LOCUS       [FC162]_htpG1_promoter_ 2844 bp ds-DNA     circular     17-SEP-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(2073..2836)
                     /label="ColE1"
     CDS             118..834
                     /label="sfGFP"
     misc_feature    14..73
                     /label="htpG1 promoter"
     RBS             82..117
                     /label="sfGFP Ribosome Binding Site"
     protein_bind    968..973
                     /label="BsaI"
     CDS             complement(1084..1941)
                     /label="AmpR"
     protein_bind    975..978
                     /label="BsaI"
     promoter        complement(1942..2072)
                     /label="AmpR Promoter"
     terminator      complement(987..1083)
                     /label="AmpR Terminator"
     terminator      835..963
                     /label="BBa_B0015 Terminator"
ORIGIN
        1 GGCCGCCTAG GCCacgagag caggatcacc tgctctcgct tgaaattatt ctcccttgtc
       61 cccatctctc ccaTTAATTA Aggatcttag ctactagaga aagaggagaa atactagatg
      121 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
      181 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
      241 ctgacgctga agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta
      301 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
      361 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
      421 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
      481 cgcattgagc tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg
      541 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
      601 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
      661 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
      721 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
      781 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 ataagtcggt ctcatacagc ggccgcgatt atcaaaaagg atcttcacct agatcctttt
     1021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1201 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
     1261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1981 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2041 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     2101 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2161 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2221 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2281 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2341 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2401 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2461 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2521 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2581 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2641 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2701 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2761 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2821 ttctgtggat aaccgtgcgg ccgc
//