LOCUS       MPRA:minP:GFP           3356 bp ds-DNA     circular     16-JUN-2021
DEFINITION  .
ACCESSION   urn.local...33d4wqf-c8                                             
VERSION     urn.local...33d4wqf-c8                                             
SOURCE      other sequences; artificial sequences; vectors                     
  ORGANISM  other sequences; artificial sequences; vectors. .                  
FEATURES             Location/Qualifiers
     rep_origin      complement(1..620)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          54..73
                     /label="pGL4.23_backbone_2"
                     /note="sequence: ATCGACGCTCAAGTCAGAGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(77..97)
                     /label="pGL4.23_backbone_1"
                     /note="sequence: TTATAGTCCTGTCGGGTTTCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(619..660)
                     /label="pGL4.23:SV40E_kpnI_removal_F"
                     /note="sequence: CGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(641..660)
                     /label="pGL4.23_lin-ori/ampR_R"
                     /note="sequence: CGTGAGTTTTCGTTCCACTG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          663..682
                     /label="pGL4.23_lin-ori/ampR_F"
                     /note="sequence: AAGGGATTTTGGTCATGAGA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(804..1664)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1505..1524
                     /label="pGL4.23_backbone_4"
                     /note="sequence: TCTTGCCGCTATTGAGATCC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1564..1585)
                     /label="pGL4.23_backbone_3"
                     /note="sequence: CGCTGGTGAAAGTAAAAGATGC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1709..1733
                     /label="p2E-HDR_insertcheck_F"
                     /note="sequence: AGTACGTCTCTCAAGGATAAGTAAG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(1745..1764)
                     /label="pGL4_backbone_R"
                     /note="sequence: CCTGTCCAATACCTCCCGTA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1758..1777)
                     /label="Validation_gblock_bb_lin_R"
                     /note="sequence: TATTTTATTGCGGCCTGTCC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      1769..1817
                     /label="synth_PA_terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      1769..1922
                     /label="up_PA_terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1890..1930
                     /label="Valdation_Insert_ext_F"
                     /note="sequence: CCAGTGCAAGTGCAGGTGCCAGAACATTTCTCTGGCCTAAC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(1898..1917)
                     /label="Valdation_backbon_lin2_F"
                     /note="sequence: AATGTTCTGGCACCTGCACT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1898..1919
                     /label="pGL_MCS_pLB_F"
                     /note="sequence: catttctgccagggcccgctctagaGTGCAGGTGCCAGAACATTTC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1898..1922)
                     /label="Valdation_backbone_MPRAv3_r_R"
                     /note="sequence: CACTGCGGCTCCTGCGATAGAGAAATGTTCTGGCACCTGCACT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1898..1936)
                     /label="Valdation_backbone_MPRAv3_f_R"
                     /note="sequence: CGTCAAGCGGCCAGTTAGGCCAGAGAAATGTTCTGGCACCTGCACT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1907..1931
                     /label="MPRA_Amp2Sc_F"
                     /note="sequence: GCCAGAACATTTCTCTGGCCTAACT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1907..1931)
                     /label="MPRA_Amp2Sc_Rev_F"
                     /note="sequence: AGTTAGGCCAGAGAAATGTTCTGGC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1909..1930)
                     /label="pGL4.23_sfi_lin_2_R"
                     /note="sequence: GTTAGGCCAGAGAAATGTTCTG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(1917..1935)
                     /label="pGL4.23_sfi_lin_1_R"
                     /note="sequence: GGCCAGTTAGGCCAGAGAA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1926..1945
                     /label="MPRA_v3_GFP_Fusion_F"
                     /note="sequence: CACTGCGGCTCCTGCGATCTAACTGGCCGGTACCTGAG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1926..1945
                     /label="Valdation_backbone_MPRAv3_r_F"
                     /note="sequence: CGTCAAGCGGCCAGTTAGGCTAACTGGCCGGTACCTGAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1926..1945
                     /label="Valdation_backbone_MPRAv3_f_F"
                     /note="sequence: CACTGCGGCTCCTGCGATCTAACTGGCCGGTACCTGAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1926..1952
                     /label="sMPRA_PonA_GFP_fusion_F"
                     /note="sequence: ccctggccgacctggtttctaactggccggtacctgagctcgcta"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1926..1952
                     /label="MPRA_v3_GFP_Fusion_v2_F"
                     /note="sequence: CACTGCGGCTCCTGCGATCTAACTGGCCGGTACCTGAGCTCGCTA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1926..1952
                     /label="sMPRA_AonP_GFP_fusion_F"
                     /note="sequence: CACTGCGGCTCCTGCGATctaactggccggtacctgagctcgcta"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1963..1991)
                     /label="pGL4.23_xbaRemoval_R"
