LOCUS       109120_AICSDP-43        2732 bp    DNA     linear   UNA 19-DEC-2018
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local.amandah.54-9vz1gtw
VERSION     urn.local.amandah.54-9vz1gtw
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     mRNA            <1..>1000
                     /gene="GJA1"
                     /gene_synonym="AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS;
                     ODDD; PPKCA"
                     /product="gap junction protein alpha 1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /transcript_id="NM_000165.4"
                     /db_xref="GeneID:2697"
                     /db_xref="HGNC:HGNC:4274"
                     /db_xref="HPRD:00414"
                     /db_xref="MIM:121014"
                     /label="GJA1 mRNA"
     gene            <1..>1000
                     /gene="GJA1"
                     /gene_synonym="AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS;
                     ODDD; PPKCA"
                     /note="gap junction protein alpha 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq."
                     /db_xref="GeneID:2697"
                     /db_xref="HGNC:HGNC:4274"
                     /db_xref="HPRD:00414"
                     /db_xref="MIM:121014"
                     /label="GJA1 gene"
     CDS             <2..>1000
                     /gene="GJA1"
                     /gene_synonym="AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS;
                     ODDD; PPKCA"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /codon_start=1
                     /product="gap junction alpha-1 protein"
                     /protein_id="NP_000156.1"
                     /db_xref="CCDS:CCDS5123.1"
                     /db_xref="GeneID:2697"
                     /db_xref="HGNC:HGNC:4274"
                     /db_xref="HPRD:00414"
                     /db_xref="MIM:121014"
                     /translation="MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESA
                     WGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRK
                     EEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISIL
                     FKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSL
                     VSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTA
                     PLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQP
                     FDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI"
                     /label="GJA1 CDS"
     misc_RNA        960..979
                     /created_by="User"
                     /modified_by="User"
                     /label="crRNA4 Binding Site"
     misc_RNA        973..992
                     /created_by="User"
                     /modified_by="User"
                     /label="crRNA1 Binding Site"
     misc_RNA        complement(980..999)
                     /created_by="User"
                     /modified_by="User"
                     /label="crRNA3 Binding Site"
     misc_feature    982
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     modified_base   982
                     /created_by="User"
                     /modified_by="User"
                     /label="G to C"
     misc_feature    985
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     modified_base   985
                     /created_by="User"
                     /modified_by="User"
                     /label="T to A"
     misc_feature    988
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     modified_base   988
                     /created_by="User"
                     /modified_by="User"
                     /label="T to C"
     misc_feature    1001..1018
                     /created_by="User"
                     /modified_by="User"
                     /label="DPPVAT Linker"
     ORF             1019..1732
                     /created_by="User"
                     /modified_by="User"
                     /label="mEGFP"
     CDS             <1733..1735
                     /gene="GJA1"
                     /gene_synonym="AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS;
                     ODDD; PPKCA"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /codon_start=1
                     /product="gap junction alpha-1 protein"
                     /protein_id="NP_000156.1"
                     /db_xref="CCDS:CCDS5123.1"
                     /db_xref="GeneID:2697"
                     /db_xref="HGNC:HGNC:4274"
                     /db_xref="HPRD:00414"
                     /db_xref="MIM:121014"
                     /translation="MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESA
                     WGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRK
                     EEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISIL
                     FKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSL
                     VSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTA
                     PLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQP
                     FDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI"
                     /label="GJA1 CDS"
     mRNA            <1733..>2732
                     /gene="GJA1"
                     /gene_synonym="AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS;
                     ODDD; PPKCA"
                     /product="gap junction protein alpha 1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /transcript_id="NM_000165.4"
                     /db_xref="GeneID:2697"
                     /db_xref="HGNC:HGNC:4274"
                     /db_xref="HPRD:00414"
                     /db_xref="MIM:121014"
                     /label="GJA1 mRNA"
     gene            <1733..>2732
                     /gene="GJA1"
                     /gene_synonym="AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS;
                     ODDD; PPKCA"
                     /note="gap junction protein alpha 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq."
