LOCUS pMINTC3GH_(Hyg) 9171 bp DNA circular UNA 08-Okt-2013 DEFINITION SOURCE pMINT_C3GH (unknown) FEATURES Location/Qualifiers Fragment join(<4009..8895,2027..>4008,8896..9171,1..352) /origin="pMINT_C3GH dccdB (3) [#25] (7'857bp circular)Fragment: from SapI cut at 3237^3238 to SapI cut at 1939^1940, 6'559bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" promoter 154..216 /label="P(myc1)" Fragment 353..2026 /origin="ccdB CM (2'780bp linear)Fragment: from SapI cut at 130^131 to SapI cut at 1804^1805, 1'674bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" CDS complement(357..363) /label="SapI" gene complement(401..706) /gene="ccdB" /product="ccdB gene" misc_feature complement(806..834) /gene="-8.0" gene complement(1048..1707) /gene="cat" /product="Chloramphenicol resistance gene" CDS 2019..2025 /label="SapI" CDS 2030..2053 /label="C3" gene 2054..2809 /label="GFP-His10" oriT 3038..3657 /label="pBR322 OriE" gene 4430..5081 /label="Hyg" gene complement(6007..7005) /label="Integrase" CDS complement(7083..7585) /label="attP" gene complement(8083..8892) /label="TetR(B_syn207)" promoter complement(8902..9045) /label="P(imyc)" ORIGIN 1 GTCGGCACCG TCACGGCCGT GGGAGGCGGC ACGATCCGCG ACGTGATGAT CGGCCGCATC 61 CCCACGGTGC TGCGCAGTGA GCTCTACGCC ATCCCGGCGT TGATCTGTGC GTTCGCACGC 121 ACAGGCCCGG TGTGAGAAGG GTCTCTGACG AGCGGGAGAA CTCCCTATCA GTGATAGAGT 181 TTGTCCTCCC TATCAGTGAT AGATAGGCTC TGGGAGTACC CGTGTGTACG ACCAGCACGG 241 CATACATCAT TTCGACGCCG AGAGATTCGC CGCCCGAAAT GAGCACGATC CGCATGCACC 301 CAGCTTTCTT GTACAAAGTG GGCTCGAGGC ATGCGAAGGA GATATACATA TGAGTTGAAG 361 AGCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA 421 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA 481 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA 541 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA 601 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA 661 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG 721 TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC 781 TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG 841 CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT 901 CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA 961 CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT 1021 GGCTTTTAGT AAGCCGGATC CACGCGTTTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG 1081 TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG 1141 AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC 1201 GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA 1261 GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT 1321 TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG 1381 GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG 1441 GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ATTCCGGATG 1501 AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT 1561 CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG 1621 AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT 1681 GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGACGG 1741 ATCCTAACTC AAAATCCACA CATTATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 1801 GCCTAATGCG GCCGCCAATA TGACTGGATA TGTTGTGTTT TACAGTATTA TGTAGTCTGT 1861 TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT TTCTCGTTCA 1921 GCTTTTTTGT ACAAACTTGT GATATCGAAT TCGCTAGCTA CCTTTCTCTT CTTTTTTGGA 1981 TCTACCTTTC TCTTCTTTTT TGGGGATCCA CTAGTTCTGC TCTTCTGCAT TAGAAGTTTT 2041 GTTTCAAGGT CCACAATTCA GCAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT 2101 TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG 2161 TGATGCTACA TACGGAAAAC TCACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT 2221 TCCTTGGCCA ACACTTGTCA CTACTCTGAC CTATGGTGTT CAATGCTTTT CCCGTTATCC 2281 GGATCACATG AAACGGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA 2341 ACGCACTATA TCTTTCAAAG ATGACGGGAA CTACAAGACG CGTGCTGAAG TCAAGTTTGA 2401 AGGTGATACC CTTGTTAATC GTATCGAGTT AAAGGGTATT GATTTTAAAG AAGATGGAAA 2461 CATTCTCGGA CACAAACTAG AGTACAACTA TAACTCACAC AATGTATACA TCACGGCAGA 2521 CAAACAAAAG AATGGAATCA AAGCTAACTT CAAAATTCGC CACAACATTG AAGATGGTTC 2581 CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT 2641 ACCAGACAAC CATTACCTGT CGACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGCG 2701 TGACCACATG GTCCTTCTTG AGTTTGTAAC TGCTGCTGGG ATTACACATG GCATGGATGA 2761 GCTCTACAAA ACTAGTCATC ATCATCACCA TCATCATCAC CATCATTAAT TATACATAGT 2821 TGCTTAAGCT GCAGGGGACC CTAGAGGTCC CCTTTTTTAT TTTAAAAATT TTTTATAACC 2881 GGTAAGAAGT CCAACACGCC GCTCCTCCCC GATGTTGCGT AGCCGCTTCA AGCTTAAGCA 2941 ACGGCCCCAG TGAGTAGCGA CTTTGGATCC GGTACCCGCA ACGACTGGCC GACATGTGAG 3001 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 3061 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 3121 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 3181 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 3241 TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 3301 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 3361 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 3421 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 3481 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 3541 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 3601 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 3661 CTACGGGGTC TGACGCTCAG TGGAACTAGT GAGGTCTGCC TCGTGAAGAA GGTGTTGCTG 3721 ACTCATACCA GGCCTGAATC GCCCCATCAT CCAGCCAGAA AGTGAGGGAG CCACGGTTGA 3781 TGAGAGCTTT GTTGTAGGTG GACCAGTTGG TGATTTTGAA CTTTTGCTTT GCCACGGAAC 3841 GGTCTGCGTT GTCGGGAAGA TGCGTGATCT GATCCTTCAA CTCAGCAAAA GTTCGATTTA 3901 TTCAACAAAG CCGCCGTCCC GTCAAGTCAG CGTAATGCTC TGCCAGTGTT ACAACCAATT 3961 AACCAATTCT GATTAGAAAA ACTCATCGAG CATCAAATGA AACTGCAAGT ATTCTAGAGG 4021 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 4081 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 4141 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 4201 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG