LOCUS       pMINTC3GH_(Apr)      8580 bp    DNA     circular UNA 08-Okt-2013
DEFINITION  
SOURCE      pMINT_C3GH  (unknown)
FEATURES             Location/Qualifiers
     Fragment        join(<3965..8260,1983..>3964,8261..8580,1..308)
                     /origin="pMINT_C3GH dccdB (3) [#25] (7'857bp
                     circular)Fragment: from SapI cut at 3237^3238 to SapI cut
                     at 1939^1940, 6'559bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        110..172
                     /label="P(myc1)"
     Fragment        309..1982
                     /origin="ccdB CM (2'780bp linear)Fragment: from SapI cut
                     at 130^131 to SapI cut at 1804^1805, 1'674bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     CDS             complement(313..319)
                     /label="SapI"
     gene            complement(357..662)
                     /gene="ccdB"
                     /product="ccdB gene"
     misc_feature    complement(762..790)
                     /gene="-8.0"
     gene            complement(1004..1663)
                     /gene="cat"
                     /product="Chloramphenicol resistance gene"
     CDS             1975..1981
                     /label="SapI"
     CDS             1986..2009
                     /label="C3"
     gene            2010..2765
                     /label="GFP-His10"
     oriT            2994..3613
                     /label="pBR322 OriE"
     gene            4117..4893
                     /label="Apr"
     gene            complement(5372..6370)
                     /label="Integrase"
     CDS             complement(6448..6950)
                     /label="attP"
     gene            complement(7448..8257)
                     /label="TetR(B_syn207)"
     promoter        complement(8267..8410)
                     /label="P(imyc)"
ORIGIN
        1 GATGATCGGC CGCATCCCCA CGGTGCTGCG CAGTGAGCTC TACGCCATCC CGGCGTTGAT
       61 CTGTGCGTTC GCACGCACAG GCCCGGTGTG AGAAGGGTCT CTGACGAGCG GGAGAACTCC
      121 CTATCAGTGA TAGAGTTTGT CCTCCCTATC AGTGATAGAT AGGCTCTGGG AGTACCCGTG
      181 TGTACGACCA GCACGGCATA CATCATTTCG ACGCCGAGAG ATTCGCCGCC CGAAATGAGC
      241 ACGATCCGCA TGCACCCAGC TTTCTTGTAC AAAGTGGGCT CGAGGCATGC GAAGGAGATA
      301 TACATATGAG TTGAAGAGCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT
      361 ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA
      421 GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC
      481 CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC
      541 AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC
      601 TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC
      661 ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC
      721 CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC
      781 ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC
      841 TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG
      901 ACATACTTCG GGTATACATA TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT
      961 ACGCATACTG TTATCTGGCT TTTAGTAAGC CGGATCCACG CGTTTACGCC CCGCCCTGCC
     1021 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA
