LOCUS       Exported                5061 bp ds-DNA     circular SYN 15-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 15, 2018 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
COMMENT     New DNA from 1 to 2686
            seq0001b_pUC19a from 1 to 2686
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             399..1058
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1156..1838
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    2178..2200
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     2206..2226
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(4667..4684)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             4755..4823
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcctgc aggtaactca tccttgtcag catcgtttcc
     2281 tcacctcctc ctcctgtcgc tggtgcttct ggtcttgtcc agcgggaacc caaacaacaa
     2341 caacaacgaa ctaccactgc ctcggaaaat ctgcggtgat ttgcacaaat cgccaacaag
     2401 tgttgtggcg tgctaaatga gagaaacacc gtatttgagc ccaggtatcg aaatgtctgt
     2461 atggctgcat ttgtgttttc caacctgaag gaaaacagca cttgacaacg acgtttgccc
     2521 agcccgtttt tcgcggtgga tacaaatttc acctgtgtca ctgcgcatta gccccacagt
     2581 ggggcttacg aaatctacac catcccagct tccgcaccgc caaaattaac tgcgtgaaaa
     2641 aacacttgcg gcaaccacag caaccaccac aagcagccaa acactcgcaa acacccttgc
     2701 ttgcttcttg gcttttttgt agtccccttt cccttgtttt cctgttgtcg tggctgaggt
     2761 ttgcgtggcg tgttcatttt catcagtttg ctcgctcata acaagcttgc gactacctcc
     2821 cctttttggt gctgtacacc cgcgaccttt cttatcctct tctccttctc caccaccgct
     2881 caacgacaag atgcgcgaag ttctctccgt ccacattggc caggccggtt gccagatcgg
     2941 taacgcctgc tgggagctgt actgcctcga gcacggcatc caacccgatg gccagatgcc
     3001 ctctgacaag actgtcggct atggcgacga ctcgttcaac accttcttct ccgagactgg
     3061 tgctggcaag cacgtgcccc gcgccgtgtt tgtcgatctc gagcccaccg tcgtcgacga
     3121 ggtgcgcacc ggcacctacc gccagctgtt ccaccccgag cagctcatct ccggcaagga
     3181 ggacgccgcc aacaactacg cccgtggcca ctacaccgtt ggcaaggagc tcgtcgacat
     3241 tgtcctcgac cgcatccgca agctcgccga ccagtgcacc ggtctccagg gcttcctcat
     3301 cttccactcg ttcggtggcg gtaccggctc tggctttgcc tcgctcctca tggagcgcct
     3361 ctctgtcgac tacggcaaga agtccaagct cgagtttgcc gtctaccctg ctcctcaggt
     3421 gtccaccgcc gttgtcgagc cctacaactc gatcctgacg tgccacacca ccctcgagca
     3481 ctctgactgc gccttcctca tggacaacga ggccatctac gacatttgcc gtcgcaacct
     3541 cgacattgag cgccccacct acaccaacct gaaccgcctc atcgcccagg ttgtgtcctc
     3601 cgtgacggcc tccctgcgct tcgacggtgc ccttaacgtc gatctcaccg agttccagac
     3661 caacctggtg ccctaccccc gcatccactt ccctctggtg acgtacgctc ccatcatctc
     3721 tgccgagaag gcctaccacg agcagctctc cgttgccgag atcaccaact cgtgcttcga
     3781 gcccgccaac cagatggtca agtgcgaccc ccgccacggc aagtacatgg cctgctgcgt
     3841 cctctaccgc ggtgacgtcg tccccaagga cgtcaacgcc tcgattgcca ccatcaagac
     3901 caagcgcacc atccagtttg tcgactggtg ccccactggc ttcaaggttg gcatcaacta
     3961 ccagccaccc accgtcgtgc ccggcggcga ccttgccaag gtgcagcgct ccgtctgcat
     4021 gctcagcaac accaccgcca ttgccgaggc ctgggctcgt cttgaccaca agttcgacct
     4081 catgtacgcc aagcgtgcct tcgtccactg gtacgtcggt gagggtatgg aggaaggcga
     4141 gttcgccgag gcccgcgagg atcttgccgc cctcgagaag gattacgagg aggtcggcgt
     4201 cgactccatg gacggcgagg gcgaggacga gggcgaggag tactaagcgc aaggccccct
     4261 cccggcctga cctccaccac caccgtcttc actgctgcaa ttacctgcca ttgttttggc
     4321 tgtcctttga tgactggttg atgcttctgc atttgttttg tgtgtgttga gctgtctggt
     4381 ggtggcgcgt ttgttcactc ttccacccct tgcctttgct gcaagcgacc gttccatgtc
     4441 cccaaccatc caatacaaac actgaatcca acaacaattg cagcaaagag gaataagttg
     4501 tccaacagaa cgaagttatg atgagtgttg tgtgggatgt cacacatgac gcgacactgg
     4561 cagtggctgt tttgtttggt tggtggttta ctggtccgct gcgtggcggt agcgatcata
     4621 gaaggcaagc atgtctagag gatccccggg taccgagctc gaattcactg gccgtcgttt
     4681 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4741 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4801 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     4861 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4921 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4981 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5041 cgtcatcacc gaaacgcgcg a
//