                     /note="sequence: GCCGGCCGCCCCGACTAGCTTGGCCGCCGAGGCCAGATCTTGATA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1969..1991)
                     /label="pGL4.23dlucdxba_gibson_R"
                     /note="sequence: GAAGCGGCCGGCCGCCCCGACTAGCTTGGCCGCCGAGGCCAGATC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1973..1992)
                     /label="pGL_leader_only_1"
                     /note="sequence: GGTGGCTTTACCAACAGTACCGGATTGCCAAGCTTGGCCGCCGAGGCCA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1973..2019
                     /label="pGL_minP_leader_F"
                     /note="sequence: TGGCCTCGGCGGCCAAGCTTAGACACTAGAGGGTATATAATGGAAGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          1974..1988
                     /label="pGL4.23_sfi_lin_1_F"
                     /note="sequence: GGCCTCGGCGGCCAA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(1978..1992)
                     /label="pGL4.23_gfp_promoter_linearize_R"
                     /note="sequence: AAGCTTGGCCGCCGA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1979..1991)
                     /label="GFP_3prime_Bio_Probe"
                     /note="sequence: CCGACTAGCTTGGCCGCCG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1979..1993
                     /label="pGL4.23_sfi_lin_2_F"
                     /note="sequence: CGGCGGCCAAGCTTA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(1981..2024)
                     /label="Valdation_Insert_ext_R"
                     /note="sequence: GTCGAGCTTCCATTATATACCCTCTAGTGTCTAAGCTTGGCCGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1985..2011)
                     /label="pGL_4.23_LinBackbone_R"
                     /note="sequence: TATATACCCTCTAGTGTCTAAGCTTGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1992..2019
                     /label="pGL_minP_leader_R"
                     /note="sequence: TCCTCGCCCTTGCTCACCATAGACACTAGAGGGTATATAATGGAAGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        1993..2033
                     /label="minP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          1994..2018
                     /label="Valdation_backbon_lin2_R"
                     /note="sequence: GACACTAGAGGGTATATAATGGAAG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2029..2062
                     /label="pGL_leader_only_1_redo"
                     /note="sequence: TGGCCTCGGCGGCCAAGCTTGGCAATCCGGTACTGTTGGTAAAGCCACC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2034..2081)
                     /label="pGL_leader_only_2"
                     /note="sequence: CCTCGCCCTTGCTCACCATGGTGGCTTTACCAACAGTACCGGATTGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2039..2058
                     /label="Validation_gblock_bb_lin_F"
                     /note="sequence: TCCGGTACTGTTGGTAAAGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2042..2082)
                     /label="pGL_SV40-P_R"
                     /note="sequence: TCCTCGCCCTTGCTCACCATGGTGGCTTTACCAACAGTACC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2042..2082)
                     /label="pGL_minP_leader_R_reorder"
                     /note="sequence: TCCTCGCCCTTGCTCACCATGGTGGCTTTACCAACAGTACC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          2063..2079
                     /label="pGL4.23_gfp_promoter_linearize_F"
                     /note="sequence: ATGGTGAGCAAGGGCGA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2063..2082
                     /label="tdTomato-F"
                     /note="sequence: ATGGTGAGCAAGGGCGAGGA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2063..2082)
                     /label="GFP-Start-R"
                     /note="sequence: TCCTCGCCCTTGCTCACCAT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2063..2088
                     /label="emGFP_pGL4.23_F"
                     /note="sequence: TAGACACTAGAGGGTATATAATGGTGAGCAAGGGCGAGGAGCTGTT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     Gene            2063..2788
                     /label="emGFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2066..2083
                     /label="GFP_qPCR_1_F"
                     /note="sequence: GTGAGCAAGGGCGAGGAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2123..2182)
                     /label="GFP_MPRA_Probe11"
                     /note="sequence: GTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2130..2149)
                     /label="GFP_qPCR_1_R"
                     /note="sequence: GCTGAACTTGTGGCCGTTTA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(2131..2149)
                     /label="GFP_Internal_InsertCheck_R"
                     /note="sequence: GCTGAACTTGTGGCCGTTT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2189..2209
                     /label="GFP_qPCR_2_F"
                     /note="sequence: CTGACCCTGAAGTTCATCTGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2198..2232)
                     /label="GFP_Capture_12"
                     /note="sequence: GGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2233..2267)
                     /label="GFP_Capture_11b"
                     /note="sequence: CGCCGTAGGTGAAGGTGGTCACGAGGGTGGGCCAG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2243..2302)
                     /label="GFP_MPRA_Probe9"
                     /note="sequence: CTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCGTAGGTGAAGGTGGTCACGAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2268..2302)
                     /label="GFP_Capture_10"
