LOCUS       Exported               11960 bp ds-DNA     circular SYN 09-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pT2K-CAGGS-LRL-IRES-Luciferase 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2018 from SnapGene Viewer 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5557..6415
                     /organism="Mammalian expression vector pCAGGS"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2453."
                     /db_xref="taxon:1944753"
     source          7049..8700
                     /organism="Firefly luciferase reporter vector 
                     pGL4.51[luc2/CMV/Neo]"
                     /mol_type="other DNA"
                     /db_xref="taxon:554673"
     rep_origin      complement(7..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        3081..3460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3463..3738
                     /label=chicken beta-actin promoter
     misc_feature    3669..3687
                     /label=CAG-Pro5' rev
     misc_feature    3669..3687
                     /label=CAG-Pro5' fw
     promoter        3750..4786
                     /label=beta actin promoter
     misc_feature    4218..4233
                     /label=CAG-Prom 1 Forw
     misc_feature    4417..4434
                     /label=CAG-Proirv1
     misc_feature    4671..4693
                     /label=pCAGGS-5 forw
     misc_feature    4728..4746
                     /label=pCAG forw (sequiserve)
     misc_feature    4728..4746
                     /label=CAG Forw
     misc_feature    4801..4805
                     /label=EcoRI(4801)
     misc_feature    4806..4811
                     /label=XhoI(4806)
     misc_feature    4824..4857
                     /label=LoxP
     misc_feature    4858..4863
                     /label=XhoI(4858)
     CDS             4874..5551
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     misc_feature    5077..5098
                     /label=DsRed2 Forw(no Rev)
     misc_feature    complement(5077..5097)
                     /label=DsRed2 Reverse
     misc_feature    5287..5305
                     /label=dsRed2 Forw 1
     misc_feature    5351..5371
                     /label=dsRed2 Forw2
     misc_feature    5366..5386
                     /label=DsRed2 Forw
     regulatory      5557..5648
                     /regulatory_class="terminator"
                     /note="3' UTR of rabbit beta-globin, incl. polyA"
     misc_feature    5649..6005
                     /label=3' flanking region of rabbit beta-globin
                     /note="3' flanking region of rabbit beta-globin"
     rep_origin      6307..6382
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      6413..6415
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     misc_feature    complement(6416..6449)
                     /label=LoxP
     misc_feature    6456..6461
                     /label=EcoRI(6456)
     misc_feature    6466..6471
                     /label=XhoI(6466)
     misc_feature    6486..7048
                     /label=IRES
     misc_feature    6643..6662
                     /label=IRES Forw2 (no Rev2)
     misc_feature    complement(6643..6662)
                     /label=IRES Reverse2
     CDS             7049..8700
                     /codon_start=1
                     /transl_table=11
                     /gene="luc2"
                     /product="firefly luciferase"
                     /label=luciferase 2
                     /note="luminescent reporter protein; derived from Photinus 
                     pyralis"
                     /protein_id="ACH53166.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            7049..8700
                     /gene="luc2"
                     /label=luc2
     misc_feature    7640..7645
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    7904..7906
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    7988..8002
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    8060..8077
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    8084..8086
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    8306..8308
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    8312..8314
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    8348..8350
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     misc_feature    8357..8359
                     /gene="luc2"
                     /label=active site
                     /note="active site"
                     /db_xref="CDD:213279"
     primer_bind     complement(8702..8704)
                     /label=SJVL-749_Max=
                     /note="Venus YFP pcr right. Max recorded sequencing read = 
                     bases. Perfect match Tm = 79.9 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCGGCCGCTC
                     ACTTGTACAG CTCGTCCATG CCG. This primer does not match this 
                     site completely."