     1081 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT
     1141 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC
     1201 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG
     1261 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT
     1321 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA
     1381 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA
     1441 TACGGAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA
     1501 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT
     1561 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT
     1621 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC
     1681 CTGAAAATCT CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA
     1741 GTGTAAAGCC TGGGGTGCCT AATGCGGCCG CCAATATGAC TGGATATGTT GTGTTTTACA
     1801 GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT
     1861 TTACGTTTCT CGTTCAGCTT TTTTGTACAA ACTTGTGATA TCGAATTCGC TAGCTACCTT
     1921 TCTCTTCTTT TTTGGATCTA CCTTTCTCTT CTTTTTTGGG GATCCACTAG TTCTGCTCTT
     1981 CTGCATTAGA AGTTTTGTTT CAAGGTCCAC AATTCAGCAA AGGAGAAGAA CTTTTCACTG
     2041 GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA
     2101 GTGGAGAGGG TGAAGGTGAT GCTACATACG GAAAACTCAC CCTTAAATTT ATTTGCACTA
     2161 CTGGAAAACT ACCTGTTCCT TGGCCAACAC TTGTCACTAC TCTGACCTAT GGTGTTCAAT
     2221 GCTTTTCCCG TTATCCGGAT CACATGAAAC GGCATGACTT TTTCAAGAGT GCCATGCCCG
     2281 AAGGTTATGT ACAGGAACGC ACTATATCTT TCAAAGATGA CGGGAACTAC AAGACGCGTG
     2341 CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATCGTAT CGAGTTAAAG GGTATTGATT
     2401 TTAAAGAAGA TGGAAACATT CTCGGACACA AACTAGAGTA CAACTATAAC TCACACAATG
     2461 TATACATCAC GGCAGACAAA CAAAAGAATG GAATCAAAGC TAACTTCAAA ATTCGCCACA
     2521 ACATTGAAGA TGGTTCCGTT CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG
     2581 ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCGAC ACAATCTGCC CTTTCGAAAG
     2641 ATCCCAACGA AAAGCGTGAC CACATGGTCC TTCTTGAGTT TGTAACTGCT GCTGGGATTA
     2701 CACATGGCAT GGATGAGCTC TACAAAACTA GTCATCATCA TCACCATCAT CATCACCATC
     2761 ATTAATTATA CATAGTTGCT TAAGCTGCAG GGGACCCTAG AGGTCCCCTT TTTTATTTTA
     2821 AAAATTTTTT ATAACCGGTA AGAAGTCCAA CACGCCGCTC CTCCCCGATG TTGCGTAGCC
     2881 GCTTCAAGCT TAAGCAACGG CCCCAGTGAG TAGCGACTTT GGATCCGGTA CCCGCAACGA
     2941 CTGGCCGACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     3001 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     3061 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     3121 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     3181 TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     3241 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     3301 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     3361 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     3421 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     3481 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     3541 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     3601 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACTAGTGAGG TCTGCCTCGT
     3661 GAAGAAGGTG TTGCTGACTC ATACCAGGCC TGAATCGCCC CATCATCCAG CCAGAAAGTG