ACGTGGCCGA CCAGCCCGTC 4261 ATCGTCAACG CCTGATCCGC GGTGCGGACA GGCCGTGTCG CGACCGGCCG TGCGGAATTA 4321 AGCCGGCCCG TACCCTGTGA ATAGAGGTCC GCTGTGACAC AAGAATCCCT GTTACTTCTC 4381 GACCGTATTG ATTCGGATGA TTCCTACGCG AGCCTGCGGA ACGACCAGGA ATTCTGGGAG 4441 CCGCTGGCCC GCCGAGCCCT GGAGGAGCTC GGGCTGCCGG TGCCGCCGGT GCTGCGGGTG 4501 CCCGGCGAGA GCACCAACCC CGTACTGGTC GGCGAGCCCG GCCCGGTGAT CAAGCTGTTC 4561 GGCGAGCACT GGTGCGGTCC GGAGAGCCTC GCGTCGGAGT CGGAGGCGTA CGCGGTCCTG 4621 GCGGACGCCC CGGTGCCGGT GCCCCGCCTC CTCGGCCGCG GCGAGCTGCG GCCCGGCACC 4681 GGAGCCTGGC CGTGGCCCTA CCTGGTGATG AGCCGGATGA CCGGCACCAC CTGGCGGTCC 4741 GCGATGGACG GCACGACCGA CCGGAACGCG CTGCTCGCCC TGGCCCGCGA ACTCGGCCGG 4801 GTGCTCGGCC GGCTGCACAG GGTGCCGCTG ACCGGGAACA CCGTGCTCAC CCCCCATTCC 4861 GAGGTCTTCC CGGAACTGCT GCGGGAACGC CGCGCGGCGA CCGTCGAGGA CCACCGCGGG 4921 TGGGGCTACC TCTCGCCCCG GCTGCTGGAC CGCCAGGAGG ACTGGCTGCC GGACGTGGAC 4981 ACGCTGCTGG CCGGCCGCGA ACCCCGGTTC GTCCACGGCG ACCTGCACGG GACCAACATC 5041 TTCGTGGACC TGGCCGCGAC CGAGGTCACC GGGATCGTCG ACTTCACCGA CGTCTATGCG 5101 GGAGACTCCC GCTACAGCCT GGTGCAACTG CATCTCAACG CCTTCCGGGG CGACCGCGAG 5161 ATCCTGGCCG CGCTGCTCGA CGGGGCGCAG TGGAAGCGGA CCGAGGACTT CGCCCGCGAA 5221 CTGCTCGCCT TCACCTTCCT GCACGACTTC GAGGTGTTCG AGGAGACCCC GCTGGATCTC 5281 TCCGGCTTCA CCGATCCGGA GGAACTGGCG CAGTTCCTCT GGGGGCCGCC GGACACCGCC 5341 CCCGGCGCCT GACGCCCCGG GCCGCCCGGC GCCGCCCCCG GCCCCCGGCG GCCGCCGGGC 5401 GGAGCCCCGC CCGGCGGCCC GGGAGCCCCG GGCCCGCGCC GAAGCCCGCT GCTGCGAGCC 5461 CGGAGCGGGC CGGCCGACGG CGGTGCGGGC CCGGCGGCGG ACGCTCAGCA GCGGCGGGCG 5521 TGAAAGGCCC TGGCATCCTC GATCATCTCC TCCAGGGTGG GTCGGCCNGG GCTTCCATCC 5581 CAGCTCGGCA AGGATCAACG AGGACAGTCG CACGACGAAG TTCTTCTGGA TCGCGCCCGT 5641 GCTGGAAGCA CTCAACCTCG AAGCGTGTGG TTGCGGAGCC ATCTAGCAAC CACACGACCA 5701 TGGTACTCCC GTTGAATATG GCTCATAACA CCCCTTGTAT TACTGTTTAT GTAAGCAGAC 5761 AGTTTTATTG TTCATGATGA TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA 5821 GACACAACGT CGCTTTGTTG CTAGCTGGCG GGAACGGCAA TGCTCCTGGC GGCGGCGGGG 5881 CTGGTGCCCA GGTCTCCGCA CAGAGCGGCG GTGCTGGCGC TCGCGGCCAG GCGTGGTTCT 5941 TCGCGTACTG ACAAGAAACC CCCCTCTTTA GGACTCAGTG TCCTTGGGAG GGGGGCTTTT 6001 TGCGTTTCAG GAGGTCTTGG CCAGCTTGGA CATCGCCTCA GCGATAGCCT CGTCGCGGGC 6061 CTCAGACGCC ATCTGGTACT TCATCGCCAT CCTAGGAGTC GTGTGACCGA GACGGGCCAT 6121 CAGCTCCTTG GTCGTCGCAC CTGCCTGAGC GGCGAACGTA GCGCCGACAG CGCGGAGGTC 6181 GTGGATGCGG AGTTCCGGCC GACCGATCTT GGCGTAGCCA CGCTTCAGCG ACTTGGTGAA 6241 CGCGGACTTC GACAGCCGGT TGCCCTGCGT CGTGGTCACC AGGAATGCCT CGGGGCCCTT 6301 GTTCATCTTC GTACGGTCCT TCATGTGCGC TCGGATCATC TCCGCGACGT GAGGCGGAAC 6361 CGTCACAGGA CGCTTCGACC GGACGGTCTT GGCGTTGCCA ACGACGATCT TGTTCCCCAC 6421 GCGGGAAGCG CCACGGCGCA CCCGGAGCTT CATCGTCATG CCGTCGTCCA CGATGTCCTT 6481 GCGGCGAAGC TCGATCAGCT CTCCGAACCG GAGGCTCGTC CACGCCAGGA TGTATGCCGC 6541 GATCCGGTAG TGCTCGAAGA TCTCAGCGGC GACGATGTCC AGCTCCTCAG GCGTCAGCGC 6601 CTCTACGTCG CGCTCATCGG CTGCCTTCTG CTCGATCCGG CACGGGTTCT CTGCGATCAG 6661 CTTGTCCTCG ACCGCTGTGT TCATCACCGC CCGGAGGACG TTGTAGGCAT