LOCUS       pUC57-L2               10265 bp    DNA     circular UNA 28-JUN-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...ce-93snh7w
VERSION     urn.local...ce-93snh7w
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          join(<8251..10265,1..>147)
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    142..>147
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    145..147
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    145..8253
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pAGM4673 L2 H4 Copy -
                     Fragment 2 (concatenated sequence 2)"
     misc_feature    complement(155..160)
                     /note="Geneious type: primer"
                     /standard_name="BpiI"
     misc_feature    172..381
                     /note="Geneious type: promoter prokaryotic"
                     /standard_name="Pantoea ananatis promoter"
     enhancer        400..1329
                     /standard_name="crtB"
     RBS             423..431
                     /standard_name="RBS"
     enhancer        1352..2830
                     /standard_name="crtI"
     misc_feature    2853..3998
                     /standard_name="crtY"
     misc_feature    4024..5886
                     /standard_name="dxs K12"
     misc_feature    4404
                     /note="Geneious type: mutation"
                     /standard_name="G to A"
     CDS             5909..6634
                     /standard_name="crtW aa"
     misc_feature    6660..7208
                     /standard_name="idi K12"
     misc_feature    7231..8139
                     /standard_name="crtE"
     terminator      8160..8224
                     /standard_name="kdgm ter"
     misc_feature    8236..8241
                     /note="Geneious type: primer"
                     /standard_name="bpiI"
     misc_feature    8251..8253
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    8251..147
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of >pUC57-Kan - Fragment 2
                     (concatenated sequence 1)"
     misc_feature    <8251..8256
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     rep_origin      complement(8577..9165)
     CDS             complement(9343..10152)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /standard_name="KanR"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg ccactctgtg ccttgtcttc cagtggtatg gggtaccgca
      181 cggtctgcca atccgacgga ggtttatgaa ttttccacct tttccacaag ctcaactagt
      241 attaacgatg tggatttagc aaaaaaaacc tgtaacccta aatgtaaaat aacgggtaag
      301 cctgccaacc atgttatggc agattaagcg tctttttgaa gggcaccgca tctttcgcgt
      361 tgccgtaaat gtatccgttt agcaanvagg adnnnnnnaa tgaataatcc gtcgttactc
      421 aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc ctcaaagtta
      481 tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg ccattgtgac
      541 gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt acaaacgccc
      601 gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg atcgcagatg
      661 cacgaaccgg cgtttgcggc ttttcgggaa gtggctatgg ctcatgatat cgccccggct
      721 tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca atacagccaa
      781 ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt gatgatggcg
      841 caaatcatgg gcgtgcggga taacgccacg ctggaccgcg cctgtgacct tgggctggca
      901 tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg ccgctgttat
      961 ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc ggcacctgaa
     1021 aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga accttactat
     1081 ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc aatcgctacg