     polyA_site      8767..8822
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    complement(8821..8840)
                     /label=rbglobpA Reverse
     primer_bind     complement(9723..9739)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9776..9794)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9815..9831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9839..9855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9863..9893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9908..9929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10217..10805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10976..11836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11837..11941)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgtcgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggttgg ttggtagatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattgattga ttgattgatt gattgattga ttgattgatt ggtagtcaaa ataagaaata
     1561 atttccacag attcattaca gaaatgatta aatgcataca taaaaaactg gggggggggg
     1621 gatacaacaa cacacttaag tcacatttgc ctacgtaaag aaaagtaaag aaaatcaata
     1681 gctatatttt acatctcctt tttttgctgt cttttaatta gccttgtttt gctgttatct
     1741 tgattgaaac tgtaacttct tcacctgctt cttttctttg taggttttgc cagcccagaa
     1801 acaggcatgg ctgtgcaagg ccagagcacc atgcacaatg cgctgcatgt cttcatgaac
     1861 ggctcaatgt cctcagtcca gggctcagcc aacgacccca tcttcctcct tcaccatgct
     1921 ttcattgaca ggtaacaaac acgtcatgac attagactgc acagtttttg acaaagttca
     1981 tacaatctgt tgtttatagc tgctacaatt agtgaagttt gtgaatgtac ttggatgagc
     2041 agcgaaagat caattgagat caattgttag agtttggttg ccctgcagag caaagaacaa
     2101 aaaataatct ggtggcttta ctgcgtgagg ttattattgg tggaatagaa acacaaaaca
     2161 taattgcatt tatttgttta attttttatc ttatcttaac tttcatcttg catatttgtt
     2221 tcttacatca tttctagcat ctttgagcgc tggctaagaa ctcatcagcc tccccggtcc
     2281 atctacccac gtaccaatgc accaattggc cacaatgacg gctactacat ggtgccattc
     2341 cttcctcttt ataggaatgg agactacctc ctgtccaaca aggctcttgg atacgagtac
     2401 gcctacctgt tggacccagg tcattgcaca acaccagaaa tgccctctga tctgcaaaag
     2461 acgtgaatat ctgttcagac acccatatcc actctgttcc acacaggtca gaggtttgtc
     2521 caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa
     2581 gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga
     2641 gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa
     2701 agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga
     2761 aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag
     2821 acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca
     2881 gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca
     2941 atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct
     3001 tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac
     3061 aggccagatg ggccctcgtc gacattgatt attgactagt tattaatagt aatcaattac
     3121 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3181 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3241 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac
     3301 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3361 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3421 ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt
     3481 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     3541 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg
     3601 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
     3661 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     3721 cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc
     3781 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     3841 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt
     3901 ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg
     3961 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc
     4021 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg
     4081 ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg
     4141 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta
     4201 acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     4261 tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     4321 tgccgcgcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     4381 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     4441 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg
     4501 cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa
     4561 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc
     4621 ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     4681 ctggcgtgtg accggcggct ctagctagag cctctgctaa ccatgttcat gccttcttct
     4741 ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     4801 aattcctcga gtctagatcg cgtataactt cgtataatgt atgctatacg aagttatctc
     4861 gagtctagga tccatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt
     4921 gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4981 cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt
     5041 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
     5101 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5161 gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg
     5221 ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat
     5281 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     5341 gctgaagggc gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga
     5401 gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
     5461 cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg
     5521 caccgagggc cgccaccacc tgttcctgta ggaattgatc tttttccctc tgccaaaaat
     5581 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5641 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5701 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     5761 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     5821 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     5881 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     5941 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     6001 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6121 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6181 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     6241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacataac
     6421 ttcgtataat gtatgctata cgaagttatg agctcgaatt cagatctcga gcgggatcaa
     6481 ttccgccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6541 gtctatatgt tattttccac catattgccg tcttttgnca atgtgagggc ccggaaacct
     6601 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6661 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6721 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6781 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6841 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6901 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6961 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     7021 gttttccttt gaaaaacacg ataataccat ggaagatgcc aaaaacatta agaagggccc
     7081 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
     7141 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
     7201 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
     7261 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
     7321 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
     7381 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
     7441 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
     7501 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
     7561 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
     7621 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
     7681 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
     7741 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
     7801 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
     7861 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
     7921 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
     7981 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
     8041 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
     8101 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
     8161 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg
     8221 cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct
     8281 catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca
     8341 cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc
     8401 agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg
     8461 cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa
     8521 aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa
     8581 gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga
     8641 cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta
     8701 gtaattttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     8761 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     8821 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     8881 agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt
     8941 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     9001 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     9061 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     9121 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagctgat ctgcgaagat
     9181 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     9241 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     9301 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     9361 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     9421 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     9481 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     9541 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     9601 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     9661 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     9721 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     9781 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9961 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10621 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    10681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11281 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11581 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11941 gcacatttcc ccgaaaagtg
//
