LOCUS       Exported                5932 bp ds-DNA     circular SYN 15-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 15, 2018 from SnapGene Viewer 4.1.6
            http://www.snapgene.com
COMMENT     pDSB00ZZZ_pEFL_mTFP_PTSG_07215_3act from 1 to 5914
            /Users/dsboothacosta/Desktop/PlasmidSequences/pDSB0093_pEFL_mTFP1_3 
            utr.ape from 1 to 4678
            /Users/dsboothacosta/Desktop/PlasmidSequences/pDSB0051_PTSG_02256_n 
            anoluc.ape from 1 to 4645
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             399..1058
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1156..1838
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    2178..2200
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     2206..2226
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(5538..5555)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             5626..5694
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatc ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gtaacgactg cagtcttgtt gtcgtttgtc gtcgtcgttc
     2281 tctgtctccc acccaacttt tgccaattgc aacagtgact tctgtacaat ttgcaacaga
     2341 taccaacgtg tcgagtcagc acaaatggcg aggaaggttc gcgtgcattg cttgcgcgcc
     2401 tctttgtcat gggcgcacgc tgatttgtgt gtgatttgca ccaaatttcc tgccgacaca
     2461 acttgggccc gtaccacacg acccgtttct ggacccgttt ctggcccaga aacgcgaatg
     2521 ttgcgaaaga tgcgaccacg tgctcgctgt cgacaacaca aactcccgca caatccccat
     2581 gaaaaaaaaa gtgcttgctg ggcttgtgtc tggcctgtgt gcgtggcgtg gccgcgtgtg
     2641 gcttcgttct tgcttctgct cactcagcct gttacacgca gagtggctgc gagaaacaag
     2701 gagaggggtt tgcggcattg gcgcatgtgc atctgccttt ttcattgagt cagtctgcag
     2761 ctcaagaggt tggtttcgtc gacggatatg agtgagcttc tatggatgat gagaggtggt
     2821 ggtggttgta tggttggtgg ttggtggttg gtgcaaggcg agccacgcga agagcgacaa
     2881 ggatgaggga gaggagagga gactaggaaa gaggtgaagg gaagggaagg gcgagcaatg
     2941 acgacgagat aaaagacagc caccaccgca cccgcacaaa agcattgctc gcacgcatgg
     3001 tcgtgtgtta tgtttcacac caccaacata accaatacaa gccatcacca atacaagcca
     3061 gcgctcaagc acacacgcac gcacgcacac acaatacaca cgcacgtgaa acgttcacat
     3121 tgtccgctca caaatgcctt tcccttctcg tcgctttcct tgcctttttt tcttcccctc
     3181 ctgacttcct tcctgccttc cttccttcct ccccttctcc gtccaccaac accatttatt
     3241 tctctgctct ttttcctcgt gccacgtgag cagtcttttc gcgtcgtctt cttctttgac
     3301 cccgtttacc gtcaacacca tcaacaacca gcaacatggt ctccaagggc gaggagacca
     3361 cgatgggcgt catcaagccc gacatgaaga tcaagctcaa gatggagggc aacgtcaacg
     3421 gccacgcctt cgtcatcgag ggcgagggcg agggcaagcc ctacgacggc accaacacca
     3481 tcaacctcga ggtcaaggag ggcgcccccc tccccttctc ctacgacatc ctcaccaccg
     3541 ccttcgccta cggcaaccgc gccttcacca agtaccccga cgacatcccc aactacttca
     3601 agcagtcctt ccccgagggc tactcctggg agcgcaccat gaccttcgag gacaagggca
     3661 tcgtcaaggt caagtccgac atctctatgg aggaggactc cttcatctac gagatccacc
     3721 tcaagggcga gaacttcccc cccaacggcc ccgtcatgca gaagaagacc accggctggg
     3781 acgcctccac cgagcgcatg tacgtccgcg acggcgtcct caagggcgac gtgaagcaca
     3841 agctcctcct cgagggcggc ggccaccacc gcgtcgactt caagaccatc taccgcgcca
     3901 agaaggccgt caagctcccc gactaccact tcgtcgacca ccgcatcgag atcctcaacc
     3961 acgacaagga ctacaacaag gtcaccgtct acgagtccgc cgtcgcccgc aactccaccg
     4021 acggcatgga cgagctgtac aaggcttctg gcggtagcgg tggctccagt ggcgacatag
     4081 gctttgccaa cctccccaat caaatccatc gtgaggtcac gctcaagggg tttgacttca
     4141 ctttgatggt cgttgggcgg tctggattgg gaaaatcgac gtttgtcaac accctgttct
     4201 ccaccgacat ctatgaagag gacaagccct atcccccacc ttgtgctcgc attcccagca
     4261 caacctctgt gcaggagagc gtgacaacgc tcattgagaa cgacgttcag ctcacgctca
     4321 cgctcgtgga cacgccgggc tttggaaacg gcatcaacaa cacagactgc tgggtcccca
     4381 ttgaggagta tgttgacaat gcgttctcca agtacgaggg cgaggagtcg aagatcagcc
     4441 gcacggatat ccgtgattgc cgcgtccact gctgcctcta cttcctcact ccttgcatcc
     4501 acggcgtccg tcccctcgac attgaagtca tgaagcgcct gcacacgaag gtgaacatca
     4561 tccccgtcat tgcaaaggcg gattcgctca cagtggacga gtgccagctc ctcaaggaac
     4621 gcgtgcgcgc cacgctggcg gagcacaaca tcaacgtgtt tgagtttccc gaagatgacg
     4681 atgatgatga agaggtgcaa gacgacgttg caaacgccag gtctgcgtgg ccgtttgctg
     4741 tatcggcctc aacacaggta ctcttcgcag atgataagga aatgcgtggc cgcgcctacc
     4801 cctggggtta cgtcgaagtc gacaatccgc agcactcgga cttcgttggc ctgcgcaacc
     4861 taatcatcag aacgtacatg cagcagctga ttgacacgac gaacaacgtc cactacgagc
     4921 gtttccgtca gcagcgcctc tccagcctct ctcttgacac atccatcagt ggaagcccca
     4981 aatcggatcc caacagccaa aacttcatcg aacagctcga aggacagctc aaacaacacg
     5041 agaagaagct tgtgaagatg gagcgagaga tgacagaagt ctttgagaag cgcgtgcgcg
     5101 aaaaacagca gaaaatcaag acgcaagagg cgcagctgct gaaggagcac gaggagtttg
     5161 aagaaaagtt gaagaagcag caaagggagc ttgagatgca gcgcgagcag tttgagcggg
     5221 agaagaagga gtacgaggcc gaggaggaga agcgaaaggc cgcacaggcg cagtccactg
     5281 gccggcgcaa gaagttcttc taagcgcctc gttggtcatt gccaccttgt tgcctctttt
     5341 gctgttgtcc tcgtgtcgac gcgtgccacg tgcgcttgtt gtgagttgat tctgcttctt
     5401 gtgcacgtca tgccgagccc ttgggccccg caataaaccc actatgccag tgtaactatc
     5461 cttcccccca atgtataact gtgacgatca atgagcggtg cccgtctaga ggatccccgg
     5521 gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5581 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5641 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     5701 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     5761 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5821 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5881 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc ga
//