     1141 gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca gcaagcctgg
     1201 gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc cgcctctggt
     1261 caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct ctggcagcgc
     1321 ccgctctagg cttactanva ggadnnnnnn aatgaaacca actacggtaa ttggtgcagg
     1381 cttcggtggc ctggcactgg caattcgtct acaagctgcg gggatccccg tcttactgct
     1441 tgaacaacgt gataaacccg gcggtcgggc ttatgtctac gaggatcagg ggtttacctt
     1501 tgatgcaggc ccgacggtta tcaccgatcc cagtgccatt gaagaactgt ttgcactggc
     1561 aggaaaacag ttaaaagagt atgtcgaact gctgccggtt acgccgtttt accgcctgtg
     1621 ttgggagtca gggaaggtct ttaattacga taacgatcaa acccggctcg aagcgcagat
     1681 tcagcagttt aatccccgcg atgtcgaagg ttatcgtcag tttctggact attcacgcgc
     1741 ggtgtttaaa gaaggctatc taaagctcgg tactgtccct tttttatcgt tcagagacat
     1801 gcttcgcgcc gcacctcaac tggcgaaact gcaggcatgg agaagcgttt acagtaaggt
     1861 tgccagttac atcgaagatg aacatctgcg ccaggcgttt tctttccact cgctgttggt
     1921 gggcggcaat cccttcgcca cctcatccat ttatacgttg atacacgcgc tggagcgtga
     1981 gtggggcgtc tggtttccgc gtggcggcac cggcgcatta gttcagggga tgataaagct
     2041 gtttcaggat ctgggtggcg aagtcgtgtt aaacgccaga gtcagccata tggaaacgac
     2101 aggaaacaag attgaagccg tgcatttaga ggacggtcgc aggttcctga cgcaagccgt
     2161 cgcgtcaaat gcagatgtgg ttcataccta tcgcgacctg ttaagccagc accctgccgc
     2221 ggttaagcag tccaacaaac tgcagactaa gcgcatgagt aactctctgt ttgtgctcta
     2281 ttttggtttg aatcaccatc atgatcagct cgcgcatcac acggtttgtt tcggcccgcg
     2341 ttaccgcgag ctgattgacg aaatttttaa tcatgatggc ctcgcagagg acttctcact
     2401 ttatctgcac gcgccctgtg tcacggattc gtcactggcg cctgaaggtt gcggcagtta
     2461 ctatgtgttg gcgccggtgc cgcatttagg caccgcgaac ctcgactgga cggttgaggg
     2521 gccaaaacta cgcgaccgta tttttgcgta ccttgagcag cattacatgc ctggcttacg
     2581 gagtcagctg gtcacgcacc ggatgtttac gccgtttgat tttcgcgacc agcttaatgc
     2641 ctatcatggc tcagcctttt ctgtggagcc cgttcttacc cagagcgcct ggtttcggcc
     2701 gcataaccgc gataaaacca ttactaatct ctacctggtc ggcgcaggca cgcatcccgg
     2761 cgcaggcatt cctggcgtca tcggctcggc aaaagcgaca gcaggtttga tgctggagga
     2821 tctgatatga gcttttacnv aggadnnnnn naatgcaacc gcattatgat ctgattctcg
     2881 tgggggctgg actcgcgaat ggccttatcg ccctgcgact tcagcagcag caacctgata
     2941 tgcgtatttt gcttatcgac gccgcacccc aggcgggcgg gaatcatacg tggtcatttc
     3001 accacgatga tttgactgag agccaacatc gttggatagc tccgctggtg gttcatcact
     3061 ggcccgacta tcaggtacgc tttcccacac gccgtcgtaa gctgaacagc ggctactttt
     3121 gtattacttc tcagcgtttc gctgaggttt tacagcgaca gtttggcccg cacttgtgga
     3181 tggataccgc ggtcgcagag gttaatgcgg aatctgttcg gttgaaaaag ggtcaggtta
     3241 tcggtgcccg cgcggtgatt gacgggcggg gttatgcggc aaattcagca ctgagcgtgg
     3301 gcttccaggc gtttattggc caggaatggc gattgagtca cccgcatggt ttatcatctc
     3361 cgattatcat ggatgccacg gtcgatcagc aaaatggtta tcgcttcgtg tacagcctgc
     3421 cgctctcgcc gaccagattg ttaattgaag atacgcacta tattgataat gcgacattag
     3481 atcctgaatg cgcgcggcaa aatatttgcg actatgccgc gcaacagggt tggcagcttc
     3541 agacactgct gcgagaagaa cagggcgcct tacccattac tctgtcgggc aatgccgacg
     3601 cattctggca gcagcgcccc ctggcctgta gtggattacg tgccggtctg ttccatccta
     3661 ccaccggcta ttcactgccg ctggcggttg ccgtggccga ccgcctgagt gcacttgatg
     3721 tctttacgtc ggcctcaatt caccatgcca ttacgcattt tgcccgcgag cgctggcagc
     3781 agcagggctt tttccgcatg ctgaatcgca tgctgttttt agccggaccc gccgattcac
     3841 gctggcgggt tatgcagcgt ttttatggtt tacctgaaga tttaattgcc cgtttttatg
     3901 cgggaaaact cacgctgacc gatcggctac gtattctgag cggcaagccg cctgttccgg
     3961 tattagcagc attgcaagcc attatgacga ctcatcgtta agcttcagan vaggadnnnn
