LOCUS       pMEXC3GH_(Apr)      8895 bp    DNA     circular UNA 12-Nov-2013
DEFINITION  
FEATURES             Location/Qualifiers
     Fragment        join(7341..8887,<4011..5435,<2029..>4010,8888,8889..8895,1..>354)
                     /origin="C3GH (6'288bp linear)Fragment: from XbaI cut at
                     5^6 to BbsI cut at 6272^6273, 6'267bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     Fragment        join(<4011..8888,2029..>4010,8889..8895,1..354)
                     /origin="pMEX_C3GH dccdB (8'172bp circular)Fragment: from
                     SapI cut at 1660^1661 to SapI cut at 362^363,
                     6'874bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     promoter        156..218
                     /label="P(myc1)"
     Fragment        355..2028
                     /origin="ccdB CM (2'780bp linear)Fragment: from SapI cut
                     at 130^131 to SapI cut at 1804^1805, 1'674bpOrientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     CDS             complement(359..365)
                     /label="SapI"
     gene            complement(403..708)
                     /gene="ccdB"
                     /product="ccdB gene"
     misc_feature    complement(808..836)
                     /gene="-8.0"
     gene            complement(1050..1709)
                     /gene="cat"
                     /product="Chloramphenicol resistance gene"
     CDS             2021..2027
                     /label="SapI"
     CDS             2032..2055
                     /label="C3"
     gene            2056..2811
                     /label="GFP-His10"
     oriT            3040..3659
                     /label="pBR322 OriE"
     gene            4163..4939
                     /label="Apr"
     Fragment        5436..7340
                     /origin="OriM (1'926bp linear)Fragment: from XbaI cut at
                     5^6 to BbsI cut at 1910^1911, 1'905bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     CDS             complement(5440..7339)
                     /label="OriM"
     gene            complement(7809..8618)
                     /label="TetR(B_syn207)"
     promoter        complement(8628..8771)
                     /label="P(imyc)"
ORIGIN
        1 TCGTCGGCAC CGTCACGGCC GTGGGAGGCG GCACGATCCG CGACGTGATG ATCGGCCGCA
       61 TCCCCACGGT GCTGCGCAGT GAGCTCTACG CCATCCCGGC GTTGATCTGT GCGTTCGCAC
      121 GCACAGGCCC GGTGTGAGAA GGGTCTCTGA CGAGCGGGAG AACTCCCTAT CAGTGATAGA
      181 GTTTGTCCTC CCTATCAGTG ATAGATAGGC TCTGGGAGTA CCCGTGTGTA CGACCAGCAC
      241 GGCATACATC ATTTCGACGC CGAGAGATTC GCCGCCCGAA ATGAGCACGA TCCGCATGCA
      301 CCCAGCTTTC TTGTACAAAG TGGGCTCGAG GCATGCGAAG GAGATATACA TATGAGTTGA
      361 AGAGCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC CCCAGAACAT
      421 CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA CTTCTTCCCC
      481 GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC TTTCATCCCC
      541 GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC GTGCACTGGC
      601 CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA CATCCACAAA
      661 CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT CACCAGCCCC
      721 TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA GCCATCCCTT
      781 CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC TGCATGGTTG
