LOCUS       Exported                4601 bp ds-DNA     circular SYN 14-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 14, 2018 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             399..1058
                     /label=AmpR
     rep_origin      1156..1838
                     /label=ColE1 origin
     misc_binding    2178..2200
                     /label=LacO
     primer_bind     2206..2226
                     /label=M13-rev
     misc_feature    2235..2829
                     /label=PTSG_10057_H3locus
     misc_feature    3367..4179
                     /label=PTSG_10057_H3locus
                     /label=PTSG_10057_H3locus(1)
     primer_bind     complement(4207..4224)
                     /label=M13-fwd
     CDS             4295..4363
                     /label=LacZ alpha
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gtaagtagtg gtagtcgtcg aagacgtggg gtgcggggat
     2281 gtgcttgact tggtgggaga ggctggtgga agtagctgat gaaaatgaac tccaccacac
     2341 gccaactgcc acttgaacca cggaaggcag gcagcagtgc tgctgtgtgc gcttttcggc
     2401 gcggcgcgca gccacaaagc aacggcaatt gtggcgggtg attcgttcga acgctgcttt
     2461 tcataatttt cgccaatgtc tgaatcaatg tctttttcaa gggtgcagaa taacgaacat
     2521 tattttttat tgtgtggatt cagcgccaat tgcgatttgg agatgcgcta agcgcacaat
     2581 caccaccaaa cacgacattc cccgctttcc cgccaaactg attctcgcgc gaaacctgag
     2641 atgtgcaccg cgccgttaca agtgcgacca cgcacacctc ttcttgtcat ttatcatccg
     2701 catctccaag caagcaagca agcaaaccag gaccccctcc ctcgctcgtc acctcaccac
     2761 ctcccaaact taaacttcac aaccagcaca atggcccgca cgaagcagac cgcccgcaag
     2821 accaccggtt ctggtggctc tggtggcatg gtcttcaccc tcgaggactt cgtcggcgac
     2881 tggcgccaga ccgccggcta caacctcgac caggtcctcg agcagggcgg cgtctcctcc
     2941 ctcttccaga acctcggcgt ctccgtcacc cccatccagc gcatcgtcct ctccggcgag
     3001 aacggcctca agatcgacat ccacgtcatc atcccctacg agggcctctc cggcgaccag
     3061 atgggccaga tcgagaagat cttcaaggtc gtctaccccg tcgacgacca ccacttcaag
     3121 gtcatcctcc actacggcac cctcgtcatc gacggcgtca cccccaacat gatcgactac
     3181 ttcggccgcc cctacgaggg catcgccgtc ttcgacggca agaagatcac cgtcaccggc
     3241 accctctgga acggcaacaa gatcatcgac gagcgcctca tcaaccccga cggctccctc
     3301 ctcttccgcg tcaccatcaa cggcgtcacc ggctggcgcc tctgcgagcg catcctcgcc
     3361 gcttaagcgt ctcaccttct caacatggct cgctcgctcg atgtcgttct ctgacacctt
     3421 gcttggcttg tcgtcatgag gttgctttgt catcaccaac gctggttgca ttttgatctg
     3481 tgacaccttg aatcatcctc cctcccctcc cttgttctta attacccact tggcccttgc
     3541 tgtctctctt ggtctgtgcc acgggttgtc gcctccgcgg gctcaaattg tgctcacacg
     3601 tcttttatcc atgctcgtgt tcctgagcac ttgacgctgg cggtgtcttt gtgcgctcgt
     3661 gtgcatggga acctctctcc ctccctctcc ctgtgtgtaa tggcgttggc ttaccgtggt
     3721 atcgatttgc ctctgcgtaa tgacacgccg caacggcgtt tggggagttt ccccggcttg
     3781 tcctgcctcg caacccttct cctttggttc gttgttgcct ctcaatacaa tgatcaaacc
     3841 gaacattaat gtgaggacca tgatggcatc ctgctttatt caagcaagtg gaatgaatga
     3901 cacagaaaca gattgatcgt cattgtgatg ccgtgcgtgt atttgtggcg gagaggaaca
     3961 aaagtcacag gtggctacat ttacacaact tcttgtcaat gcaaactcag tgagtcattg
     4021 ggtatcatgc gtggtgaaga atgagggagt gctgccagca tctgaacgag caccatcgtg
     4081 ccagggcgcg gctcttggca aactcgcgtg cgtccatctc ttcgtgatca tcgtcatcat
     4141 cgcgacgctg tgtcacatgt gggacaatga atgtctagag gatccccggg taccgagctc
     4201 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4261 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4321 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     4381 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     4441 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4501 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4561 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
