LOCUS pUC57-L1P3 2936 bp DNA circular UNA 25-JUL-2018 DEFINITION Concatenation of 2 sequences. ACCESSION urn.local...dl-9ax8ipr VERSION urn.local...dl-9ax8ipr KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 2934..646 /note="Geneious type: Concatenated sequence" /standard_name="Digestion of pICH47742 - L1P2 (reversed) - Fragment 2 (concatenated sequence 1)" misc_feature 59..595 /standard_name="lacZ" misc_feature 644..2936 /note="Geneious type: Concatenated sequence" /standard_name="Digestion of pUC57 amp amplif with primers - Fragment 2 (concatenated sequence 2)" misc_feature 644..646 /note="Geneious type: ligation" /standard_name="Ligation" source <680..2789 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" rep_origin complement(970..1558) CDS complement(1729..2589) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 1650 .. 2441 / #ccffcc 2: 2442 .. 2510 / #ccffcc / signal sequence Cleavage site after base 2441" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /standard_name="AmpR" promoter complement(2590..2694) /gene="bla" /note="color: #ffffff; direction: LEFT" /standard_name="AmpR promoter" source 2790..>2900 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 2934..2936 /note="Geneious type: ligation" /standard_name="Ligation" ORIGIN 1 gtgaagacaa actagaattc gagctcggag tgagaccgca gctggcacga caggtttccc 61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 181 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc 241 gactctagag gatccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg 301 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 361 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 421 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 541 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 601 cagacaagct gtgacggtct cacgctttac ttgtcttctg cacgaagtgt ggggtgccta 661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 781 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 1021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 1081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 1141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 1201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 1261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 1321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 1381 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 1441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 1501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 1561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 1621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 1681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 1741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 1801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 1861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 1921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 1981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 2041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 2101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 2161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 2221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 2281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 2341 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 2401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 2461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 2521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 2581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 2641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 2701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 2761 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 2821 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 2881 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc cactct //