                     /note="sequence: CTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2284..2303
                     /label="pX_Validation_4_F"
                     /note="sequence: CTACCCCGACCACATGAAGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(2294..2313)
                     /label="GFP_qPCR_2_R"
                     /note="sequence: AAGTCGTGCTGCTTCATGTG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2347..2366)
                     /label="pX_Validation_3_R"
                     /note="sequence: TGAAGAAGATGGTGCGCTCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2363..2422)
                     /label="GFP_MPRA_Probe7"
                     /note="sequence: CAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2408..2442)
                     /label="GFP_Capture_6"
                     /note="sequence: TTCAGCTCGATGCGGTTCACCAGGGTGTCGCCCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2443..2477)
                     /label="GFP_Capture_5b"
                     /note="sequence: CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2478..2512)
                     /label="GFP_Capture_4"
                     /note="sequence: CTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2483..2542)
                     /label="GFP_MPRA_Probe5"
                     /note="sequence: GTTCTTCTGCTTGTCGGCGGTGATATAGACCTTGTGGCTGTTGTAGTTGTACTCCAGCTT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(2513..2547)
                     /label="GFP_Capture_3"
                     /note="sequence: ATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGAC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2548..2582)
                     /label="GFP_Capture_2b"
                     /note="sequence: CCTCGATGTTGTGGCGGGTCTTGAAGTTCACCTTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2583..2617)
                     /label="GFP_Capture_1"
                     /note="sequence: CTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2603..2662)
                     /label="GFP_MPRA_Probe3"
                     /note="sequence: GTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2680..2696
                     /label="TruSeq_Multiplex_Tag534_GFP"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATATCCGCAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2680..2696
                     /label="TruSeq_Multiplex_Tag880_GFP"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATTCGCCAGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2680..2696
                     /label="TruSeq_Multiplex_Tag823_GFP"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATGATAACCTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2680..2696
                     /label="MPRA_Illumina_GFP_F"
                     /note="sequence: GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2680..2696
                     /label="TruSeq_Multiplex_Tag778_GFP"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATTGTGCTTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2680..2696
                     /label="TruSeq_Multiplex_Tag559_GFP"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATCTAGGTTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2680..2696
                     /label="TruSeq_Multiplex_Tag117_GFP"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATCAGTTGGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGCCCTGAGCAAAGACC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2683..2701
                     /label="GFP_qPCR_3_F"
                     /note="sequence: CCTGAGCAAAGACCCCAAC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(2723..2782)
                     /label="GFP_MPRA_Probe1"
                     /note="sequence: TTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2759..2781)
                     /label="tdTomato-R"
                     /note="sequence: CTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2760..2779)
                     /label="GFP_qPCR_3_R"
                     /note="sequence: CTTGTACAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2760..2792)
                     /label="sMPRA_PonA_GFP_fusion_R"
                     /note="sequence: agcggctgcagcaggtttATTATTATCATTACTTGTACAGCTCgtccatgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2760..2792)
                     /label="MPRA_v3_GFP_Fusion_v2_R"
                     /note="sequence: TCTAGAGGTTCGTCGACGCGATTATTATCATTACTTGTACAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(2760..2792)
                     /label="sMPRA_AonP_GFP_fusion_R"
                     /note="sequence: GAGGTTCGTCGACGCGatATTATTATCATTACTTGTACAGCTCgtccatgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2760..2792)
                     /label="sMPRA_PonA_GFP_fusion_R"
                     /note="sequence: agcggctacagcacacgtttTCTAGAGGTTCGTCGACGCGATTATTATCATTACTTGTACAGCTCgtccatgc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2760..2803
                     /label="emGFP_pGL4.23_R"
                     /note="sequence: GCATGGACGAGCTGTACAAGTAATGATAATAATTCTAGAGTCGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2760..2803)
                     /label="v2_emGFP_pGL4.23_R"
                     /note="sequence: CCGACTCTAGAATTATTATCATTACTTGTACAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2768..2792)
                     /label="MPRA_v3_GFP_Fusion_R"
                     /note="sequence: TCTAGAGGTTCGTCGACGCGATTATTATCATTACTTGTACAGCTC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2786..2806
                     /label="pGL_4.23_LinBackbone_v2_F"
