LOCUS 19_p5xU6_5sgRNA_ 13822 bp ds-DNA circular 07-MAR-2018 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 125..982 /SerialCloner_Color=&h8080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=ampR /ApEinfo_fwdcolor="#ffff66" /ApEinfo_revcolor="#ffff66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1130..1803 /SerialCloner_Color=&h80FF80 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=pUC ori /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2272..2290 /SerialCloner_Color=&h0080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=Tol2 5' TIR /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2847..3044 /SerialCloner_Color=&hFF80C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=SV40 late PolyA /ApEinfo_fwdcolor="#008080" /ApEinfo_revcolor="#008080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5209..5212 /label=5'overhang /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3596..3601 /label=U6 end signal /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3048..3053 /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=ClaI site /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5094..5112 /label=site8 /ApEinfo_fwdcolor="#ffcc66" /ApEinfo_revcolor="#ffcc66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter complement(11187..12703) /label=hsp70l promoter (AF158020) /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#ff8000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5610..5628 /label=site9 /ApEinfo_fwdcolor="#ffcc66" /ApEinfo_revcolor="#ffcc66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal complement(5799..5994) /label=SV40 late polyA (from pCS2+) /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#ff8000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5747..5752 /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=KpnI site /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 12704..12726 /label=LR recombined arm /ApEinfo_fwdcolor="#b3b3b3" /ApEinfo_revcolor="#b3b3b3" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5118..5198 /label=sgRNA scaffold /ApEinfo_fwdcolor="#6666ff" /ApEinfo_revcolor="#6666ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6920..6925) /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=BamHI site /ApEinfo_fwdcolor="#cc66ff" /ApEinfo_revcolor="#cc66ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(3089..3335,3337..3485) /label=zf_U6a promoter /ApEinfo_fwdcolor="#66ffff" /ApEinfo_revcolor="#66ffff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6830..6883) /SerialCloner_Color=&h0000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=T2A /ApEinfo_fwdcolor="#ffcc66" /ApEinfo_revcolor="#ffcc66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4599..4679 /label=gRNA scaffold /ApEinfo_fwdcolor="#6666ff" /ApEinfo_revcolor="#6666ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5715..5720 /label=U6 end signal(1) /ApEinfo_label="U6 end signal" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6926..11074) /SerialCloner_Color=&hFF00FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=Cas9 /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4575..4593 /label=site7 /ApEinfo_fwdcolor="#ffcc66" /ApEinfo_revcolor="#ffcc66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6824..6829) /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=XbaI