LOCUS       pHS15_(hANGPTL3-Strep)_ 6393 bp ds-DNA     circular     18-SEP-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      2625..3053
                     /label="f1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        863..879
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    923..911
                     /label="pCDNA6/V5-His B"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    912..922
                     /label="pMD2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2303..2326
                     /label="Strep Tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3510..3908
                     /label="Bsd (R)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3442..3509
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(6258..6356)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        3058..3427
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(5397..6257)
                     /label="Amp (R)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             923..2302
                     /label="hANGPTL3"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2355..2579
                     /label="BGH polyA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(4579..5252)
                     /label="pUC origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        232..819
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             923..2326
                     /label="hANGPTL3-Strep"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      4066..4196
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccATGTTCAC AATTAAGCTC CTTCTTTTTA TTGTTCCTCT
      961 AGTTATTTCC TCCAGAATTG ATCAAGACAA TTCATCATTT GATTCTCTAT CTCCAGAGCC
     1021 AAAATCAAGA TTTGCTATGT TAGACGATGT AAAAATTTTA GCCAATGGCC TCCTTCAGTT
     1081 GGGACATGGT CTTAAAGACT TTGTCCATAA GACGAAGGGC CAAATTAATG ACATATTTCA
     1141 AAAACTCAAC ATATTTGATC AGTCTTTTTA TGATCTATCG CTGCAAACCA GTGAAATCAA
     1201 AGAAGAAGAA AAGGAACTGA GAAGAACTAC ATATAAACTA CAAGTCAAAA ATGAAGAGGT
     1261 AAAGAATATG TCACTTGAAC TCAACTCAAA ACTTGAAAGC CTCCTAGAAG AAAAAATTCT
     1321 ACTTCAACAA AAAGTGAAAT ATTTAGAAGA GCAACTAACT AACTTAATTC AAAATCAACC
     1381 TGAAACTCCA GAACACCCAG AAGTAACTTC ACTTAAAACT TTTGTAGAAA AACAAGATAA
     1441 TAGCATCAAA GACCTTCTCC AGACCGTGGA AGACCAATAT AAACAATTAA ACCAACAGCA
     1501 TAGTCAAATA AAAGAAATAG AAAATCAGCT CAGAAGGACT AGTATTCAAG AACCCACAGA
     1561 AATTTCTCTA TCTTCCAAGC CAAGAGCACC AAGAACTACT CCCTTTCTTC AGTTGAATGA
     1621 AATAAGAAAT GTAAAACATG ATGGCATTCC TGCTGAATGT ACCACCATTT ATAACAGAGG
     1681 TGAACATACA AGTGGCATGT ATGCCATCAG ACCCAGCAAC TCTCAAGTTT TTCATGTCTA
     1741 CTGTGATGTT ATATCAGGTA GTCCATGGAC ATTAATTCAA CATCGAATAG ATGGATCACA
     1801 AAACTTCAAT GAAACGTGGG AGAACTACAA ATATGGTTTT GGGAGGCTTG ATGGAGAATT
     1861 TTGGTTGGGC CTAGAGAAGA TATACTCCAT AGTGAAGCAA TCTAATTATG TTTTACGAAT
     1921 TGAGTTGGAA GACTGGAAAG ACAACAAACA TTATATTGAA TATTCTTTTT ACTTGGGAAA
     1981 TCACGAAACC AACTATACGC TACATCTAGT TGCGATTACT GGCAATGTCC CCAATGCAAT
     2041 CCCGGAAAAC AAAGATTTGG TGTTTTCTAC TTGGGATCAC AAAGCAAAAG GACACTTCAA
     2101 CTGTCCAGAG GGTTATTCAG GAGGCTGGTG GTGGCATGAT GAGTGTGGAG AAAACAACCT
     2161 AAATGGTAAA TATAACAAAC CAAGAGCAAA ATCTAAGCCA GAGAGGAGAA GAGGATTATC
     2221 TTGGAAGTCT CAAAATGGAA GGTTATACTC TATAAAATCA ACCAAAATGT TGATCCATCC
     2281 AACAGATTCA GAAAGCTTTG AATGGAGCCA CCCGCAGTTC GAAAAAtgag tttaaacccg
     2341 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2401 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2461 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2521 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     2581 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     2641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     2701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2821 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2941 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     3061 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3121 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3181 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3241 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3301 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     3361 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     3421 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     3481 tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca
     3541 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     3601 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     3661 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     3721 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     3781 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     3841 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     3901 agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     3961 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4021 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     4081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     4201 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4261 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4321 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4381 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4441 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5041 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5701 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6001 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6361 cgcacatttc cccgaaaagt gccacctgac gtc
//