn promoter
                     vntifkey=30"
     CDS             139..1149
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=SpnR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=SpnR
                     invitrogen=2750000
                     vntifkey=4"
     primer_bind     739..761
                     /label=SpnR-F1
     primer_bind     complement(783..804)
                     /label=SpnR-R2
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=pUC origin
                     invitrogen=2530000
                     vntifkey=33"
     terminator      2319..2362
                     /label=rrnB T1 terminatior terminator
                     /label=rrnB T1 terminatior
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=rrnB T1 terminatior
                     vntifkey=43"
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2560
                     /label=attL1
                     /note="ApEinfo_fwdcolor=#00ff40
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=attL1
                     invitrogen=2670000
                     vntifkey=86"
     gene            2579..2629
                     /label=N-terminal NLS
                     /note="N-terminal NLS
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2585..2605
                     /label=SV40 NLS
                     /note="SV40 NLS
                     ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0000"
     misc_feature    2630..6526
                     /label=FnCpf1
                     /note="ApEinfo_fwdcolor=#00ff46
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     primer_bind     complement(4419..4477)
                     /label=239-RVR-R1
     primer_bind     4445..4503
                     /label=239-RVR-F2
     misc_feature    4445..4447
                     /label=N623R
     misc_feature    4463..4465
                     /label=K629V
     misc_feature    4475..4477
                     /label=N633R
     misc_feature    6527..6574
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS
                     ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0000"
     misc_feature    6575..6577
                     /label=stop
                     /note="stop
                     ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0000"
     misc_feature    6603..6880
                     /label=NOS term
                     /note="ApEinfo_fwdcolor=#f5010a
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=NOS term
                     vntifkey=21"
     misc_feature    complement(6647..6670)
                     /label=Nos-term-R2
                     /label=PCR_primer
                     /note="Nos-term-R2
                     ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_recomb     6902..7025
                     /label=attR5
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     dnas_title=attR5
                     invitrogen=2703000
                     vntifkey=86"
     primer_bind     complement(7066..7082)
                     /label=M13 reverse primer
     promoter        7154..7155
                     /label=Spn promoter(1) promoter
                     /label=Spn promoter(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=Spn promoter
                     ApEinfo_revcolor=pink
                     dnas_title=Spn promoter
                     vntifkey=30"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggctcctaag aagaagcgga aggttggtat tcacggggtg cctgcggctt cgatctatca
     2641 agaattcgtt aataagtatt cgctttctaa aacactgaga ttcgaactca tacctcaagg
     2701 aaagacactc gagaacataa aggctagggg cctcatattg gatgatgaaa agagagccaa
     2761 ggactataaa aaagccaaac agataatcga caagtaccac caattcttca tagaagaaat
     2821 cctttccagc gtctgcatta gcgaggattt gttgcaaaat tacagcgatg tctacttcaa
     2881 gcttaagaag agcgatgacg acaatctcca gaaagacttc aaatcagcga aggacacaat
     2941 taagaagcag atcagcgagt atatcaaaga tagtgaaaaa ttcaagaacc ttttcaatca
     3001 gaacctgata gacgcaaaaa aaggacaaga aagcgacctc attctttggt tgaagcagtc
     3061 taaggacaac gggattgagc tttttaaggc gaatagcgat ataaccgaca tcgacgaggc
     3121 gcttgagatc ataaagtcgt ttaagggatg gacaacatac ttcaaaggct tccatgagaa
     3181 tcgcaaaaac gtctactcca gcaacgacat tccaacgtcg attatataca gaattgttga
     3241 tgataacctc cctaaattcc tcgaaaataa ggcaaaatat gaaagcctta aagataaagc
     3301 gcctgaagca atcaattatg aacaaatcaa aaaggatctt gctgaagaat tgacgtttga
     3361 tatagactac aagacgtcag aagttaacca gagggtgttt tcactcgacg aggtgtttga
     3421 gattgctaat ttcaacaact acctcaatca gagtgggatc acgaagttca acactattat