site /ApEinfo_fwdcolor="#cc66ff" /ApEinfo_revcolor="#cc66ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6899..6919) /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=nls2 /ApEinfo_fwdcolor="#66ffff" /ApEinfo_revcolor="#66ffff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4690..4693 /label=5'overhang(1) /ApEinfo_label="5'overhang" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6104..6823) /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=GFP /ApEinfo_fwdcolor="#80ff00" /ApEinfo_revcolor="#80ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5213..5604 /label=zf_U6d promoter /ApEinfo_fwdcolor="#66ffff" /ApEinfo_revcolor="#66ffff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(11042..11062) /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=nls1 /ApEinfo_fwdcolor="#66ffff" /ApEinfo_revcolor="#66ffff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(3619..3866,3868..4016) /label=zf_U6a promoter(1) /ApEinfo_label="zf_U6a promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(6096..6103) /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=NotI site /ApEinfo_fwdcolor="#ff66ff" /ApEinfo_revcolor="#ff66ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3602..3605 /label=5'overhang(2) /ApEinfo_label="5'overhang" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5199..5204 /label=U6 end signal(2) /ApEinfo_label="U6 end signal" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5777..5798 /label=LR recombined arm(1) /ApEinfo_label="LR recombined arm" /ApEinfo_fwdcolor="#b3b3b3" /ApEinfo_revcolor="#b3b3b3" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3072..3075 /label=BsaI 5'overhang /ApEinfo_fwdcolor="#ff6fcf" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4137..4140 /label=5'overhang(3) /ApEinfo_label="5'overhang" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5725..5728 /label=BsaI 3'overhang /ApEinfo_fwdcolor="#ff6fcf" /ApEinfo_revcolor="#ff6fcf" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4694..5088 /label=zf_U6c promoter /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4680..4685 /label=U6 end signal(3) /ApEinfo_label="U6 end signal" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5634..5714 /label=sgRNA scaffold(1) /ApEinfo_label="sgRNA scaffold" /ApEinfo_fwdcolor="#6666ff" /ApEinfo_revcolor="#6666ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4022..4040 /label=site6 /ApEinfo_fwdcolor="#ffcc66" /ApEinfo_revcolor="#ffcc66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3515..3595 /label=sgRNA scaffold(2) /ApEinfo_label="sgRNA scaffold" /ApEinfo_fwdcolor="#6666ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4046..4126 /label=gRNA scaffold(1) /ApEinfo_label="gRNA scaffold" /ApEinfo_fwdcolor="#6666ff" /ApEinfo_revcolor="#6666ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3054..3071 /label=Tol2 exon4 frag from previous cloning step /ApEinfo_fwdcolor="#b3b3b3" /ApEinfo_revcolor="#b3b3b3" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4127..4132 /label=U6 end signal(4) /ApEinfo_label="U6 end signal" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3491..3509 /label=site5 /ApEinfo_fwdcolor="#ffcc66" /ApEinfo_revcolor="#ffcc66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4141..4569 /label=zf_U6b promoter /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 12769..13357 /label=Tol2 /ApEinfo_fwdcolor="#e6e6e6" /ApEinfo_revcolor="#e6e6e6" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 13293..13308 /label=Tol2 3'TIR /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4360..4382 /label=U6bseqF /ApEinfo_fwdcolor="#e6e6e6" /ApEinfo_revcolor="#e6e6e6" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 61 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 121 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 181 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 241 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 301 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 361 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 421 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 481 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 541 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 601 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 661 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 721 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 781 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 841 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 901 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 961 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 1021 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 1081 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 1141 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 1201 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 1261 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 1321 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 1381 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 1441 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 1501 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 1561 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 1621 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 1681 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 1741 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 1801 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 1861 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 1921 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 1981 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 2041 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 2101 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 2161 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 2221 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT 2281 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA 2341 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG 2401 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT 2461 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC 2521 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA 2581 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA 2641 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT 2701 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA 2761 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTGATCTA GAGGATCATA ATCAGCCATA 2821 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA 2881 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 2941 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 3001 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCATCATC GATACATTAA 3061 ATGAAATGCA Tcagtaagtc tctcagcgTT TCTCCAGCCT CGGTCATTCA GTAAGACGAG 3121 TTTCCTGCTC TCGGCGTCTT TTGTTCTGGT CTTCAAGGAG GGGGGAAATG TTCTGCGCAT 3181 GCCTGTGGGG GAGGGAGAAG GACACGTCAC TGAAAACGTC CCTGCATCAC ACCGAGACAC 3241 ccaatCACTC AAGCCaAGAC CAGATAATTT TGCATATGCT TTACAGTTTG AAAATTACCA 3301 CGGTAATCCC TCACACAAAC TCTGGATTTG AGATCcTTCA GGTTTTATCA GTTTGCAGGT 3361 TTATGTCACC ATGATATAGG GTCAGACTTG ATCTAAGGGA GCTGAATAAG TGGTTTAGTC 3421 ACTCACCACC TCCCAAAAAC ATACccagaa gtccctggta tatatagctC TCCCTCCAGC 3481 TCTTGgTTCG AGAGCATAGA CATCGAGTAG TTTaAGAGCT ATGCTGGAAA CAGCATAGCA 3541 AGTTTAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT 3601 Tagtcaagtc tctcagcgTT TCTCCAGCCT CGGTCATTCA GTAAGACGAG TTTCCTGCTC 3661 TCGGCGTCTT TTGTTCTGGT CTTCAAGGAG GGGGGAAATG TTCTGCGCAT GCCTGTGGGG 3721 GAGGGAGAAG GACACGTCAC TGAAAACGTC CCTGCATCAC ACCGAGACAC ccaatCACTC 3781 AAGCCaAGAC CAGATAATTT TGCATATGCT TTACAGTTTG AAAATTACCA CGGTAATCCC 3841 TCACACAAAC TCTGGATTTG AGATCTcTTC AGGTTTTATC AGTTTGCAGG TTTATGTCAC 3901 CATGATATAG GGTCAGACTT GATCTAAGGG AGCTGAATAA GTGGTTTAGT CACTCACCAC 3961 CTCCCAAAAA CATACccaga agtccctggt atatatagct CTCCCTCCAG CTCTTGgTTC 4021 GACTACAGTC GCTACGACTA GTTTaAGAGC TATGCTGGAA ACAGCATAGC AAGTTTAAAT 4081 AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTAAActca 4141 TTACCCTCCA CGTGTCTGTC TGGGTTTTCA TGGGCTCTGC TCTAGTGAGA GCAGCTTCCT 4201 TTTGTCGGAG TGTTTTGTGC TCTTATCCAA GGCAGGGGGG ATCTGCGCAT GCCTGTGGGG 4261 GGAGGAGAAG GACACGTGAA CAAAAGCTCC TCGATGTCAC ACAGGAAGTT CAGGAACTCA 4321 TCCAATCACT CTAAAGAAAC GGCCTGTTTC CTTCGCATAC GCTTACAGCT CCAAAACTCT 4381 ACGGTAAACC TACATAAACT GCTGGTTTTC AAATTTTAAA GAATTTAAGG GTTTACAGGT 4441 TTACTACTAC ACAGTGATTT ACTGACACAT GTAAGTGTAA ATGAGTTGAA TAAGTAAGTA 4501 AGCCATATAC CACACATGAA ACACATACCC AGAAGTCACT GGTATATATA GCCGTCCTCC 4561 AGACTCCCAg TTCGACAGAG ATATCATGCA GTAGTTTaAG AGCTATGCTG GAAACAGCAT 4621 AGCAAGTTTA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCT 4681 TTTTTTAAAc caaTCCGAGA GTCTGTGAAT GTTTCGATGG GCCGTTTGCT GGCGTCTGAG 4741 CATGTTGTCC TCTGGTCTGC GGCGGGGGAA GGTCCGCGCA TGCCTGTGGG GGAAGGCGAA 4801 GGACACGAGG ACAAAAGATC AGTTACGTCA CAGAAGAACT CATCCAATCA CACAAGCCTA 4861 AAACCAGCTT CCTCTGCATA TGCTTTACAG TTCAAAAATA CTACGGTAAA CCTCTACAAA 4921 ACTGCTGGTT TCAAACTTTG AAGGTTTTAA GCGTTTGCAG GTTTGCCCCG AAGAGGTTTA 4981 CTGTCATGTT TGAGGTAAAT GAGTTGAATA AGTAGGTTTA TCCACTTACC ACATGGCAGA 5041 AACATACCCA GAAGTCCCGG GTTTATATAG CAGTTCTCCA GGCTCCTAgT TCGACAGCAG 5101 TATCTGCTGT CAGTTTaAGA GCTATGCTGG AAACAGCATA GCAAGTTTAA ATAAGGCTAG 5161 TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTTAAAcc tgTGATTTGG 5221 TGGTTGTGAA AGGAGCATTT GCTGGTCTTT CAACCTTTTG TTCTCGTCAT CGTTGAGGGG 5281 GAAACATTCT ACGCATGTCT GTGGGGGAGG GTGTGGAACA TTGTGGCCAC GGGTTCTTCA 5341 CTGTCACACC GAAACATCCA ATCACAGCTA GCAGAGAGGA ACCTTTTTTA CATGTGGTCT 5401 ACAGCTGAGG AAATACTCAA AATAAACCTT CCACAAAAAC ATTGGCTTGC AATTTTGCAG 5461 ATCATAAGAG TTTACAGGTC TATCTCACCA GTGTATACAT TCAAATTTGT TGGAAGCTAA 5521 TTAAATAAGG GGTGTATCTA CTTACCACCA GTAAGAAACA TACCCAGAAT TCCCTGGTAT 