LOCUS       Exported                4224 bp ds-DNA     circular SYN 15-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 15, 2018 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             399..1058
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1156..1838
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    2178..2200
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     2206..2226
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    2254..2890
                     /locus_tag="New Feature"
                     /label=New Feature
     misc_feature    2254..2890
                     /locus_tag="pTub"
                     /label=pTub
     misc_feature    2897..3409
                     /locus_tag="NanoLuc_SrosettaOpt"
                     /label=NanoLuc_SrosettaOpt
     misc_feature    3410..3796
                     /locus_tag="3'tub"
                     /label=3'tub
     primer_bind     complement(3830..3847)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             3918..3986
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcctgc aggtaactca tccttgtcag catcgtttcc
     2281 tcacctcctc ctcctgtcgc tggtgcttct ggtcttgtcc agcgggaacc caaacaacaa
     2341 caacaacgaa ctaccactgc ctcggaaaat ctgcggtgat ttgcacaaat cgccaacaag
     2401 tgttgtggcg tgctaaatga gagaaacacc gtatttgagc ccaggtatcg aaatgtctgt
     2461 atggctgcat ttgtgttttc caacctgaag gaaaacagca cttgacaacg acgtttgccc
     2521 agcccgtttt tcgcggtgga tacaaatttc acctgtgtca ctgcgcatta gccccacagt
     2581 ggggcttacg aaatctacac catcccagct tccgcaccgc caaaattaac tgcgtgaaaa
     2641 aacacttgcg gcaaccacag caaccaccac aagcagccaa acactcgcaa acacccttgc
     2701 ttgcttcttg gcttttttgt agtccccttt cccttgtttt cctgttgtcg tggctgaggt
     2761 ttgcgtggcg tgttcatttt catcagtttg ctcgctcata acaagcttgc gactacctcc
     2821 cctttttggt gctgtacacc cgcgaccttt cttatcctct tctccttctc caccaccgct
     2881 caacgacaag atgcatgtct tcaccctcga ggacttcgtc ggcgactggc gccagaccgc
     2941 tggttacaac ctcgaccagg tcctcgagca gggcggcgtc tcctccctct tccagaacct
     3001 cggcgtctcc gtcaccccca tccagcgcat cgtcctctcc ggcgagaacg gcctcaagat
     3061 cgacatccac gtcatcatcc cctacgaggg cctctccggc gaccagatgg gccagatcga
     3121 gaagatcttc aaggtcgtct accccgtcga cgaccaccac ttcaaggtca tcctccacta
     3181 cggcaccctc gtcatcgacg gcgtcacccc caacatgatc gactacttcg gccgccccta
     3241 cgagggcatc gccgtcttcg acggcaagaa gatcaccgtc accggcaccc tctggaacgg
     3301 caacaagatc atcgacgagc gcctcatcaa ccccgacggc tccctcctct tccgcgtcac
     3361 catcaacggc gtcaccggct ggcgcctctg cgagcgcatc ctcgcttaag cgcaaggccc
     3421 cctcccggcc tgacctccac caccaccgtc ttcactgctg caattacctg ccattgtttt
     3481 ggctgtcctt tgatgactgg ttgatgcttc tgcatttgtt ttgtgtgtgt tgagctgtct
     3541 ggtggtggcg cgtttgttca ctcttccacc ccttgccttt gctgcaagcg accgttccat
     3601 gtccccaacc atccaataca aacactgaat ccaacaacaa ttgcagcaaa gaggaataag
     3661 ttgtccaaca gaacgaagtt atgatgagtg ttgtgtggga tgtcacacat gacgcgacac
     3721 tggcagtggc tgttttgttt ggttggtggt ttactggtcc gctgcgtggc ggtagcgatc
     3781 atagaaggca agcatgtcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
     3841 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     3901 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     3961 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     4021 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4081 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4141 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4201 caccgtcatc accgaaacgc gcga
//