     3481 aggtggtaag ttcgtgaatg gtgagaacac taaaagaaaa gggattaacg aatatataaa
     3541 cctttatagt caacagatca acgacaagac tttgaagaaa tataagatga gcgtcctctt
     3601 caagcagata ctcagtgaca cggaatccaa gagctttgtg atcgacaagc tcgaagatga
     3661 ctcggatgtg gtcactacca tgcaatcctt ctacgagcaa attgccgctt tcaaaactgt
     3721 ggaggagaag agtataaagg agacactgtc tctgcttttt gacgatctta aggcccagaa
     3781 attggatctt tccaaaatat atttcaagaa tgataagtca cttacggacc tttcccaaca
     3841 agtttttgac gattattcag ttattggtac ggcggttctt gagtacatta cgcagcagat
     3901 agcccccaag aatctggaca acccctctaa gaaagaacag gagttgatag cgaagaagac
     3961 agagaaggcg aaatacctct cgctggagac cataaaattg gcactggaag aatttaacaa
     4021 gcatcgcgac atagataaac agtgccgctt cgaggaaatt ttggcaaatt ttgcagccat
     4081 tccaatgatt ttcgacgaga tagcgcaaaa caaggataat ttggcacaaa tttcaataaa
     4141 atatcaaaac cagggcaaga aggacctctt gcaggcttcg gcagaggatg atgttaaggc
     4201 tattaaagac ttgttggacc aaacgaacaa tctgttgcac aagttgaaaa ttttccacat
     4261 tagtcaaagc gaggataaag caaacatatt ggacaaagac gagcactttt atctggtgtt
     4321 tgaggagtgc tacttcgagc ttgctaatat tgtcccactt tataataaaa taagaaacta
     4381 cattacgcaa aagccatatt cagatgaaaa gtttaagctc aatttcgaaa atagtactct
     4441 tgcccgcggc tgggacaaga atgtggagcc agataggacc gccatacttt ttatcaaaga
     4501 tgataaatat tatcttgggg tgatgaataa gaagaataat aagatcttcg atgataaagc
     4561 gataaaggaa aataagggtg aaggctataa aaaaattgtt tacaaactgt tgccgggagc
     4621 aaataaaatg ctccccaagg tttttttttc ggcaaagagc attaaatttt acaatccttc
     4681 agaagacatt ctgcgcataa gaaatcattc gacacacact aaaaatggtt cgccacaaaa
     4741 gggctacgag aaatttgaat tcaacattga ggactgtcgg aagttcattg atttctacaa
     4801 gcagtccatc tccaagcacc cggagtggaa agattttggg tttcggtttt ccgacacgca
     4861 gagatacaac agcattgatg aattttatag agaggtcgag aatcaaggtt ataagcttac
     4921 ctttgaaaac atttctgaat catacattga ttcagtggtc aatcagggca aactctatct
     4981 ttttcaaata tacaacaagg actttagtgc ttatagtaaa gggcggccca atttgcatac
     5041 tctctattgg aaagcgctgt ttgatgagcg gaaccttcaa gacgtcgtgt ataagctcaa
     5101 cggggaagcc gagctctttt accgcaagca gtccataccg aaaaaaataa cacaccctgc
     5161 caaagaagcc atcgccaaca agaataaaga caatcctaaa aaagagtccg tcttcgaata
     5221 tgatcttatt aaggacaaga ggtttacaga agataaattt ttcttccatt gtcccataac
     5281 tatcaatttc aaaagctctg gcgcgaacaa atttaacgac gaaatcaatc tcttgttgaa
     5341 agaaaaagcc aacgatgtgc acattctgtc gatcgacagg ggagagcgcc acttggcata
     5401 ctacaccctt gttgatggga aaggaaatat tattaaacag gacacattta atatcatcgg
     5461 caacgatcgc atgaagacca actatcatga caaactggca gcaattgaaa aggaccgcga
     5521 ctcagcgaga aaagactgga agaagatcaa taatatcaaa gaaatgaaag agggttattt
     5581 gtctcaagtg gtccatgaga tcgcgaagtt ggtcattgaa tataatgcca tagtggtctt
     5641 cgaagatctg aattttggat ttaagcgcgg caggttcaaa gtcgaaaaac aggtctacca
     5701 aaagttggaa aagatgctca tcgaaaagct gaattacctt gtcttcaaag ataacgaatt
     5761 cgataaaacc gggggggtct tgagggccta ccaactgact gcaccctttg agacttttaa
     5821 aaagatgggt aaacagacag gaataattta ctatgttcct gccggtttca ctagcaagat
     5881 ttgccccgtt accggattcg tgaatcaact ctatcccaaa tacgaatccg tgagcaagag
     5941 tcaggaattc ttctccaaat ttgataaaat atgctataat ctcgacaaag gttatttcga
     6001 gttctcgttc gactataaga acttcgggga taaggctgcc aagggaaagt ggactatagc
     6061 aagctttggt agtcgcctta taaattttag gaacagcgac aagaatcaca actgggacac
     6121 tcgggaagtc tacccaacaa aagaactgga gaaactcttg aaggattata gtatcgagta
     6181 tgggcatggg gagtgtatca aggcagcgat ttgtggagag tccgacaaaa agttttttgc
     6241 taaactcacc tcggtgctca acactatcct ccagatgaga aattcaaaaa cagggacaga
     6301 gctcgattac ctcattagcc ccgttgccga cgtcaatgga aactttttcg actcaagaca
     6361 ggctccaaaa aacatgccgc aagatgcgga cgcgaatggg gcctatcaca taggcctgaa
     6421 agggcttatg ctccttggga gaattaaaaa taaccaagaa ggcaaaaaac tcaacctcgt
     6481 cattaagaac gaagaatact tcgaatttgt tcagaacagg aataacaagc gtcctgctgc
     6541 caccaaaaag gccggacagg ctaagaaaaa gaagtgagac gactagtggc ggccgccgac
     6601 gtccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     6661 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     6721 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     6781 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6841 atctatgtta ctagatcggg aattgatccc ccctcgacag cttccggaaa gggcgaattc
     6901 gcaactttgt atacaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
     6961 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     7021 ctatgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     7081 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     7141 tcatcatgcc tcctc
//