5581 ATATAGCTCT TCTTCCGGCT CTCAgTTCGA CTGCACGACA GTCGACTAGT TTaAGAGCTA 5641 TGCTGGAAAC AGCATAGCAA GTTTAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG 5701 CACCGAGTCG GTGCTTTTTT TAAAgttcgt acCattaata gtgtgcGGTA CCGTAAAACG 5761 ACGGCCAGTG AATTATCAAC TATGTATAAT AAAGTTGGAA AAAACCTCCC ACACCTCCCC 5821 CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT 5881 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 5941 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCCCCAC 6001 TTTGTACAAG AAAGCTGGGT GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA 6061 CTAAAGGGAA CAAAAGCTGG AGCTCCACCG CGGTGGCGGC CGCTTACTTG TACAGCTCGT 6121 CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT 6181 TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA 6241 GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC 6301 CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT GCCGTTCTTC TGCTTGTCGG 6361 CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC 6421 CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA 6481 ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT 6541 GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT 6601 AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG 6661 GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT 6721 CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA 6781 TGGGCACCAC CCCGGTGAAC AGCTCTTCGC CCTTGCTCAC CATTCTAGAA GGGCCGGGAT 6841 TCTCTTCCAC GTCACCGCAT GTCAGCAGAC TGCCTCTGCC CTCTCCGCCA CCAGACCTCA 6901 CCTTTCTCTT CTTCTTAGGG GATCCCACCG GTGAGTCGCC ACCCAGCTGA GACAGGTCAA 6961 TCCTTGTTTC GTACAGGCCG GTAATACTCT GATGGATCAG TGTAGCGTCC AGCACCTCCT 7021 TAGTGGAGGT GTACCTCTTT CTATCGATTG TAGTGTCGAA GTACTTGAAA GCTGCAGGTG 7081 CGCCCAGGTT TGTCAGAGTG AACAGATGAA TGATATTTTC GGCCTGCTCC CTGATGGGCT 7141 TATCTCTGTG TTTGTTGTAA GCAGACAGGA CCTTATCCAG ATTTGCGTCG GCCAGGATCA 7201 CTCTTTTAGA GAACTCGCTA ATCTGTTCAA TGATCTCGTC CAGATAATGC TTGTGCTGCT 7261 CCACAAACAG CTGTTTCTGT TCGTTATCCT CTGGAGATCC TTTCAGCTTC TCGTAGTGGC 7321 TTGCCAGATA CAGGAAGTTC ACGTACTTAG AAGGCAGGGC CAGTTCATTG CCTTTCTGCA 7381 GCTCTCCAGC GCTTGCCAGC ATGCGTTTCC TGCCGTTTTC CAGCTCAAAC AGACTGTACT 7441 TGGGCAGTTT AATGATCAGG TCTTTCTTCA CTTCCTTATA ACCTTTGGCC TCCAGAAAAT 7501 CGATTGGGTT CTTCTCGAAA GAGCTTCTCT CCATAATTGT GATACCCAGC AGCTCCTTGA 7561 CAGATTTCAG TTTCTTGCTT TTGCCCTTCT CCACCTTAGC GACCACCAGG ACGGAGTATG 7621 CCACAGTGGG AGAGTCAAAG CCTCCATACT TCTTAGGGTC CCAGTCTTTC TTTCTAGCGA 7681 TCAGCTTATC AGAGTTGCGT TTTGGCAGAA TACTTTCTTT TGAGAAACCG CCTGTCTGCA 7741 CCTCAGTTTT CTTGACGATA TTCACTTGAG GCATGCTCAG GACCTTCCTC ACGGTTGCAA 7801 AATCTCTGCC TTTGTCCCAC ACGATTTCGC CTGTCTCTCC GTTTGTCTCA ATCAGGGGCC 7861 TTTTGCGGAT TTCTCCATTG GCCAGGGTGA TCTCTGTCTT AAAGAAATTC ATGATGTTTG 7921 AGTAAAAGAA ATACTTGGCA GTAGCTTTTC CAATTTCCTG CTCGGACTTG GCGATCATTT 7981 TTCTGACATC GTACACCTTA TAGTCTCCGT ACACGAATTC GCTCTCCAGT TTTGGGTATT 8041 TCTTGATCAG TGCGGTACCG ACCACGGCGT TCAGGTATGC GTCATGGGCA TGGTGATAAT 8101 TGTTAATCTC GCGGACCTTA TAAAACTGGA AATCTTTCCT AAAGTCGCTG ACCAGTTTAC 8161 TCTTCAGGGT AATGACCTTC ACTTCTCTGA TCAGTTTGTC ATTCTCATCG TACTTTGTGT 8221 TCATGCGACT ATCCAGAATC TGTGCGACGT GTTTAGTGAT CTGGCGTGTT TCCACCAGCT 8281 GCCTCTTGAT GAATCCAGCT TTGTCCAGTT CTGACAGTCC ACCGCGCTCT GCTTTTGTCA 8341 GGTTATCAAA CTTCCTCTGA GTGATCAGTT TGGCATTCAG CAGCTGTCTC