LOCUS       pRRL-TetO.Pax7.I        9556 bp    DNA     circular     22-MAR-2018
SOURCE
  ORGANISM
KEYWORDS
COMMENT     
FEATURES             Location/Qualifiers
     misc_feature    4060..4638
                     /label="Ires"
     misc_feature    5408..5983
                     /label="wpre"
     misc_feature    2466..4022
                     /label="Pax7"
     misc_feature    4647..5366
                     /label="eGFP"
     misc_feature    2006..2311
                     /label="Tre"
     misc_feature    2312..2431
                     /label="cmv2"
     misc_feature    6085..6318
                     /label="LTR"
     misc_feature    6530..6607
                     /label="SV40 ori"
     misc_feature    1842..1857
                     /label="cppt"
     misc_feature    6068..6083
                     /label="cppt"
     misc_feature    236..416
                     /label="LTR"
     misc_feature    7476..8336
                     /label="Amp-R"
     misc_feature    8491..9110
                     /label="pBR322 ori"
     misc_binding    5400..5405
                     /label="SalI"
     misc_binding    922..929
                     /label="NotI"
     misc_binding    2297..2302
                     /label="KpnI"
     misc_binding    2486..2491
                     /label="KpnI"
     misc_binding    4502..4507
                     /label="KpnI"
     misc_binding    6014..6019
                     /label="KpnI"
     misc_binding    5356..5361
                     /label="BsrGI"
     misc_binding    2454..2459
                     /label="BamHI"
     misc_binding    4023..4028
                     /label="XhoI"
     misc_feature    4106..4123
                     /label="Ires-rev"
     misc_feature    4530..4551
                     /label="Ires-for"
     misc_feature    3836..3854
                     /label="Pax-for."
     misc_feature    4196..4213
                     /label="Rev.1"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg 
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag 
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc 
     1801 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg 
     1861 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag 
     1921 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag 
     1981 atccagtttg gttatcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 
     2041 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca 
     2101 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa 
     2161 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 
     2221 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa 
     2281 agtgaaagtc gagctcggta cccgggtcga gtaggcgtgt acggtgggag gcctatataa 
     2341 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2401 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgtt aacggatccg 
     2461 ccaccatggc ggcccttccc ggcacggtac cgagaatgat gcggccggct ccggggcaga 
     2521 actacccccg cacgggattc cctttggaag tgtccacccc gcttggccaa ggccgggtca 
     2581 atcagctggg aggggtcttc atcaatgggc gacccctgcc taaccacatc cgccacaaga 
     2641 tagtggagat ggcccaccat ggcatccggc cctgtgtcat ctcccgacag ctgcgtgtct 
     2701 cccacggctg cgtctccaag attctttgcc gctaccagga gaccgggtcc atccggcctg 
     2761 gggccatcgg cggcagcaag cccagacagg tggcgactcc ggatgtagag aaaaagattg 
     2821 aggagtacaa gagggaaaac ccaggcatgt tcagctggga gatccgggac aggctgctga 
     2881 aggatgggca ctgtgaccga agcactgtgc cctcagtgag ttcgattagc cgcgtgctca 
     2941 gaatcaagtt cgggaagaaa gaggaggagg atgaagcgga caagaaggag gacgacggcg 
     3001 aaaagaaggc caaacacagc atcgacggca tcctgggcga caaagggaac cggctggacg 
     3061 agggctcgga tgtggagtcg gaacctgacc tcccactgaa gcgcaagcag cgacgcagtc 
     3121 ggaccacatt cacggccgag cagctggagg agctggagaa ggcctttgag aggacccact 
     3181 acccagacat atacacccgc gaggagctgg cgcagaggac caagctgaca gaggcgcgtg 
     3241 tgcaggtctg gttcagtaac cgccgcgccc gttggcgtaa gcaggcagga gccaaccagc 
     3301 tggcggcgtt caaccacctt ctgccaggag gcttcccgcc caccggcatg cccacgctgc 
     3361 ccccctacca gctgccggac tccacctacc ccaccaccac catctcccaa gatgggggca 
     3421 gcactgtgca ccggcctcag cccctgccac cgtccaccat gcaccagggc gggctggctg 
     3481 cagcggctgc agccgccgac accagctctg cctacggagc ccgccacagc ttctccagct 
     3541 actctgacag cttcatgaat ccggcggcgc cctccaacca catgaacccg gtcagcaacg 
     3601 gcctgtctcc tcaggtgatg agcatcttgg gcaaccccag tgcggtgccc ccgcagccac 
     3661 aggctgactt ctccatctcc ccgctgcatg gcggcctgga ctcggccacc tccatctcag 
     3721 ccagctgcag ccagcgggcc gactccatca agccaggaga cagcctgccc acctcccagg 
     3781 cctactgccc acccacctac agcaccaccg gctacagcgt ggaccccgtg gccggctatc 
     3841 agtacggcca gtacggccag agtgagtgcc tggtgccctg ggcgtccccc gtccccattc 
     3901 cttctcccac ccccagggcc tcctgcttgt ttatggagag ctacaaggtg gtgtcagggt 
     3961 ggggaatgtc catttcacag atggaaaaat tgaagtccag ccagatggaa cagttcacct 
     4021 aactcgagtt cgaaaacgga tctccgcggc cccgaattcc gccctctccc tccccccccc 
     4081 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 
     4141 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 
     4201 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 
     4261 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 
     4321 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 
     4381 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 
     4441 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 
     4501 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt 
     4561 agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa 
     4621 aacacgatga taagcttgcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg 
     4681 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     4741 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 
     4801 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 
     4861 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     4921 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     4981 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     5041 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 
     5101 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 
     5161 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 
     5221 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 
     5281 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 
     5341 tcggcatgga cgagctgtac aagtaaggga tcatcgctag caccggtctg cagtctagag 
     5401 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 
     5461 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 
     5521 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 
     5581 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 
     5641 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 
     5701 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     5761 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 
     5821 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 
     5881 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 
     5941 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 
     6001 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 
     6061 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 
     6121 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 
     6181 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 
     6241 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 
     6301 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 
     6361 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 
     6421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     6481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 
     6541 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     6601 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     6661 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg 
     6721 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 
     6781 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 
     6841 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc 
     6901 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     6961 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     7021 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     7081 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     7141 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     7201 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     7261 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     7321 aaaatattaa cgtttacaat ttcccaggtg gcacttttcg gggaaatgtg cgcggaaccc 
     7381 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     7441 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     7501 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     7561 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     7621 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     7681 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
     7741 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
     7801 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     7861 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     7921 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     7981 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     8041 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     8101 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     8161 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
     8221 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     8281 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     8341 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     8401 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     8461 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     8521 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     8581 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     8641 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     8701 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     8761 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     8821 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     8881 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     8941 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     9001 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     9061 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     9121 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     9181 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 
     9241 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 
     9301 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 
     9361 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 
     9421 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 
     9481 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac 
     9541 aaaagctgga gctgca 
//