                     /note="sequence: TAATAATTCTAGAGTCGGGGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2786..2809
                     /label="pGL_luc_GFP_F"
                     /note="sequence: GGCATGGACGAGCTGTACAAGtaataattctagagtcggggcggc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2786..2809
                     /label="pGL_luc_PEST_F"
                     /note="sequence: GCTTCTGCTAGGATCAATGTGTAGtaataattctagagtcggggcggc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2798..2819
                     /label="pGL4.23dlucdxba_gibson_F"
                     /note="sequence: GATCTGGCCTCGGCGGCCAAGCTAGTCGGGGCGGCCGGCCGCTTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2798..2831
                     /label="pGL4.23_xbaRemoval_F"
                     /note="sequence: CGGCCAAGCTAGTCGGGGCGGCCGGCCGCTTCGAGCAGACATGA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2803..2804
                     /label="Geneious type: Editing History Deletion"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(2829..2948)
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2852..2873)
                     /label="pGL4.23_InsertCheck_R"
                     /note="sequence: GCATTCTAGTTGTGGTTTGTCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3028..3051)
                     /label="pGL4.23_SV40E_lin2_R"
                     /note="sequence: TCTTTCCGCCTCAGAAGGTTCGATTTTACCACATTTGTAGAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3067..3084)
                     /label="pGL4.23_backbone_5"
                     /note="sequence: AGGCTCTCAAGGGCATCG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3079..3098
                     /label="pGL4.23_SV40E_lin2_F"
                     /note="sequence: AGTTCCGCCCATTCTCCGAGCCTTCAACCCAGTCAGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(3124..3145)
                     /label="pGL4.23_SV40E_lin3_R"
                     /note="sequence: TCTTTCCGCCTCAGAAGGTCATAAGTGCGGCGACGATAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          3158..3177
                     /label="pGL4.23_SV40E_lin3_F"
                     /note="sequence: AGTTCCGCCCATTCTCCGCATGCAACTCGTAGGACAGG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          3160..3179
                     /label="pGL4_backbone_F"
                     /note="sequence: TGCAACTCGTAGGACAGGTG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3188..3205)
                     /label="p2E-HDR_insertcheck_R"
                     /note="sequence: GCGAGGAAGCGGAAGAGC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          3316..3334
                     /label="pUC19_R_pXFusion"
                     /note="sequence: CCGTAAGTTATGTAACGGGTACCTGAGCAAAAGGCCAGCAAA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
       61 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg
      781 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg
      841 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt
      901 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg
      961 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct
     1021 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt
     1081 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc
     1141 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga
     1201 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt
     1261 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc
     1321 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag
     1381 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca
     1441 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca
     1501 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct
     1561 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc
     1621 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa
     1681 tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt
     1741 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg
     1801 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     1861 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     1921 ctggcctaac tggccggtac ctgagctcgc tagcctcgag gatatcaaga tctggcctcg
     1981 gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc
     2041 cggtactgtt ggtaaagcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2101 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2161 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2221 gctgcccgtg ccctggccca ccctcgtgac caccttGacc tacggcgtgc agtgcttcgc
     2281 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2341 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2401 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2461 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
     2521 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
     2581 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2641 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2701 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2761 catggacgag ctgtacaagt aatgataata attctagagt cggggcggcc ggccgcttcg
     2821 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2881 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2941 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     3001 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     3061 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     3121 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     3181 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3241 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggc
//