CAGTAGTTCT 8401 TCATTTTCTT GACCACTTCC TCACTAGGGA CATTATCTGA TTTTCCTCTG TTTTTGTCGG 8461 ACCTGGTCAG CACCTTGTTG TCAATGCTGT CATCTTTGAT GAAACTCTGG GGGACGATAT 8521 GATCCACGTC GTAATCAGAC AGCCTGTTAA TATCCAGCTC CTGGTCCACG TACATATCTC 8581 TTCCATTCTG CAGATAGTAC AGATACAGCT TCTCATTCTG CAGCTGTGTG TTTTCCACTG 8641 GGTGCTCTTT CAGGATCTGG CTACCCAGCT CCTTAATGCC TTCCTCGATT CTTTTCATCC 8701 TCTCTCTACT GTTCTTCTGA CCCTTCTGGG TTGTCTGATT TTCGCGAGCC ATCTCAATCA 8761 CGATGTTTTC GGGCTTATGG CCCATGACTT TCACCAGCTC ATCGACGATT TTCACTGTCT 8821 GCAGAATGCC TTTCTTGATG GCTGGGCTTC CAGCCAGGTT TGCAATCTGC TCATGCAGGG 8881 AGTGGCCCTG TCCAGACACC TGAGCCTTCT GGATGTCCTC TTTGAAGGTC AGACTGTCAT 8941 CGTGAATCAG CTGCATAAAA TTGCGGTTGG CGAAGCCATC ACTTTTCAGA AAGTCCAGGA 9001 TTGTCTTTCC TGACTGTTTA TCCCTAATGC CGTTGATCAG CTTCCTGCTC AGTCTACCCC 9061 AGCCAGTGTA GCGCCTTCTC TTCAGCTGTT TCATCACCTT GTCATCGAAC AGATGAGCAT 9121 AGGTTTTCAG TCTTTCCTCG ATCATTCCTC TGTCCTCAAA CAGGGTCAGT GTCAGCACAA 9181 TGTCTTCCAG GATATCCTCA TTTTCCTCGT TGTCCAGGAA GTCTTTATCC TTAATGATCT 9241 TCAGCAGATC GTGGTAAGCG CCCAGGCTTG CGTTAAATCT GTCTTCGACT CCGCTGATCT 9301 CCACGGAATC GAAGCATTCA ATTTTCTTGA AGTAGTCCTC CTTCAGCTGC TTGACAGTCA 9361 CTTTCCTGTT GGTTTTAAAC AGCAGATCCA CGATTGCTTT CTTCTGCTCT CCTGACAGGA 9421 AGGCTGGCTT TCTCATGCCC TCGGTGACGT ATTTCACCTT TGTCAGTTCG TTATAGACAG 9481 TGAAATACTC GTACAGCAGG GAGTGCTTAG GCAGCACTTT CTCATTGGGC AGGTTTTTGT 9541 CAAAATTAGT CATTCTCTCG ATGAAGGACT GTGCAGAGGC GCCCTTATCG ACCACTTCCT 9601 CGAAGTTCCA AGGAGTAATG GTTTCCTCGG ATTTGCGAGT CATCCAGGCA AATCTGCTGT 9661 TTCCGCGAGC CAGTGGACCC ACATAGTAAG GGATTCTGAA GGTCAGAATT TTTTCGATCT 9721 TCTCGCGGTT ATCTTTCAGA AAGGGGTAGA AGTCTTCCTG GCGCCTCAGG ATAGCGTGCA 9781 GCTCTCCCAG ATGAATCTGG TGTGGGATGG AACCGTTATC AAATGTGCGC TGCTTCCTCA 9841 GCAGGTCCTC TCTATTCAGT TTCACCAGCA GTTCCTCAGT TCCGTCCATC TTCTCCAGAA 9901 TAGGTTTGAT AAACTTGTAG AATTCCTCCT GTGATGCGCC TCCGTCGATA TAACCGGCGT 9961 AGCCGTTCTT GGACTGATCA AAGAAAATTT CCTTGTACTT CTCTGGCAGC TGCTGCCTGA 10021 CCAGAGCCTT CAGCAGGGTC AGATCCTGAT GGTGCTCGTC ATATCTCTTG ATCATGCTGG 10081 CACTCAGAGG TGCTTTTGTA ATCTCGCTGT TCACTCTCAG AATATCTGAC AGCAGGATTG 10141 CGTCGGACAG ATTTTTGGCA GCCAGGAACA GGTCAGCGTA CTGATCTCCG ATCTGTGCCA 10201 GCAGGTTATC CAGGTCATCG TCATATGTGT CCTTTGACAG CTGCAGTTTG GCGTCCTCAG 10261 CCAGATCAAA ATTGCTTTTG AAGTTAGGAG TCAGGCCCAG ACTCAGAGCG ATCAGATTTC 10321 CAAACAGACC GTTCCTCTTT TCTCCGGGCA GCTGTGCAAT CAGGTTCTCC AGTCTGCGTG 10381 ACTTGGACAG CCTGGCTGAC AGGATAGCTT TTGCGTCCAC TCTGGAAGCA TTAATAGGGT 10441 TTTCCTCAAA CAGCTGATTG TAAATCTGGA CCAGCTGGAT GAACAGCTTG TCCACATCGG 10501 AATTGTCGGG GTTCAGATCG CCCTCGATCA GAAAATGACC CCTGAACTTA ATCATGTGGG 10561 CCAGAGCCAG ATAGATCAGT CTCAGGTCGG CTTTATCAGT AGAGTCAGCC AGTTTCTTGC 10621 GCAGATGATA GATGGTTGGG TATTTTTCGT GATAGGCGAC CTCGTCCACA ATGTTTCCAA 10681 AGATAGGATG GCGCTCGTGT TTCTTATCTT CCTCGACCAG GAAACTTTCC TCCAGCCTAT 10741 GAAAGAATGA ATCGTCCACC TTAGCCATTT CGTTAGAGAA AATCTCCTGC AGGTAACAGA 10801 TTCTATTCTT CCTTCTGGTA TAGCGCCTTC TTGCTGTTCT TTTCAGGCGT GTTGCTTCGG 10861 CAGTCTCTCC AGAACCGAAC AGCAGGGCTC CAATCAGATT TTTCTTGATG CTGTGCCTGT 10921 CGGTGTTACC CAGCACCTTA AATTTCTTGC TAGGGACCTT GTAGTCGTCT GTAATCACTG 10981 CCCAACCCAC GGAGTTAGTT CCAATATCCA GGCCGATGCT ATACTTCTTA TCCATGCTTC 11041 CCACCTTTCT CTTCTTCTTA GGTGGAGAAG CCATGGTGGC GTCGACCTCG AGGGGGGGCC 11101 CGGTACCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC GTCGTTTTAC 11161 AAGCCTGCTT TTTTGTACAA ACTTGGCAGG AAAAAAAAAC AATTAGAATT AATTTTATAT 11221 TTATACAATT TATGGTGCAA TTGTTTCATT ATGAAAGGAT TTTTCTAGTC AACAAGTGAA 11281 ATTCTCTTAA TAACCAAGTT ATTATTAGTT GTACAAGTAA AACGCTAATT TTCTTTTGTA 11341 GACAACATTA ACACAAATTC ATTTAGGACA TTTGCTAGTA TTTATGGTTA TTATGCTCAT 11401 ATAAAATAAT GTCAGCAACA TAAAGTAAAT GCCCGTCTGT TCATTGCTTT TTTTAAAAAT 11461 CCTTATTGTG AAAACGGGAA ATATAATTTT CTAGTAGACC TAAAATAAGA TGAACATAGG 11521 CAGTCCACAT GTAAAAAGAT TACATATACA AAGTTGATTT TTTGACATTA AGTACATATA 11581 ATCTCTATAT AATTCTGATT AGCCAATAGA GTACAATATA TTGAGACTTA CCTTACAGTA 11641 GATTCTTAAA TAGAGCGAAT AAACACCTCG TCGGGGAAAA AGTCCTGTCA GGCTGCTCTT 11701 TTCTTCTTTG TCAAGTCGCT TCTCTTCGGT TGAGTTTCTC TGCGGTCTCT CTTTCGCCCT 11761 TTAAATACAG CAGAGCGCGC ACCCGTTCAG ACTCTACACT GTTCTGGAAG CCTCCAGTGA 11821 ATTCGACCAA TCAAAACACA GCAGGCAGCC GCGTCACTTC TTCAGCTGTC ATTGAGAGGA 11881 GCCGCTGAGA GGAATGTAGT GGAGATTTCC AGAGTTTTAC CGTGAAATTA CGCGAAACAG 11941 CGGTCAAAAA TACAAAGAGA TTTAAAATGT TTTTTTTATT TGAGTGTTTT ATTGTACTTA 12001 TTGCTGAAAT ATGTGCGGTT GTGTGATGTG TTGTTTTTCC AGTTGCAATG AATGAACAAA 12061 CTAAAATGAT AAAATGTTTG AACGATTCAG TAAAAAAAAA TGCAATAAAA AAATCTTGCT 12121 GAAATGTTTT ACACAGTAGC AAAATCGGGC CTGTGGTGTT TTCAGCTACA TAGAAGGCAA 12181 ACCTGTTTTA TATAAGCTAA TAGCTTTGGT CAGATTTGTT TAACGTTTCA ACAAAACAAA 12241 TGGATGTTTA CTGTACTATA TTGATTGGTT AAAACCAATA CTTTATTCTG GGATTGCAGT 12301 CTTAACTATT TTTGTAGTGT TAAAACCAAC TTGCTAACTT GAGAGGTCAT GTTTACCCTC 12361 AGAGAAATAG GCACAAGTCT CTTTGTTTAA TATTGGAGAT CATCCTCCTT GTTCAGTCGT 12421 GGTCTGTATT TAATTTTAAT GTATATGATT GCCGTAAAAG TGTTAGAAAG TTTACCCTGG 12481 TGCTATGAAA TCAATCTGCT GCTTTTGACC CAGTTAGTGT GTCTTTAATC TAACTTAATG 12541 AGACCCCAGT GTGGTCAACA ATCAATAGAA ACAAAGTGTG TTGTTTTATA ACTGTTAACT 12601 CCTATTATTT TTTATCTTCT GTAGCATATA GACAAAACAT GCTCGTTCTA ATAGTTTAAT 12661 AAAATTAATT TGATTTTTGG AAATAACCAA GCGACACCCC TGAAGCAACT TTTCTATACA 12721 AAGTTGATAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGCTCGAGGG CCCATCTGGC 12781 CTGTGTTTCA GACACCAGGG AGTCTCTGCT CACGTTTCCT GCTATTTGCA GCCTCTCTAT 12841 CAAGACTAAT ACACCTCTTC CCGCATCGGC TGCCTGTGAG AGGCTTTTCA GCACTGCAGG 12901 ATTGCTTTTC AGCCCCAAAA GAGCTAGGCT TGACACTAAC AATTTTGAGA ATCAGCTTCT 12961 ACTGAAGTTA AATCTGAGGT TTTACAACTT TGAGTAGCGT GTACTGGCAT TAGATTGTCT 13021 GTCTTATAGT TTGATAATTA AATACAAACA GTTCTAAAGC AGGATAAAAC CTTGTATGCA 13081 TTTCATTTAA TGTTTTTTGA GATTAAAAGC TTAAACAAGA ATCTCTAGTT TTCTTTCTTG 13141 CTTTTACTTT TACTTCCTTA ATACTCAAGT ACAATTTTAA TGGAGTACTT TTTTACTTTT 13201 ACTCAAGTAA GATTCTAGCC AGATACTTTT ACTTTTAATT GAGTAAAATT TTCCCTAAGT 13261 ACTTGTACTT TCACTTGAGT AAAATTTTTG AGTACTTTTT ACACCTCTGT CAAGAACTCC 13321 TGGACAAACC TCTGACCTGT GTGGAACAGA GTGGATATGG GTGTCTGAAC AGATATTCAC 13381 GTCTTTTGCA GATCAGAGGG CATTTCTGGT GTTGTGCAAT GACCTGGGTC CAACAGGTAG 13441 GCGTACTCGT ATCCAAGAGC CTTGTTGGAC AGGAGGTAGT CTCCATTCCT ATAAAGAGGA 13501 AGGAATGGCA CCATGTAGTA GCCGTCATTG TGGCCAATTG GTGCATTGGT ACGTGGGTAG 13561 ATGGACCGGG GAGGCTGATG AGTTCTTAGC CAGCGTGGGC CATCGCCCTG ATAGACGGTT 13621 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 13681 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 13741 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA 13801 TTAACGCTTA CAATTTAGGT GG //