     4021 nnaatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc cacccaggag
     4081 ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg ccgctattta
     4141 ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac ggtcgaactg
     4201 accgtggcgc tgcactatgt ctacaacacc ccgtttgacc aattgatttg ggatgtgggg
     4261 catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg caccatccgt
     4321 cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga cgtattaagc
     4381 gtcgggcatt catcaacctc cattagtgcc ggaattggta ttgcggttgc tgccgaaaaa
     4441 gaaggcaaaa atcgccgcac cgtctgtgtc attggcgatg gcgcgattac cgcaggcatg
     4501 gcgtttgaag cgatgaatca cgcgggcgat atccgtcctg atatgctggt gattctcaac
     4561 gacaatgaaa tgtcgatttc cgaaaatgtc ggcgcgctca acaaccatct ggcacagctg
     4621 ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt ctctggcgtg
     4681 ccgccaatta aagagctgct caaacgcacc gaagaacata ttaaaggcat ggtagtgcct
     4741 ggcacgttgt ttgaggagct gggctttaac tacatcggcc cggtggacgg tcatgatgtg
     4801 ctggggctta tcaccacgct aaagaacatg cgcgacctga aaggcccgca gttcctgcat
     4861 atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccgat cactttccat
     4921 gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg cggtttgccg
     4981 agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga caacaagctg
     5041 atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc acgtaaattc
     5101 ccggatcgct acttcgacgt ggcaattgcc gagcaacatg cggtgacctt tgctgcgggt
     5161 ctggcgattg gtgggtacaa acccattgtc gcgatttact ccactttcct gcaacgcgcc
     5221 tatgatcagg tgctgcatga cgtggcgatt caaaagcttc cggtcctgtt cgccatcgac
     5281 cgcgcgggca ttgttggtgc tgacggtcaa acccatcagg gtgcttttga tctctcttac
     5341 ctgcgctgca taccggaaat ggtcattatg accccgagcg atgaaaacga atgtcgccag
     5401 atgctctata ccggctatca ctataacgat ggcccgtcag cggtgcgcta cccgcgtggc
     5461 aacgcggtcg gcgtggaact gacgccgctg gaaaaactac caattggcaa aggcattgtg
     5521 aagcgtcgtg gcgagaaact ggcgatcctt aactttggta cgctgatgcc agaagcggcg
     5581 aaagtcgccg aatcgctgaa cgccacgctg gtcgatatgc gttttgtgaa accgcttgat
     5641 gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt agaagaaaac
     5701 gccattatgg gcggcgcagg cagcggcgtg aacgaagtgc tgatggccca tcgtaaacca
     5761 gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac tcaggaagaa
     5821 atgcgcgccg aactcggcct cgatgccgct ggtatggaag ccaaaatcaa ggcctggctg
     5881 gcataagctt tgtgnvagga dnnnnnnaat gagcgcacat gccctgccca aggcagatct
     5941 gaccgccacc agcctgatcg tatcgggcgg catcatcgcc gcttggctgg ccctgcatgt
     6001 gcatgcgctg tggtttctgg acgcagcggc gcatcccatc ctggcgatcg caaatttcct
     6061 ggggctgacc tggctgtcgg tcggattgtt catcatcgcg catgacgcga tgcacgggtc
     6121 ggtggtgccg gggcgtccgc gcgccaatgc ggcgatgggc cagcttgtcc tgtggctgta
     6181 tgccggattt tcgtggcgca agatgatcgt caagcacatg gcccatcacc gccatgccgg
     6241 aaccgacgac gaccccgatt tcgaccatgg cggcccggtc cgctggtacg cccgcttcat
     6301 cggcacctat ttcggctggc gcgaggggct gcttctgccc gtcatcgtga cggtctatgc
     6361 gctgatcctt ggggatcgct ggatgtacgt ggtattctgg ccgctgccgt cgatcctggc
     6421 gtcgatccag ctgttcgtgt tcggcacctg gctgccgcac cgccccggcc acgacgcgtt
     6481 cccggaccgc cacaatgcgc ggtcgtcgcg gatcagcgac cccgtgtcgc tgctgacctg
     6541 ttttcacttt ggcggttatc atcacgaaca ccacctgcac ccgacggtgc cgtggtggcg
     6601 cctgcccagc acccgcacca agggcgacac cgcataagct tgagcnvagg adnnnnnnaa
     6661 tgcaaacgga acacgtcatt ttattgaatg cacagggagt tcccacgggt acgctggaaa
     6721 agtatgccgc acacacggca gacacccgct tacatctcgc gttctccagt tggctgttta
     6781 atgccaaagg acaattatta gttacccgcc gcgcactgag caaaaaagca tggcctggcg
