LOCUS       Exported                5165 bp ds-DNA     circular SYN 15-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 15, 2018 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             399..1058
                     /label=AmpR
     rep_origin      1156..1838
                     /label=ColE1 origin
     misc_binding    2178..2200
                     /label=LacO
     primer_bind     2206..2226
                     /label=M13-rev
     misc_feature    2235..3190
                     /label=PTSG_10057_H3locus
     misc_feature    3932..4743
                     /label=PTSG_10057_H3locus
                     /label=PTSG_10057_H3locus(1)
     primer_bind     complement(4771..4788)
                     /label=M13-fwd
     CDS             4859..4927
                     /label=LacZ alpha
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gtaagtagtg gtagtcgtcg aagacgtggg gtgcggggat
     2281 gtgcttgact tggtgggaga ggctggtgga agtagctgat gaaaatgaac tccaccacac
     2341 gccaactgcc acttgaacca cggaaggcag gcagcagtgc tgctgtgtgc gcttttcggc
     2401 gcggcgcgca gccacaaagc aacggcaatt gtggcgggtg attcgttcga acgctgcttt
     2461 tcataatttt cgccaatgtc tgaatcaatg tctttttcaa gggtgcagaa taacgaacat
     2521 tattttttat tgtgtggatt cagcgccaat tgcgatttgg agatgcgcta agcgcacaat
     2581 caccaccaaa cacgacattc cccgctttcc cgccaaactg attctcgcgc gaaacctgag
     2641 atgtgcaccg cgccgttaca agtgcgacca cgcacacctc ttcttgtcat ttatcatccg
     2701 catctccaag caagcaagca agcaaaccag gaccccctcc ctcgctcgtc acctcaccac
     2761 ctcccaaact taaacttcac aaccagcaca atggcccgca cgaagcagac cgcccgcaag
     2821 accaccggtg gcaaggcccc ccgcaagcag ctcgccacca aggctgcccg caagtccgcc
     2881 ccctccactg gcggcgtgaa gaagccccat cgcttccgcc ccggtaccgt cgctctgcgt
     2941 gagatccgcc gctaccagaa gtccaccgag ctgctgatcc gcaagctccc cttccagcgc
     3001 ctcgtccgcg agattgccca ggacttcaag accgacctcc gcttccagtc caccgccgtc
     3061 tctgccctgc aggaggccgc cgaggcttac ctcgtcaacc tctttgagga caccaacctc
     3121 tgcgccatcc acgcaaagcg cgtcaccatc atgcccaagg acatccagct ggcccgccgc
     3181 atccgtggcg agcgctctgg cggtagcggt ggctccatgg tctccaaggg cgaggaggac
     3241 aacatggcta tcatcaagga gttcatgcgc ttcaaggtcc acatggaggg ctccgtcaac
     3301 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3361 gccaagctca aggtgacgaa gggcggcccc ctccccttcg cctgggacat cctctccccc
     3421 cagttcatgt acggctccaa ggcttacgtc aagcaccccg ccgacatccc cgactacctc
     3481 aagctctcct tccccgaggg cttcaagtgg gagcgcgtca tgaacttcga ggacggcggc
     3541 gtcgtcaccg tcacccagga ctcctctctt caggacggcg agttcatcta caaggtcaag
     3601 ctgcgcggca ccaacttccc ctccgacggc cccgtcatgc agaagaagac gatgggttgg
     3661 gaggcttcct ccgagcgcat gtaccccgag gacggcgccc tcaagggcga gatcaagcag
     3721 cgcctcaagc tcaaggacgg cggccactac gacgccgagg tcaagaccac ctacaaggcc
     3781 aagaagcccg tccagctccc cggcgcctac aacgtcaaca tcaagctcga catcacctcc
     3841 cacaacgagg actacaccat cgtcgagcag tacgagcgtg ccgagggccg ccacagcacg
     3901 ggcggtatgg acgagcttta caagtcttaa gcgtctcacc ttctcaacat ggctcgctcg
     3961 ctcgatgtcg ttctctgaca ccttgcttgg cttgtcgtca tgaggttgct ttgtcatcac
     4021 caacgctggt tgcattttga tctgtgacac cttgaatcat cctccctccc ctcccttgtt
     4081 cttaattacc cacttggccc ttgctgtctc tcttggtctg tgccacgggt tgtcgcctcc
     4141 gcgggctcaa attgtgctca cacgtctttt atccatgctc gtgttcctga gcacttgacg
     4201 ctggcggtgt ctttgtgcgc tcgtgtgcat gggaacctct ctccctccct ctccctgtgt
     4261 gtaatggcgt tggcttaccg tggtatcgat ttgcctctgc gtaatgacac gccgcaacgg
     4321 cgtttgggga gtttccccgg cttgtcctgc ctcgcaaccc ttctcctttg gttcgttgtt
     4381 gcctctcaat acaatgatca aaccgaacat taatgtgagg accatgatgg catcctgctt
     4441 tattcaagca agtggaatga atgacacaga aacagattga tcgtcattgt gatgccgtgc
     4501 gtgtatttgt ggcggagagg aacaaaagtc acaggtggct acatttacac aacttcttgt
     4561 caatgcaaac tcagtgagtc attgggtatc atgcgtggtg aagaatgagg gagtgctgcc
     4621 agcatctgaa cgagcaccat cgtgccaggg cgcggctctt ggcaaactcg cgtgcgtcca
     4681 tctcttcgtg atcatcgtca tcatcgcgac gctgtgtcac atgtgggaca atgaatgtct
     4741 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     4801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4921 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4981 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5041 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5101 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5161 cgcga
//