                     /db_xref="GeneID:2697"
                     /db_xref="HGNC:HGNC:4274"
                     /db_xref="HPRD:00414"
                     /db_xref="MIM:121014"
                     /label="GJA1 gene"
     misc_RNA        1752..1771
                     /created_by="User"
                     /modified_by="User"
                     /label="crRNA2 Binding Site"
     misc_feature    1774
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     modified_base   1774
                     /created_by="User"
                     /modified_by="User"
                     /label="G to C"
ORIGIN      
        1 gtctgccttt cgttgtaaca ctcagcaacc tggttgtgaa aatgtctgct atgacaagtc
       61 tttcccaatc tctcatgtgc gcttctgggt cctgcagatc atatttgtgt ctgtacccac
      121 actcttgtac ctggctcatg tgttctatgt gatgcgaaag gaagagaaac tgaacaagaa
      181 agaggaagaa ctcaaggttg cccaaactga tggtgtcaat gtggacatgc acttgaagca
      241 gattgagata aagaagttca agtacggtat tgaagagcat ggtaaggtga aaatgcgagg
      301 ggggttgctg cgaacctaca tcatcagtat cctcttcaag tctatctttg aggtggcctt
      361 cttgctgatc cagtggtaca tctatggatt cagcttgagt gctgtttaca cttgcaaaag
      421 agatccctgc ccacatcagg tggactgttt cctctctcgc cccacggaga aaaccatctt
      481 catcatcttc atgctggtgg tgtccttggt gtccctggcc ttgaatatca ttgaactctt
      541 ctatgttttc ttcaagggcg ttaaggatcg ggttaaggga aagagcgacc cttaccatgc
      601 gaccagtggt gcgctgagcc ctgccaaaga ctgtgggtct caaaaatatg cttatttcaa
      661 tggctgctcc tcaccaaccg ctcccctctc gcctatgtct cctcctgggt acaagctggt
      721 tactggcgac agaaacaatt cttcttgccg caattacaac aagcaagcaa gtgagcaaaa
      781 ctgggctaat tacagtgcag aacaaaatcg aatggggcag gcgggaagca ccatctctaa
      841 ctcccatgca cagccttttg atttccccga tgataaccag aattctaaaa aactagctgc
      901 tggacatgaa ttacagccac tagccattgt ggaccagcga ccttcaagca gagccagcag
      961 tcgtgccagc agcagacctc gcccagacga cctggagatc gaccctcctg tggccacagt
     1021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1081 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1201 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1441 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1501 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1621 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagataca
     1741 ggcttgaaag catcaagatt ccactcaatt gtgcagaaga aaaaaggtgc tgtagaaagt
     1801 gcaccaggtg ttaattttga tccggtggag gtggtactca acagccttat tcatgaggct
     1861 tagaaaacac aaagacatta gaatacctag gttcactggg ggtgtatggg gtagatgggt
     1921 ggagagggag gggataagag aggtgcatgt tggtatttaa agtagtggat tcaaagaact
     1981 tagattataa ataagagttc cattaggtga tacatagata agggcttttt ctccccgcaa
     2041 acacccctaa gaatggttct gtgtatgtga atgagcgggt ggtaattgtg gctaaatatt
     2101 tttgttttac caagaaactg aaataattct ggccaggaat aaatacttcc tgaacatctt
     2161 aggtcttttc aacaagaaaa agacagagga ttgtccttaa gtccctgcta aaacattcca
     2221 ttgttaaaat ttgcactttg aaggtaagct ttctaggcct gaccctccag gtgtcaatgg
     2281 acttgtgcta ctatattttt ttattcttgg tatcagttta aaattcagac aaggcccaca
     2341 gaataagatt ttccatgcat ttgcaaatac gtatattctt tttccatcca cttgcacaat
     2401 atcattacca tcactttttc atcattcctc agctactact cacattcatt taatggtttc
     2461 tgtaaacatt tttaagacag ttgggatgtc acttaacatt ttttttttga gctaaagtca
     2521 gggaatcaag ccatgcttaa tatttaacaa tcacttatat gtgtgtcgaa gagtttgttt
     2581 tgtttgtcat gtattggtac aagcagatac agtataaact cacaaacaca gatttgaaaa
     2641 taatgcacat atggtgttca aatttgaacc tttctcatgg atttttgtgg tgtgggccaa
     2701 tatggtgttt acattatata attcctgctg tg
//