      841 TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT AGCTGTCGCT
      901 GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT ACTTCGGGTA
      961 TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC GCAAATACGC ATACTGTTAT
     1021 CTGGCTTTTA GTAAGCCGGA TCCACGCGTT TACGCCCCGC CCTGCCACTC ATCGCAGTAC
     1081 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC
     1141 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA
     1201 ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC
     1261 CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG
     1321 TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG
     1381 TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA
     1441 GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAATTCCGGA
     1501 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT
     1561 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT
     1621 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG
     1681 GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAC
     1741 GGATCCTAAC TCAAAATCCA CACATTATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG
     1801 GTGCCTAATG CGGCCGCCAA TATGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT
     1861 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT
     1921 CAGCTTTTTT GTACAAACTT GTGATATCGA ATTCGCTAGC TACCTTTCTC TTCTTTTTTG
     1981 GATCTACCTT TCTCTTCTTT TTTGGGGATC CACTAGTTCT GCTCTTCTGC ATTAGAAGTT
     2041 TTGTTTCAAG GTCCACAATT CAGCAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT
     2101 CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA
     2161 GGTGATGCTA CATACGGAAA ACTCACCCTT AAATTTATTT GCACTACTGG AAAACTACCT
     2221 GTTCCTTGGC CAACACTTGT CACTACTCTG ACCTATGGTG TTCAATGCTT TTCCCGTTAT
     2281 CCGGATCACA TGAAACGGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG
     2341 GAACGCACTA TATCTTTCAA AGATGACGGG AACTACAAGA CGCGTGCTGA AGTCAAGTTT
     2401 GAAGGTGATA CCCTTGTTAA TCGTATCGAG TTAAAGGGTA TTGATTTTAA AGAAGATGGA
     2461 AACATTCTCG GACACAAACT AGAGTACAAC TATAACTCAC ACAATGTATA CATCACGGCA
     2521 GACAAACAAA AGAATGGAAT CAAAGCTAAC TTCAAAATTC GCCACAACAT TGAAGATGGT
     2581 TCCGTTCAAC TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT
     2641 TTACCAGACA ACCATTACCT GTCGACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG
     2701 CGTGACCACA TGGTCCTTCT TGAGTTTGTA ACTGCTGCTG GGATTACACA TGGCATGGAT
     2761 GAGCTCTACA AAACTAGTCA TCATCATCAC CATCATCATC ACCATCATTA ATTATACATA
     2821 GTTGCTTAAG CTGCAGGGGA CCCTAGAGGT CCCCTTTTTT ATTTTAAAAA TTTTTTATAA
     2881 CCGGTAAGAA GTCCAACACG CCGCTCCTCC CCGATGTTGC GTAGCCGCTT CAAGCTTAAG
     2941 CAACGGCCCC AGTGAGTAGC GACTTTGGAT CCGGTACCCG CAACGACTGG CCGACATGTG
     3001 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     3061 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     3121 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     3181 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     3241 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     3301 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     3361 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     3421 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     3481 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     3541 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     3601 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     3661 TTCTACGGGG TCTGACGCTC AGTGGAACTA GTGAGGTCTG CCTCGTGAAG AAGGTGTTGC
