LOCUS       pUC57-L1P5              2936 bp    DNA     circular UNA 25-JUL-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...dn-9ax8ipr
VERSION     urn.local...dn-9ax8ipr
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    2934..646
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pICH47742 - L1P2 (reversed) -
                     Fragment 2 (concatenated sequence 1)"
     misc_feature    59..595
                     /standard_name="lacZ"
     misc_feature    644..2936
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pUC57 amp amplif with primers
                     - Fragment 2 (concatenated sequence 2)"
     misc_feature    644..646
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     source          <680..2789
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     rep_origin      complement(970..1558)
     CDS             complement(1729..2589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="This feature has 2 segments: 1: 1650 .. 2441 /
                     #ccffcc 2: 2442 .. 2510 / #ccffcc / signal sequence
                     Cleavage site after base 2441"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     promoter        complement(2590..2694)
                     /gene="bla"
                     /note="color: #ffffff; direction: LEFT"
                     /standard_name="AmpR promoter"
     source          2790..>2900
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    2934..2936
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gtgaagacaa cagagaattc gcatgcggag tgagaccgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc
      241 gactctagag gatccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg
      301 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      361 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      421 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      541 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      601 cagacaagct gtgacggtct cacgcttgtg ttgtcttctg cacgaagtgt ggggtgccta
      661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      781 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1381 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2341 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2761 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     2821 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     2881 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc cactct
//
