LOCUS       pRSET-B	2887 bp	DNA	SYN	14-Mar-2018
DEFINITION  pRSET-B
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     promoter        20..38
                     /label=T7_promoter
     misc_feature    75..91
                     /label=T7_transl_en_RBS
     terminator      992..1120
                     /label=T7_terminator
     rep_origin      1209..1515
                     /label=f1_origin
     promoter        1708..1736
                     /label=AmpR_promoter
     gene            1778..2638
                     /label=beta lactamase
                     /gene="beta lactamase"
     rep_origin      2793..3412
                     /label=pBR322_origin
     misc_feature    952..960
                     /note="3xSTOP"
     misc_feature    184..207
                     /label=Xpress_EK
     misc_feature    184..222
                     /note=""
     misc_feature    112..141
                     /note="10 x His"
     gene            220..951
                     /note="mCarmine"
     misc_feature    100..219
                     /note="purification tag"
     source          1..3633
                     /dnas_title="mCarmine_pRSETSLIII"
ORIGIN      
        1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT
       61 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT
      121 CATCATCACC ATCACCATCA CCGCGGTATG GCTAGCATGA CTGGTGGACA GCAAATGGGT
      181 CGGGATCTGT ACGACGATGA CGATAAGGAT CCGgccacca tgGTGTCTAA GGGCGAAGAG
      241 CTGATTAAGG AGAACATGCA CATGAAGCTG TACATGGAGG GCACCGTGAA CAACCACCAC
      301 TTCAAGTGCA CATCCGAGGG CGAAGGCAAG CCCTACGAGG GCACCCAGAC CCAGAGAATC
      361 AAGGTGGTCG AGGGCGGCCC TCTCCCCTTC GCCTTCGACA TCCTGGCTAC CaGCTTCATG
      421 TACGGCAGCA AGACCTTCAT CAACCACACC CAGGGCATCC CCGATTTCTT TAAGCAGTCC
      481 TTCCCTGAGG GCTTCACATG GGAGAGAGTC ACCACATACG AAGACGGGGG CGTGCTGAAG
      541 GTGAAACAGG ACACCAGCCT CCAGGACGGC TGCCTCATCT ACAACGTCAA GATCAGAGGG
      601 GTGAACTTCC CATCCAACGG CCCTGTGATG CAGAAGAAAA CACTCGGCTG GGAGGCCAAC
      661 ACCGAGACCC TGTACCCCGC TGACGGCGGC CTGCGCGGCT ATAACACGAT GGCCCTGAAG
      721 CTCGTGGGCG GGGGCCACCT GCAGACGTTC CTCAAGACCA CATACAGATC CAAGAAACCC
      781 GCTAAGAACC TCAAGATGCC CGGCGTCTAC TTTGTGGACC GCAGACTGGA AAGAATCAAG
      841 GAGGCCGACA AAGAGACCTA CGTCGAGCAG CACGAGGTGG CTGTGGCCAG ATACTGCGAC
      901 CTCCCTAGCA AACTGGGGCA CAAACTTAAT GGCATGGACG AGCTGTACAA GTGATAATAG
      961 GAATTCctat agtgtcacct aaatGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG
     1021 TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1081 TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT CTGGCGTAAT AGCGAAGAGG
     1141 CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT
     1201 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG
     1261 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG
     1321 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC
     1381 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT
     1441 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT
     1501 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT
     1561 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT
     1621 TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
     1681 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
     1741 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
     1801 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
     1861 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
     1921 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
     1981 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
     2041 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
     2101 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
     2161 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
     2221 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
     2281 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT
     2341 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
     2401 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
     2461 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG
     2521 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
     2581 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT
     2641 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
     2701 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
     2761 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     2821 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     2881 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     2941 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     3001 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     3061 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     3121 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     3181 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     3241 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     3301 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     3361 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     3421 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
     3481 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
     3541 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC
     3601 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAG
//