     6841 tgtggactaa ctcggtttgt gggcacccac aactgggaga aagcaacgag gacgcagtga
     6901 tccgccgttg ccgttatgag cttggcgtgg aaattacgcc tcctgaatct atctatcctg
     6961 actttcgcta ccgcgccacc gatccgagtg gcattgtgga aaatgaagtg tgtccggtat
     7021 ttgccgcacg caccactagt gcgttacaga tcaatgatga tgaagtgatg gattatcaat
     7081 ggtgtgattt agcagatgta ttacacggta ttgatgcaac gccgtgggcg ttcagtccgt
     7141 ggatggtgat gcaggcgaca aatcgcgaag ccagaaaacg attatctgca tttacccagc
     7201 ttaaataagc tttgccnvag gadnnnnnna atgacggtct gcgcaaaaaa acacgttcat
     7261 ctcactcgcg atgctgcgga gcagttactg gctgatattg atcgacgcct tgatcagtta
     7321 ttgcccgtgg agggagaacg ggatgttgtg ggtgccgcga tgcgtgaagg tgcgctggca
     7381 ccgggaaaac gtattcgccc catgttgctg ttgctgaccg cccgcgatct gggttgcgct
     7441 gtcagccatg acggattact ggatttggcc tgtgcggtgg aaatggtcca cgcggcttcg
     7501 ctgatccttg acgatatgcc ctgcatggac gatgcgaagc tgcggcgcgg acgccctacc
     7561 attcattctc attacggaga gcatgtggca atactggcgg cggttgcctt gctgagtaaa
     7621 gcctttggcg taattgccga tgcagatggc ctcacgccgc tggcaaaaaa tcgggcggtt
     7681 tctgaactgt caaacgccat cggcatgcaa ggattggttc agggtcagtt caaggatctg
     7741 tctgaagggg ataagccgcg cagcgctgaa gctattttga tgacgaatca ctttaaaacc
     7801 agcacgctgt tttgtgcctc gatgcagatg gcctcgattg ttgcgaatgc ctccagcgaa
     7861 gcgcgtgatt gcctgcatcg tttttcactt gatcttggtc aggcatttca actgctggac
     7921 gatttgaccg atggcatgac cgacaccggt aaggatagca atcaggacgc cggtaaatcg
     7981 acgctggtca atctgttagg cccgagggcg gttgaagaac gtctgagaca acatcttcag
     8041 cttgccagtg agcatctctc tgcggcctgc caacacgggc acgccactca acattttatt
     8101 caggcctggt ttgacaaaaa actcgctgcc gtcagttaag cttgcaagaa ttcaagcttg
     8161 gagtacgtta attagttgtc tgttttaagc cccgtgctct ccgccggggc ttttttatta
     8221 aaaaccactc aagtggaaga caagggacac gaagtgtggg gtgcctaatg agtgagctaa
     8281 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8341 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8401 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8461 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8581 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8641 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8701 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8761 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8821 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8881 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8941 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9001 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9061 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9301 agcccaatct gaataatgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
     9361 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     9421 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     9481 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     9541 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     9601 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     9661 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     9721 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     9781 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     9841 tgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     9901 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     9961 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
    10021 cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
    10081 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg tttcccgttg
    10141 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
    10201 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag
    10261 ctgca
//