     3721 TGACTCATAC CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT
     3781 GATGAGAGCT TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT TTGCCACGGA
     3841 ACGGTCTGCG TTGTCGGGAA GATGCGTGAT CTGATCCTTC AACTCAGCAA AAGTTCGATT
     3901 TATTCAACAA AGCCGCCGTC CCGTCAAGTC AGCGTAATGC TCTGCCAGTG TTACAACCAA
     3961 TTAACCAATT CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA GTATCTAGAA
     4021 GGATCTTCAC CTAGATCCTT TTGGTTCATG TGCAGCTCCA TCAGCAAAAG GGGATGATAA
     4081 GTTTATCACC ACCGACTATT TGCAACAGTG CCGTTGATCG TGCTATGATC GACTGATGTC
     4141 ATCAGCGGTG GAGTGCAATG TCGTGCAATA CGAATGGCGA AAAGCCGAGC TCATCGGTCA
     4201 GCTTCTCAAC CTTGGGGTTA CCCCCGGCGG TGTGCTGCTG GTCCACAGCT CCTTCCGTAG
     4261 CGTCCGGCCC CTCGAAGATG GGCCACTTGG ACTGATCGAG GCCCTGCGTG CTGCGCTGGG
     4321 TCCGGGAGGG ACGCTCGTCA TGCCCTCGTG GTCAGGTCTG GACGACGAGC CGTTCGATCC
     4381 TGCCACGTCG CCCGTTACAC CGGACCTTGG AGTTGTCTCT GACACATTCT GGCGCCTGCC
     4441 AAATGTAAAG CGCAGCGCCC ATCCATTTGC CTTTGCGGCA GCGGGGCCAC AGGCAGAGCA
     4501 GATCATCTCT GATCCATTGC CCCTGCCACC TCACTCGCCT GCAAGCCCGG TCGCCCGTGT
     4561 CCATGAACTC GATGGGCAGG TACTTCTCCT CGGCGTGGGA CACGATGCCA ACACGACGCT
     4621 GCATCTTGCC GAGTTGATGG CAAAGGTTCC CTATGGGGTG CCGAGACACT GCACCATTCT
     4681 TCAGGATGGC AAGTTGGTAC GCGTCGATTA TCTCGAGAAT GACCACTGCT GTGAGCGCTT
     4741 TGCCTTGGCG GACAGGTGGC TCAAGGAaAA GAGCCTTCAG AAGGAAGGTC CAGTCGGTCA
     4801 TGCCTTTGCT CGGTTGATCC GCTCCCGCGA CATTGTGGCG ACAGCCCTGG GTCAACTGGG
     4861 CCGAGATCCG TTGATCTTCC TGCATCCGCC AGAGGCGGGA TGCGAAGAAT GCGATGCCGC
     4921 TCGCCAGTCG ATTGGCTGAG CTCATGAGCG GAGAACGAGA TGACGTTGGA GGGGCAAGGT
     4981 CGCGCTGATT GCTGGGGCAA CACGTGGAGC GGATCGGGGA TTGTCTTTCT TCAGCTCGCT
     5041 GATGATATGC TGACGCTCAA TGCCGTTTGG CCTCCGACTA ACGAAAATCC CGCATTTGGA
     5101 CGGCTGATCC ATGGTACTCC CGTTGAATAT GGCTCATAAC ACCCCTTGTA TTACTGTTTA
     5161 TGTAAGCAGA CAGTTTTATT GTTCATGATG ATATATTTTT ATCTTGTGCA ATGTAACATC
     5221 AGAGATTTTG AGACACAACG TCGCTTTGTT GCTAGCTGGC GGGAACGGCA ATGCTCCTGG
     5281 CGGCGGCGGG GCTGGTGCCC AGGTCTCCGC ACAGAGCGGC GGTGCTGGCG CTCGCGGCCA
     5341 GGCGTGGTTC TTCGCGTACT GACAAGAAAC CCCCCTCTTT AGGACTCAGT GTCCTTGGGA
     5401 GGGGGGCTTT TTGCGTTTCA GGAGGTCTTG GCCAGGCATG CCACGGATGC CACCACAAGC
     5461 ACTACAACGG AGTTCGCCAC GTACATCACC ACAACCACCG ATTCTGGCGG TGAGCTCCAC
     5521 GATATTCAGC GGAAATGGCT TGGTATCGAC CAAGATTCGT AGAACCCCGT CTCGTCTGGC
     5581 TGGTATTCAA AACGGACGCA ACGAAACACG CAACGAGACA GGCATGGCCC AAACCAGAAA
     5641 ACTAGCGTCT ACCAGGACTT TTACCTGTCC GACCCGTTGC AACGGAACCC CCCACGGAAC
     5701 CCCCGCGACA CCCGCTCCCC AATTGCGTTA GAACAGCGGT GGATTGTCGG CTTCGTTGTG
     5761 GGCCTTTTGA GCCGCTTCCT GTTCTGCCGC ACGCTCTTTC CTCGCCCGAT AGCCGAGTCG
     5821 CTTAACGGTG TCCAGATGCA GCCCGAAATG TTTGGCCGTT TGCGGCCAAG AGTGGCCCTC
     5881 GTCGTCGTGA TAGGCGCGGA TGCGTTCGCG GCGTGCAGCC TGCTCGGCGA ACCACTCGCT
     5941 GCGTTCCTGC GCCACGAGCC GGACGACGTG GCGTTCGGAT AGTCCGGTGA TTCGAGCGCC
     6001 TTCGGCGGCG GTCACGCGCC GCTTTTTGCG GACAGTCGGC TGCCAGTTGT AGCCGTCGCT
     6061 GTAGCCGTCG CTGTAGCCGT CGCTCATAGC AATGCCTCCA TGCATGGCTG ACGCGGACTT
     6121 TGCGCGCCGC GCAACTGTGC TCGCCGCCGT GCGCGCTGCT GCGCCCTTCC GCGAGATGGC
     6181 CGCATGGCGC GCACTGAGTG TGGCCTCGTA GACCACGATC CCGTCCGCCC AAATGCGCGA
     6241 CTTGGTTGTG ATCCAACGCC AAATGCTGTT GGCGATGGCG CGGACCTCGC TGTCCGGTAG
