LOCUS       pBD21_033               5258 bp    DNA     circular UNA 16-MAR-2018
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...1c-8s9buj3
VERSION     urn.local...1c-8s9buj3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..45
                     /ApEinfo_fwdcolor="#6666ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="Seq3"
     origin          160^161
                     /label="origin"
     CDS             266..1075
                     /invitrogen="""1210000"
                     /modified_by="User"
                     /label="KanR"
     rep_origin      1154..1836
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ColE1 origin"
     misc_feature    2059..2103
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="seq1"
     misc_feature    2198..2241
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="88.64%"
                     /label="tetO reg"
                     /note="Geneious type: regulatory sequence"
     promoter        2198..2271
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="PLtet prom"
                     /Transferred_Similarity="82.43%"
                     /label="PLtet prom"
     reporter        2278..2955
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="DsRed2 reporter"
                     /Transferred_Similarity="74.93%"
                     /modified_by="User"
                     /label="mRFP"
     terminator      3003..3046
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="rrnB_T1 term"
                     /Transferred_Similarity="97.73%"
                     /label="rrnB_T1 term"
     terminator      complement(3003..3046)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="rrnB_T1 term"
                     /Transferred_Similarity="86.36%"
                     /label="rrnB_T1 term"
     terminator      3087..3106
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="stem_loop_early_T7 term"
                     /Transferred_Similarity="100.00%"
                     /label="stem_loop_early_T7 term"
     misc_feature    3238..3282
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Seq2"
     misc_feature    3287..3567
                     /created_by="User"
                     /label="D1 insulator"
     misc_feature    3375..3388
                     /created_by="User"
                     /label="CTCF Sequence"
     promoter        3747..4326
                     /created_by="User"
                     /label="CMV"
     motif           4452..4457
                     /created_by="User"
                     /label="Kozak"
     reporter        4458..5177
                     /created_by="User"
                     /modified_by="User"
                     /label="Sirius"
     misc_feature    5206..5254
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="synth PA"
ORIGIN      
        1 ggtctaatac ccaatctctc gtcttatcca gatgttttat acgccacgaa gtggctcttc
       61 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      121 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc tggcccgtgt ctcaaaatct
      181 ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta
      241 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
      301 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      361 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
      421 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      481 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      541 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
      601 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      661 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      721 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
      781 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
      841 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      901 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
      961 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1021 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1081 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggaagatc
     1141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     1921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     1981 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2041 attcattaat cactctgtgg tttaccgagc tcttattggt tttcaaactt cattgactgt
     2101 gccggagtga gaccgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     2161 taaggatgat ttctggaatt cgcggccgct tctagagtcc ctatcagtga tagagattga
     2221 catccctatc agtgatagag atactgagca ctactagaga aagaggagaa atactagatg
     2281 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
     2341 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
     2401 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
     2461 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
     2521 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
     2581 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
     2641 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
     2701 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
     2761 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
     2821 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
     2881 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
     2941 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
     3001 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     3061 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     3121 tactagtagg atcctaactc gacgtgcagg cttcctcgct cactgactcg ctgcgctcgg
     3181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatggtc tcacgctggt
     3241 gcgtttttat gcttgtagta ttgtataatg tttttaagat ccggagtctt ttgcaatgct
     3301 ctttgggaaa ttatattagc ctaattacta atttcctggc cctcaaggtg acttgcttga
     3361 acttgccacc ttcgccacta gggggcagca ttggtttaca cagggtgaac aaacgttcaa
     3421 ttctaaacta atattctttg gtgggaaagt gtgttcattt tggtttgttt tgaagaagtc
     3481 acttcatacc tttgaactgg ggttaagctg gttaactccc aaaattccac cagttccaat
     3541 atcctatgga aaaaccccaa aaccacttac tggcctaact ggcctcaata ttggccatta
     3601 gccatattat tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     3661 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     3721 tggcattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3781 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3841 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     3901 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     3961 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     4021 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     4081 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     4141 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4201 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     4261 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     4321 cagatcacta gaagctttat tgcggtagtt tatcacagtt aaattgctaa cgcagtcagt
     4381 gggcctcggc ggccaagctt ggcaatccgg tactgttggt aaacagataa gtttgtacaa
     4441 aaaagagagg tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4501 tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg
     4561 agggcgatgc cacctacggc aagctgaccc tgaagctcat ctgcaccacc ggcaagctgc
     4621 ccgtgccctg gcccaccctc gtgaccaccc tgcaattcgg cgtgctgtgc ttcgcccgct
     4681 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4741 aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4801 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4861 gcaacatcct ggggcacaag ctggagtaca acgggataag ctcaaacgta tatatcaccg
     4921 ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg
     4981 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5041 tgctgcccga caaccactac ctgagcgtcc agtccaagct gagcaaagac cccaacgaga
     5101 agcgcgatca catggtcctg ctggagtccg tgaccgccgc cgggatcact ctcggcatgg
     5161 acgagctgta caagtaagct tccactggat tgtacaatta ccaacaataa aatatcttta
     5221 ttttcattac atctgtgtgt tggttttttg tgtgcgct
//