     3721 AGGGAGCCAC GGTTGATGAG AGCTTTGTTG TAGGTGGACC AGTTGGTGAT TTTGAACTTT
     3781 TGCTTTGCCA CGGAACGGTC TGCGTTGTCG GGAAGATGCG TGATCTGATC CTTCAACTCA
     3841 GCAAAAGTTC GATTTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA ATGCTCTGCC
     3901 AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC AAATGAAACT
     3961 GCAAGTATCT AGAAGGATCT TCACCTAGAT CCTTTTGGTT CATGTGCAGC TCCATCAGCA
     4021 AAAGGGGATG ATAAGTTTAT CACCACCGAC TATTTGCAAC AGTGCCGTTG ATCGTGCTAT
     4081 GATCGACTGA TGTCATCAGC GGTGGAGTGC AATGTCGTGC AATACGAATG GCGAAAAGCC
     4141 GAGCTCATCG GTCAGCTTCT CAACCTTGGG GTTACCCCCG GCGGTGTGCT GCTGGTCCAC
     4201 AGCTCCTTCC GTAGCGTCCG GCCCCTCGAA GATGGGCCAC TTGGACTGAT CGAGGCCCTG
     4261 CGTGCTGCGC TGGGTCCGGG AGGGACGCTC GTCATGCCCT CGTGGTCAGG TCTGGACGAC
     4321 GAGCCGTTCG ATCCTGCCAC GTCGCCCGTT ACACCGGACC TTGGAGTTGT CTCTGACACA
     4381 TTCTGGCGCC TGCCAAATGT AAAGCGCAGC GCCCATCCAT TTGCCTTTGC GGCAGCGGGG
     4441 CCACAGGCAG AGCAGATCAT CTCTGATCCA TTGCCCCTGC CACCTCACTC GCCTGCAAGC
     4501 CCGGTCGCCC GTGTCCATGA ACTCGATGGG CAGGTACTTC TCCTCGGCGT GGGACACGAT
     4561 GCCAACACGA CGCTGCATCT TGCCGAGTTG ATGGCAAAGG TTCCCTATGG GGTGCCGAGA
     4621 CACTGCACCA TTCTTCAGGA TGGCAAGTTG GTACGCGTCG ATTATCTCGA GAATGACCAC
     4681 TGCTGTGAGC GCTTTGCCTT GGCGGACAGG TGGCTCAAGG AaAAGAGCCT TCAGAAGGAA
     4741 GGTCCAGTCG GTCATGCCTT TGCTCGGTTG ATCCGCTCCC GCGACATTGT GGCGACAGCC
     4801 CTGGGTCAAC TGGGCCGAGA TCCGTTGATC TTCCTGCATC CGCCAGAGGC GGGATGCGAA
     4861 GAATGCGATG CCGCTCGCCA GTCGATTGGC TGAGCTCATG AGCGGAGAAC GAGATGACGT
     4921 TGGAGGGGCA AGGTCGCGCT GATTGCTGGG GCAACACGTG GAGCGGATCG GGGATTGTCT
     4981 TTCTTCAGCT CGCTGATGAT ATGCTGACGC TCAATGCCGT TTGGCCTCCG ACTAACGAAA
     5041 ATCCCGCATT TGGACGGCTG ATCCATGGTA CTCCCGTTGA ATATGGCTCA TAACACCCCT
     5101 TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT GATGATATAT TTTTATCTTG
     5161 TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTCGCTT TGTTGCTAGC TGGCGGGAAC
     5221 GGCAATGCTC CTGGCGGCGG CGGGGCTGGT GCCCAGGTCT CCGCACAGAG CGGCGGTGCT
     5281 GGCGCTCGCG GCCAGGCGTG GTTCTTCGCG TACTGACAAG AAACCCCCCT CTTTAGGACT
     5341 CAGTGTCCTT GGGAGGGGGG CTTTTTGCGT TTCAGGAGGT CTTGGCCAGC TTGGACATCG
     5401 CCTCAGCGAT AGCCTCGTCG CGGGCCTCAG ACGCCATCTG GTACTTCATC GCCATCCTAG
     5461 GAGTCGTGTG ACCGAGACGG GCCATCAGCT CCTTGGTCGT CGCACCTGCC TGAGCGGCGA
     5521 ACGTAGCGCC GACAGCGCGG AGGTCGTGGA TGCGGAGTTC CGGCCGACCG ATCTTGGCGT
     5581 AGCCACGCTT CAGCGACTTG GTGAACGCGG ACTTCGACAG CCGGTTGCCC TGCGTCGTGG
     5641 TCACCAGGAA TGCCTCGGGG CCCTTGTTCA TCTTCGTACG GTCCTTCATG TGCGCTCGGA
     5701 TCATCTCCGC GACGTGAGGC GGAACCGTCA CAGGACGCTT CGACCGGACG GTCTTGGCGT
     5761 TGCCAACGAC GATCTTGTTC CCCACGCGGG AAGCGCCACG GCGCACCCGG AGCTTCATCG
     5821 TCATGCCGTC GTCCACGATG TCCTTGCGGC GAAGCTCGAT CAGCTCTCCG AACCGGAGGC
     5881 TCGTCCACGC CAGGATGTAT GCCGCGATCC GGTAGTGCTC GAAGATCTCA GCGGCGACGA
     5941 TGTCCAGCTC CTCAGGCGTC AGCGCCTCTA CGTCGCGCTC ATCGGCTGCC TTCTGCTCGA
     6001 TCCGGCACGG GTTCTCTGCG ATCAGCTTGT CCTCGACCGC TGTGTTCATC ACCGCCCGGA
     6061 GGACGTTGTA GGCATGCCGG CGGGCAGTCG GGTGCTTCCT ACCCATCCCG GCCCACCACG