GCCGGCGGGC 6721 AGTCGGGTGC TTCCTACCCA TCCCGGCCCA CCACGCACGC ACCAGAGCTG GCGTCATCTC 6781 TGTGACCGCC ACTTCACCTA GCACCGGGTA GATGCGGCGC TCCGCGTGCC CGCTGTACAG 6841 ATCCCTGGTG CCGTCTGCGA GGTCGCGCTC CACGAGCCAC TTCCGGGTGT ACTCCTCCAG 6901 CGTGATGGCG CTGGCGGCTG CCTTCTTCGC CCGGTCCTGT GGAGGGGTCC AGGTCTCCAT 6961 CTCGATGAGC CGCTTCTCGC CCGCGAGCCA GGCTTCGGCG TCCATCTTGT TGTCGTAGGT 7021 CTGCAGCGCG TAGTACCTCA CACCGTCCTG CGGGTTGACG TATGAGGCTT GGATCCTCCC 7081 GCTGCGCTGA GTCTTCAGCG ATCCCCATCC GCGACGTGCC AACTAGGTCT CCTCTCGTCG 7141 TGAACAAGGC TACCGGGTTG CAACTCCTGT GCAACTCTCA GGCTTCAACG CGCTTCTACG 7201 ACCTGCAATT TCTTTCCACT TAGAGGATGC AGCCGAGAGG GGGTAAAAAC CTATCTTGAC 7261 CGGCCCATAT ATGTGGTCGG CAGACACCCA TTCTTCCAAA CTAGCTACGC GGGTTCGATT 7321 CCCGTCGCCC GCTCCGCTGG TCAGAGGGTG TTTTCGCCCT CTGGCCATTT TTCTTTCCAG 7381 GGGTCTGCAA CTCTTGTGCG ACTCTTCTGA CCTGGGCATA CGCGGTTGCA ACGCATCCCT 7441 GATCTGGCTA CTTTCGATGC TGACAAACGA ATAGAGCCCC CCGCCTGCGC GAACAGACGA 7501 GGGGCATTCA CACCAGATTG GAGCTGGTGC AGTGAAGAGA ATAGACCGGG ACAAGGTTGC 7561 ACCGGGAGTT GCAGCGGTCG GAACCCTCGC CGTCGGCGGG CTGGCGTTCG CCCTGTCGTT 7621 CACGGCTCTA GCTGATCACC GCGGCCATGA TGGCCGGACA AACAACAGAT AAAACGAAAG 7681 GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC TGGCAGTCGA TCGTACGCTA 7741 GTTAACTACG TCGACATCGA TTTAATTAAT ATGCATCAAT TGATTTAAAT ACTAGTGTTA 7801 ACTATTTAAT TGGGGACCCT AGAGGTCCCC TTTTTTATTT TAAAAATTTT TTCNAAGGGG 7861 ACCTCTAGGG TCCCCAGCTG GCTAGCGAGT CATGAGGTNA CCATGTTCTT TCCTGCGTTA 7921 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CAGCGGATAA 7981 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTATCAAC TTTGTATAGA 8041 AAAGTTGCAT GACCAACTTC GATAACGTTC TCGGCTCGAT GATCCCCGGC GGGCCCCCCC 8101 TCGAGGTCGA CGGTATCGAT TTAATTAATA TGCATCAATT GATTTAAATA CTAGTGTTAA 8161 CTATTTAATT GGGGACCCTA GAGGTCCCCT TTTTTATTTT AAAAATTTTT TCNAAGGGGA 8221 CCTCTAGGGT CCCCAGCTGG CTAGCGAGTC ATGAGGTNAC CATGGTCATT AGGAGCCGCT 8281 CTCGCACTTC AGCTGCTTCT CCAAGCCGCA GATGATCAGC TCCAGGCCGA ACAAGAAGGC 8341 CGGCTCGGCG CCCTGGTGGT CGAACAGCTC GATGGCCTGG CGCAGCAACG GCGGCATGCT 8401 GTCGGTGGTC GGGGTCTCGC GCTCCTCCTT GGCGACCTGG TGCTCCTGGT CCTCCAACAC 8461 GCAGCCCAGG GTGAAGTGGC CCACGGCCGA CAGGGCGTAC AACGCGTTCT CCAGCGAGAA 8521 GCCCTGCTGG CACAGGAAGG CCAACTGGTT CTCCAGGGTC TCGTACTGCT TCTCGGTCGG 8581 CCGGGTGCCC AGGTGCACCT TGGCGCCGTC GCGGTGGCTC AGCAAGGCGC AGCGGAAGCT 8641 CTTGGCGTTG TTGCGCAGGA AGTCCTGCCA GCTCTCGCCC TCCAGCGGGC AGAAGTGGGT 8701 GTGGTGGCGG TCCAACATCT CGATGGCCAG GGCGTCGAGC AGGGCCCGCT TGTTCTTGAC 8761 GTGCCAGTAC AGGGTCGGCT GCTCGACGCC CAGCTTCTGG GCCAGCTTGC GGGTGGTCAG 8821 GCCCTCGATG CCGACCTCGT TCAGCAGCTC CAGGGCGGAG TTGATGACCT TGGACTTGTC 8881 CAGGCGGGAC ATTGCGAAGT GATTCCTCCT GATGGTACCG GATCCGTGGC AGGAGGGTCG 8941 CCTGCGTCGG CAACTCGCCC AGGCTACACG AGAGCCCTCG ACTGCGAAAA TGCCGACCAG 9001 GCACCACCGC ACTCCAGGCT TAACCCGGTG CGACGATCCA ATATTACGAG ATCTGACAAG 9061 TTTGTACAAA AAAGCAGGCT ACCAGGCCTA GATCTGGGGA CCCTAGAGGT CCCCTTTTTT 9121 ATTTTAAAAA TTTTTTCACG TGCCAGCCGA TTGGACTAGA AATATTGGAT C //