LOCUS       Exported                7019 bp ds-DNA     circular SYN 22-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGW001_pAAV-U6-P53-U6sgBBSapI-TBG-Fluc-2A-Cre-spA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 22, 2018 from SnapGene Viewer 3.3.3
            http://www.snapgene.com
COMMENT     LOCUS pAAV-EF1a-EYFP-W 6067 bp DNA circular 20-MAY-2010 SOURCE 
            ORGANISM COMMENT VNTDATE|538328968| COMMENT VNTDBDATE|538763081| 
            COMMENT VNTNAME|pAAV-EF1a-EYFP-WPRE| COMMENT VNTAUTHORNAME|William 
            Hendriks| COMMENT VNTAUTHOREML|hendriks.william@mgh.harvard.edu| 
            COMMENT VNTAUTHORNAME|Feng Zhang| COMMENT 
            VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            1..141
                     /note="L-ITR"
     misc_feature    150..155
                     /note="MluI"
     promoter        156..404
                     /note="Human U6 Promoter"
     misc_feature    387..414
                     /note="pGW01-R1"
     misc_feature    397..430
                     /note="pCM102-103_F3"
     misc_feature    405..506
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    405..424
                     /note="Untitled"
     misc_feature    480..519
                     /note="pCM102-103_F1"
     promoter        513..762
                     /note="Human U6 Promoter"
     misc_feature    513..538
                     /note="Untitled"
     misc_feature    729..763
                     /note="Readout_priming_site_long_F"
     misc_feature    740..763
                     /note="Readout_priming_site_F"
     misc_feature    744..762
                     /note="Untitled"
     misc_feature    763..862
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    764..770
                     /note="SapI"
     misc_feature    773..779
                     /note="SapI"
     misc_feature    781..783
                     /note="3'"
     misc_feature    806..1445
                     /note="gBlock-GW"
     misc_feature    869..1370
                     /note="hTBG promoter"
     CDS             1389..3038
                     /note="Fluc"
     misc_feature    1585..1624
                     /note="pCM102_R6"
     misc_feature    3045..3110
                     /note="GSG-P2A"
     CDS             3111..4157
                     /note="Cre"
     CDS             4158..4184
                     /note="HA"
     misc_feature    4206..4253
                     /note="shortPA"
     gene            4282..4422
                     /note="R-ITR"
     exon            4514..4820
                     /note="f1 Origin"
     exon            5339..6196
                     /note="AmpR"
     rep_origin      6347..7014
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
      181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
      361 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgtgtaa tagctcctgc
      421 atgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      481 aagtggcacc gagtcggtgc tttttttcta gaagagggcc tatttcccat gattccttca
      541 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
      601 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      661 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      721 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggaagagc gagctcttct
      781 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      841 ggcaccgagt cggtgctttt ttggtaccgc ggcctctaga ctcgaggggc tggaagctac
      901 ctttgacatc atttcctctg cgaatgcatg tataatttct acagaaccta ttagaaagga
      961 tcacccagcc tctgcttttg tacaactttc ccttaaaaaa ctgccaattc cactgctgtt
     1021 tggcccaata gtgagaactt tttcctgctg cctcttggtg cttttgccta tggcccctat
     1081 tctgcctgct gaagacactc ttgccagcat ggacttaaac ccctccagct ctgacaatcc
     1141 tctttctctt ttgttttaca tgaagggtct ggcagccaaa gcaatcactc aaagttcaaa
     1201 ccttatcatt ttttgctttg ttcctcttgg ccttggtttt gtacatcagc tttgaaaata
     1261 ccatcccagg gttaatgctg gggttaattt ataactaaga gtgctctagt tttgcaatac
     1321 aggacatgct ataaaaatgg aaagataccg gtgccaccat ggccccaaag gttaaccgta
     1381 cggccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
     1441 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     1501 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
     1561 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     1621 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     1681 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     1741 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
     1801 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
     1861 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     1921 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
     1981 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
     2041 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     2101 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     2161 gtggatttcg agtcgtctta atgtatagat ttgaagagga gctgtttctg aggagccttc
     2221 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     2281 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     2341 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     2401 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     2461 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     2521 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     2581 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     2641 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     2701 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     2761 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     2821 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     2881 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     2941 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     3001 tcctcataaa ggccaagaag ggcggaaaga tcgccgtggc tagcggaagc ggagccacta
     3061 acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca cccaagaaga
     3121 agaggaaggt gtccaatctc ctgactgttc accagaacct ccctgcgctg ccagtagatg
     3181 ccactagcga tgaggtcagg aaaaatctca tggatatgtt tagggataga caggcgtttt
     3241 ctgaacacac ctggaaaatg ctgcttagcg tgtgccgatc ctgggcagcc tggtgtaagc
     3301 tgaacaatcg caaatggttc cccgccgagc cggaggacgt gcgcgattac ctgctgtatc
     3361 tccaggcaag agggctggct gtcaagacta tccagcagca cttgggccaa ctgaatatgc
     3421 tgcatcgacg cagcgggctc ccccggccta gcgattcaaa cgcagtctcc cttgttatga
     3481 ggagaattag aaaggaaaac gtagatgcgg gtgagagggc taagcaggct ctcgcttttg
     3541 agcggactga tttcgaccag gtcagatccc tgatggagaa cagcgatcgg tgccaggaca
     3601 tcaggaacct cgcatttctg ggaattgcat ataacacact tctgcgcata gctgagatcg
     3661 cccggatcag agtgaaagac atcagtcgaa cggacggcgg ccggatgctt attcatattg
     3721 gacgcacaaa gacattggtc agcaccgctg gcgttgaaaa ggccttgtcc ctgggcgtaa
     3781 cgaagctggt ggaaagatgg atctcagtgt ccggcgtggc tgacgaccct aataattact
     3841 tgttctgtcg agtgagaaaa aacggagtcg ccgcgccctc tgccaccagc caattgagta
     3901 cacgggccct tgaagggatc tttgaggcaa cccaccgact catatacgga gccaaggatg
     3961 acagtggcca gaggtatctc gcctggtcag gtcattctgc tagggtgggg gccgcacgag
     4021 acatggcgcg ggcaggagtc tccataccag agattatgca agctggaggt tggacaaatg
     4081 tgaacatcgt tatgaactat atccgcaatc ttgactctga aaccggggcc atggtgagac
     4141 tgctcgaaga tggtgactac ccatacgatg ttccagatta cgcttaagaa ttcgatatca
     4201 agcttaataa aagatcttta ttttcattag atctgtgtgt tggttttttg tgtggtaacc
     4261 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4321 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4381 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4441 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4501 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4561 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4621 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4681 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     4741 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4801 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     4861 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4921 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     4981 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5041 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5101 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5161 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5221 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5281 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5341 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5401 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5461 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5521 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5581 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5641 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5701 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5761 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5821 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5881 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5941 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6001 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6061 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6121 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6181 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6241 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6301 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6361 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6421 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6481 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6541 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6601 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6661 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6721 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6781 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6841 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6901 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6961 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