     6121 CACGCACCAG AGCTGGCGTC ATCTCTGTGA CCGCCACTTC ACCTAGCACC GGGTAGATGC
     6181 GGCGCTCCGC GTGCCCGCTG TACAGATCCC TGGTGCCGTC TGCGAGGTCG CGCTCCACGA
     6241 GCCACTTCCG GGTGTACTCC TCCAGCGTGA TGGCGCTGGC GGCTGCCTTC TTCGCCCGGT
     6301 CCTGTGGAGG GGTCCAGGTC TCCATCTCGA TGAGCCGCTT CTCGCCCGCG AGCCAGGCTT
     6361 CGGCGTCCAT CTTGTTGTCG TAGGTCTGCA GCGCGTAGTA CCTCACACCG TCCTGCGGGT
     6421 TGACGTATGA GGCTTGGATC CTCCCGCTGC GCTGAGTCTT CAGCGATCCC CATCCGCGAC
     6481 GTGCCAACTA GGTCTCCTCT CGTCGTGAAC AAGGCTACCG GGTTGCAACT CCTGTGCAAC
     6541 TCTCAGGCTT CAACGCGCTT CTACGACCTG CAATTTCTTT CCACTTAGAG GATGCAGCCG
     6601 AGAGGGGGTA AAAACCTATC TTGACCGGCC CATATATGTG GTCGGCAGAC ACCCATTCTT
     6661 CCAAACTAGC TACGCGGGTT CGATTCCCGT CGCCCGCTCC GCTGGTCAGA GGGTGTTTTC
     6721 GCCCTCTGGC CATTTTTCTT TCCAGGGGTC TGCAACTCTT GTGCGACTCT TCTGACCTGG
     6781 GCATACGCGG TTGCAACGCA TCCCTGATCT GGCTACTTTC GATGCTGACA AACGAATAGA
     6841 GCCCCCCGCC TGCGCGAACA GACGAGGGGC ATTCACACCA GATTGGAGCT GGTGCAGTGA
     6901 AGAGAATAGA CCGGGACAAG GTTGCACCGG GAGTTGCAGC GGTCGGAACC CTCGCCGTCG
     6961 GCGGGCTGGC GTTCGCCCTG TCGTTCACGG CTCTAGCTGA TCACCGCGGC CATGATGGCC
     7021 GGACAAACAA CAGATAAAAC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG
     7081 ATGCCTGGCA GTCGATCGTA CGCTAGTTAA CTACGTCGAC ATCGATTTAA TTAATATGCA
     7141 TCAATTGATT TAAATACTAG TGTTAACTAT TTAATTGGGG ACCCTAGAGG TCCCCTTTTT
     7201 TATTTTAAAA ATTTTTTCNA AGGGGACCTC TAGGGTCCCC AGCTGGCTAG CGAGTCATGA
     7261 GGTNACCATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT
     7321 TGAGTGAGCT GATACCAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA
     7381 CGCCAAGCTA TCAACTTTGT ATAGAAAAGT TGCATGACCA ACTTCGATAA CGTTCTCGGC
     7441 TCGATGATCC CCGGCGGGCC CCCCCTCGAG GTCGACGGTA TCGATTTAAT TAATATGCAT
     7501 CAATTGATTT AAATACTAGT GTTAACTATT TAATTGGGGA CCCTAGAGGT CCCCTTTTTT
     7561 ATTTTAAAAA TTTTTTCNAA GGGGACCTCT AGGGTCCCCA GCTGGCTAGC GAGTCATGAG
     7621 GTNACCATGG TCATTAGGAG CCGCTCTCGC ACTTCAGCTG CTTCTCCAAG CCGCAGATGA
     7681 TCAGCTCCAG GCCGAACAAG AAGGCCGGCT CGGCGCCCTG GTGGTCGAAC AGCTCGATGG
     7741 CCTGGCGCAG CAACGGCGGC ATGCTGTCGG TGGTCGGGGT CTCGCGCTCC TCCTTGGCGA
     7801 CCTGGTGCTC CTGGTCCTCC AACACGCAGC CCAGGGTGAA GTGGCCCACG GCCGACAGGG
     7861 CGTACAACGC GTTCTCCAGC GAGAAGCCCT GCTGGCACAG GAAGGCCAAC TGGTTCTCCA
     7921 GGGTCTCGTA CTGCTTCTCG GTCGGCCGGG TGCCCAGGTG CACCTTGGCG CCGTCGCGGT
     7981 GGCTCAGCAA GGCGCAGCGG AAGCTCTTGG CGTTGTTGCG CAGGAAGTCC TGCCAGCTCT
     8041 CGCCCTCCAG CGGGCAGAAG TGGGTGTGGT GGCGGTCCAA CATCTCGATG GCCAGGGCGT
     8101 CGAGCAGGGC CCGCTTGTTC TTGACGTGCC AGTACAGGGT CGGCTGCTCG ACGCCCAGCT
     8161 TCTGGGCCAG CTTGCGGGTG GTCAGGCCCT CGATGCCGAC CTCGTTCAGC AGCTCCAGGG
     8221 CGGAGTTGAT GACCTTGGAC TTGTCCAGGC GGGACATTGC GAAGTGATTC CTCCTGATGG
     8281 TACCGGATCC GTGGCAGGAG GGTCGCCTGC GTCGGCAACT CGCCCAGGCT ACACGAGAGC
     8341 CCTCGACTGC GAAAATGCCG ACCAGGCACC ACCGCACTCC AGGCTTAACC CGGTGCGACG
     8401 ATCCAATATT ACGAGATCTG ACAAGTTTGT ACAAAAAAGC AGGCTACCAG GCCTAGATCT
     8461 GGGGACCCTA GAGGTCCCCT TTTTTATTTT AAAAATTTTT TCACGTGCCA GCCGATTGGA
     8521 CTAGAAATAT TGGATCGTCG GCACCGTCAC GGCCGTGGGA GGCGGCACGA TCCGCGACGT
//
