LOCUS       Exported               13318 bp ds-DNA     circular SYN 09-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pT2K-CAGGS-LRL-Mouse Myc-IRES-Luciferase 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2018 from SnapGene Viewer 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5557..6415
                     /organism="Mammalian expression vector pCAGGS"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2453."
                     /db_xref="taxon:1944753"
     source          6459..7823
                     /organism="Mus musculus"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="NIH_MGC_378"
                     /clone="MGC:170559 IMAGE:8861954"
                     /tissue_type="Brain, PCR rescued clones"
                     /note="Vector: pCR4-TOPO with reversed insert; Clone 
                     identification sequence tag: TTTGGCTG"
                     /db_xref="taxon:10090"
     source          8407..10058
                     /organism="Firefly luciferase reporter vector 
                     pGL4.51[luc2/CMV/Neo]"
                     /mol_type="other DNA"
                     /db_xref="taxon:554673"
     rep_origin      complement(7..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        3081..3460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3463..3738
                     /label=chicken beta-actin promoter
     promoter        3750..4786
                     /label=beta actin promoter
     misc_feature    4800..4805
                     /label=EcoRI(4800)
     misc_feature    4806..4811
                     /label=XhoI(4806)
     misc_feature    4824..4857
                     /label=LoxP
     misc_feature    4858..4863
                     /label=XhoI(4858)
     CDS             4874..5551
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     regulatory      5557..5648
                     /regulatory_class="terminator"
                     /note="3' UTR of rabbit beta-globin, incl. polyA"
     misc_feature    5649..6005
                     /label=3' flanking region of rabbit beta-globin
                     /note="3' flanking region of rabbit beta-globin"
     rep_origin      6307..6382
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      6413..6415
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     misc_feature    complement(6416..6449)
                     /label=LoxP
     misc_feature    6450..6455
                     /label=EcoRI(6450)
     CDS             6459..7823
                     /codon_start=1
                     /gene="Myc"
                     /gene_synonym="c-myc;Myc2"
                     /product="myelocytomatosis oncogene"
                     /label=mouse Myc
                     /note="alternative start codon"
                     /db_xref="GeneID:17869"
                     /db_xref="MGI:MGI:97250"
                     /protein_id="AAI38933.1"
                     /translation="MDFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEEN
                     FYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDG
                     GGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSE
                     KLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPK
                     SCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVS
                     VEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRA
                     KLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPEL
                     ENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
     misc_feature    7824..7829
                     /label=XhoI(7824)
     misc_feature    7844..8406
                     /label=IRES
     CDS             8407..10058
                     /codon_start=1
                     /transl_table=11
                     /gene="luc2"
                     /product="firefly luciferase"
                     /label=Luciferase 2
                     /note="luminescent reporter protein; derived from Photinus 
                     pyralis"
                     /protein_id="ACH53166.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            8407..10058
                     /gene="luc2"
                     /label=luc2
     misc_feature    8998..9003
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9139..9141
                     /gene="luc2"
                     /note="AMP binding site [chemical binding]; other site"
                     /db_xref="CDD:213279"
     misc_feature    9262..9264
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9346..9360
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9418..9435
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9442..9444
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9664..9666
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9670..9672
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9706..9708
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    9715..9717
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     primer_bind     complement(10060..10062)
                     /label=SJVL-749_Max=
                     /note="Venus YFP pcr right. Max recorded sequencing read = 
                     bases. Perfect match Tm = 79.9 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCGGCCGCTC
                     ACTTGTACAG CTCGTCCATG CCG. This primer does not match this 
                     site completely."
     polyA_site      10125..10180
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(11081..11097)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11134..11152)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11173..11189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11197..11213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11221..11251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11266..11287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11575..12163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12334..13194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13195..13299)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgtcgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggttgg ttggtagatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattgattga ttgattgatt gattgattga ttgattgatt ggtagtcaaa ataagaaata
     1561 atttccacag attcattaca gaaatgatta aatgcataca taaaaaactg gggggggggg
     1621 gatacaacaa cacacttaag tcacatttgc ctacgtaaag aaaagtaaag aaaatcaata
     1681 gctatatttt acatctcctt tttttgctgt cttttaatta gccttgtttt gctgttatct
     1741 tgattgaaac tgtaacttct tcacctgctt cttttctttg taggttttgc cagcccagaa
     1801 acaggcatgg ctgtgcaagg ccagagcacc atgcacaatg cgctgcatgt cttcatgaac
     1861 ggctcaatgt cctcagtcca gggctcagcc aacgacccca tcttcctcct tcaccatgct
     1921 ttcattgaca ggtaacaaac acgtcatgac attagactgc acagtttttg acaaagttca
     1981 tacaatctgt tgtttatagc tgctacaatt agtgaagttt gtgaatgtac ttggatgagc
     2041 agcgaaagat caattgagat caattgttag agtttggttg ccctgcagag caaagaacaa
     2101 aaaataatct ggtggcttta ctgcgtgagg ttattattgg tggaatagaa acacaaaaca
     2161 taattgcatt tatttgttta attttttatc ttatcttaac tttcatcttg catatttgtt
     2221 tcttacatca tttctagcat ctttgagcgc tggctaagaa ctcatcagcc tccccggtcc
     2281 atctacccac gtaccaatgc accaattggc cacaatgacg gctactacat ggtgccattc
     2341 cttcctcttt ataggaatgg agactacctc ctgtccaaca aggctcttgg atacgagtac
     2401 gcctacctgt tggacccagg tcattgcaca acaccagaaa tgccctctga tctgcaaaag
     2461 acgtgaatat ctgttcagac acccatatcc actctgttcc acacaggtca gaggtttgtc
     2521 caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa
     2581 gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga
     2641 gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa
     2701 agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga
     2761 aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag
     2821 acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca
     2881 gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca
     2941 atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct
     3001 tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac
     3061 aggccagatg ggccctcgtc gacattgatt attgactagt tattaatagt aatcaattac
     3121 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3181 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3241 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac
     3301 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3361 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3421 ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt
     3481 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     3541 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg
     3601 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
     3661 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     3721 cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc
     3781 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     3841 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt
     3901 ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg
     3961 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc
     4021 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg
     4081 ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg
     4141 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta
     4201 acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     4261 tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     4321 tgccgcgcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     4381 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     4441 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg
     4501 cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa
     4561 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc
     4621 ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     4681 ctggcgtgtg accggcggct ctagctagag cctctgctaa ccatgttcat gccttcttct
     4741 ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     4801 aattcctcga gtctagatcg cgtataactt cgtataatgt atgctatacg aagttatctc
     4861 gagtctagga tccatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt
     4921 gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4981 cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt
     5041 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
     5101 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5161 gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg
     5221 ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat
     5281 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     5341 gctgaagggc gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga
     5401 gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
     5461 cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg
     5521 caccgagggc cgccaccacc tgttcctgta ggaattgatc tttttccctc tgccaaaaat
     5581 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5641 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5701 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     5761 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     5821 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     5881 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     5941 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     6001 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6121 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6181 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     6241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacataac
     6421 ttcgtataat gtatgctata cgaagttatg aattccggct ggatttcctt tgggcgttgg
     6481 aaaccccgca gacagccacg acgatgcccc tcaacgtgaa cttcaccaac aggaactatg
     6541 acctcgacta cgactccgta cagccctatt tcatctgcga cgaggaagag aatttctatc
     6601 accagcaaca gcagagcgag ctgcagccgc ccgcgcccag tgaggatatc tggaagaaat
     6661 tcgagctgct tcccaccccg cccctgtccc cgagccgccg ctccgggctc tgctctccat
     6721 cctatgttgc ggtcgctacg tccttctccc caagggaaga cgatgacggc ggcggtggca
     6781 acttctccac cgccgatcag ctggagatga tgaccgagtt acttggagga gacatggtga
     6841 accagagctt catctgcgat cctgacgacg agaccttcat caagaacatc atcatccagg
     6901 actgtatgtg gagcggtttc tcagccgctg ccaagctggt ctcggagaag ctggcctcct
     6961 accaggctgc gcgcaaagac agcaccagcc tgagccccgc ccgcgggcac agcgtctgct
     7021 ccacctccag cctgtacctg caggacctca ccgccgccgc gtccgagtgc attgacccct
     7081 cagtggtctt tccctacccg ctcaacgaca gcagctcgcc caaatcctgt acctcgtccg
     7141 attccacggc cttctctcct tcctcggact cgctgctgtc ctccgagtcc tccccacggg
     7201 ccagccctga gcccctagtg ctgcatgagg agacaccgcc caccaccagc agcgactctg
     7261 aagaagagca agaagatgag gaagaaattg atgtggtgtc tgtggagaag aggcaaaccc
     7321 ctgccaagag gtcggagtcg ggctcatctc catcccgagg ccacagcaaa cctccgcaca
     7381 gcccactggt cctcaagagg tgccacgtct ccactcacca gcacaactac gccgcacccc
     7441 cctccacaag gaaggactat ccagctgcca agagggccaa gttggacagt ggcagggtcc
     7501 tgaagcagat cagcaacaac cgcaagtgct ccagccccag gtcctcagac acggaggaaa
     7561 acgacaagag gcggacacac aacgtcttgg aacgtcagag gaggaacgag ctgaagcgca
     7621 gcttttttgc cctgcgtgac cagatccctg aattggaaaa caacgaaaag gcccccaagg
     7681 tagtgatcct caaaaaagcc accgcctaca tcctgtccat tcaagcagac gagcacaagc
     7741 tcacctctga aaaggactta ttgaggaaac gacgagaaca gttgaaacac aaactcgaac
     7801 agcttcgaaa ctctggtgca taactcgagc gggatcaatt ccgccccccc cctaacgtta
     7861 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     7921 tattgccgtc ttttgncaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     7981 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     8041 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     8101 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     8161 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     8221 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     8281 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     8341 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     8401 aataccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     8461 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
     8521 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     8581 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     8641 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     8701 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     8761 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     8821 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     8881 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     8941 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     9001 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     9061 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     9121 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     9181 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     9241 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     9301 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     9361 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     9421 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     9481 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     9541 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     9601 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     9661 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     9721 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     9781 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     9841 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     9901 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     9961 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
    10021 ctcattaagg ccaagaaggg cggcaagatc gccgtgtagt aattttccct ctgccaaaaa
    10081 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
    10141 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
    10201 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat
    10261 atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc
    10321 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
    10381 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
    10441 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
    10501 gatccctcga cctgcagccc aagctgatct gcgaagatac ggccacgggt gctcttgatc
    10561 ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat
    10621 tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt
    10681 gtggtaatag atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt
    10741 tcatataatg tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc
    10801 atcagcgcaa ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa
    10861 aaaataagta ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct
    10921 aaaaaaatgt aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta
    10981 aaaatcccca aaaataatac ttaagtacag taatcaagta aaattactca agtactttac
    11041 acctctggtt cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg
    11101 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
    11161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtg
//