     6301 CGGTCCGGGA CACACGTCGT TGCACGGAAA TTCGGCGTTT CGCGCGTGGC ACTCGGCATA
     6361 GATCGCGCGG CCGAGTCCGT CCACGTTCCG GGTCGGCAGG TAGATCCGCA TGAGGGCGGG
     6421 AAGATAGGCC CACAACCTGA CGGAATCGAA CAGTGCGCAA TTCCGCCCTA GCGGCGTCGG
     6481 AGCCGCTTTG TACGTGGTCT GCTGACGCCA GCGCGGCGGT GGCATGTTCG CGCCGAGCTC
     6541 GGCCTCGATG TGGCTGAGTG TGTAGAGATC GGAGTGGAGC CATTCCGTTT CCCAGGCGAT
     6601 GTGGCCGGGG TTTTTGGTCA TGAGGCCTGA GTAACTGCGG TCGCCATCGA CGGCGCGCCG
     6661 AAGGCCTTCG GCGCACGCCG CCATGTATGC GAGCGGCTTA CGCCGCGCGT ATTCGGTGCG
     6721 TGGAACAGGG GCGTTGAGTG CCCACACTGC GTGTGCGTGG CCGTTGGCGC GATTGCCCAC
     6781 GATCGCGTTG GGCAGCGGAT GGGACCCCCG GGCGCTGAGC GCTCGGAGCG CTGCGTCTGG
     6841 ATGGTCTACG TCCACGACCA GCAGGTTTGC CAGCGCTGTT GGGTTCGCCT CGATGTACCG
     6901 GCGGCCTAGG GCCGACGCGC GGCTTTGGCG GTAGATCCCC TCGAGCAGAT CGTCGCTTGC
     6961 CAGGGACCAG TACGGCAGCC AGAGCTGCTC AAATTCGTCG GCGACGTGGC TCACGCTTGA
     7021 TAATAAACCA CGATTAATCA CCGGTGTATG GTCCGACACG AGCTCCAAGT CAGATATTTC
     7081 GCTGAGGGGC CACCCCACAA CTGCACACTC CCCCGCTCTC CCGTCGAGCC CTGATGATGA
     7141 AACACCAGCG ACAGCCGAGC ACCCCCAACC ACCTGTACCA ACCAGGAGGA ACACATGCGT
     7201 CGTTTCGAGG ACGTTTCCGG GCCGCTAAGA GCCGCTGTGG CGGCCGTACA CGCCGCCTTA
     7261 GACCCGTTAG ACCCCCTGCC GCCTGAATGC GCGGGTACGA GCCACACAGC ACCCGAACTT
     7321 ACGGAGCTGG TGGGCTCACT CTAGATCACG GCTCTAGCTG ATCACCGCGG CCATGATGGC
     7381 CGGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT CGTTTTATTT
     7441 GATGCCTGGC AGTCGATCGT ACGCTAGTTA ACTACGTCGA CATCGATTTA ATTAATATGC
     7501 ATCAATTGAT TTAAATACTA GTGTTAACTA TTTAATTGGG GACCCTAGAG GTCCCCTTTT
     7561 TTATTTTAAA AATTTTTTCN AAGGGGACCT CTAGGGTCCC CAGCTGGCTA GCGAGTCATG
     7621 AGGTNACCAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT
     7681 TTGAGTGAGC TGATACCAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT
     7741 ACGCCAAGCT ATCAACTTTG TATAGAAAAG TTGCATGACC AACTTCGATA ACGTTCTCGG
     7801 CTCGATGATC CCCGGCGGGC CCCCCCTCGA GGTCGACGGT ATCGATTTAA TTAATATGCA
     7861 TCAATTGATT TAAATACTAG TGTTAACTAT TTAATTGGGG ACCCTAGAGG TCCCCTTTTT
     7921 TATTTTAAAA ATTTTTTCNA AGGGGACCTC TAGGGTCCCC AGCTGGCTAG CGAGTCATGA
     7981 GGTNACCATG GTCATTAGGA GCCGCTCTCG CACTTCAGCT GCTTCTCCAA GCCGCAGATG
     8041 ATCAGCTCCA GGCCGAACAA GAAGGCCGGC TCGGCGCCCT GGTGGTCGAA CAGCTCGATG
     8101 GCCTGGCGCA GCAACGGCGG CATGCTGTCG GTGGTCGGGG TCTCGCGCTC CTCCTTGGCG
     8161 ACCTGGTGCT CCTGGTCCTC CAACACGCAG CCCAGGGTGA AGTGGCCCAC GGCCGACAGG
     8221 GCGTACAACG CGTTCTCCAG CGAGAAGCCC TGCTGGCACA GGAAGGCCAA CTGGTTCTCC
     8281 AGGGTCTCGT ACTGCTTCTC GGTCGGCCGG GTGCCCAGGT GCACCTTGGC GCCGTCGCGG
     8341 TGGCTCAGCA AGGCGCAGCG GAAGCTCTTG GCGTTGTTGC GCAGGAAGTC CTGCCAGCTC
     8401 TCGCCCTCCA GCGGGCAGAA GTGGGTGTGG TGGCGGTCCA ACATCTCGAT GGCCAGGGCG
     8461 TCGAGCAGGG CCCGCTTGTT CTTGACGTGC CAGTACAGGG TCGGCTGCTC GACGCCCAGC
     8521 TTCTGGGCCA GCTTGCGGGT GGTCAGGCCC TCGATGCCGA CCTCGTTCAG CAGCTCCAGG
     8581 GCGGAGTTGA TGACCTTGGA CTTGTCCAGG CGGGACATTG CGAAGTGATT CCTCCTGATG
     8641 GTACCGGATC CGTGGCAGGA GGGTCGCCTG CGTCGGCAAC TCGCCCAGGC TACACGAGAG
     8701 CCCTCGACTG CGAAAATGCC GACCAGGCAC CACCGCACTC CAGGCTTAAC CCGGTGCGAC
     8761 GATCCAATAT TACGAGATCT GACAAGTTTG TACAAAAAAG CAGGCTACCA GGCCTAGATC
     8821 TGGGGACCCT AGAGGTCCCC TTTTTTATTT TAAAAATTTT TTCACGTGCC AGCCGATTGG
     8881 ACTAGAAATA TTGGA
//
