LOCUS       Exported                4242 bp ds-DNA     circular SYN 28-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pKOIgalK (Kan)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(123..938)
                     /label=KanR
     CDS             complement(1257..1263)
                     /label=SapI
     CDS             1268..1274
                     /label=SapI
     oriT            1481..2069
                     /direction=RIGHT
                     /label=pUC OriE
     misc_feature    2120..4144
                     /label=Fragment
     gene            2214..4117
                     /gene="SacB"
                     /label=SacB
     promoter        2416..2659
                     /label=SacB promoter
     misc_feature    join(4145..1004,1078..2119,1005..1077)
                     /label=Fragment
ORIGIN
        1 gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag
       61 ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct
      121 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
      181 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
      241 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
      301 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
      361 actgaatccg gtgagaatgg caaaagatta tgcatttctt tccagacttg ttcaacaggc
      421 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
      481 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
      541 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
      601 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
      661 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
      721 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
      781 aacaactctg gcgcatgggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg
      841 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
      901 ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
      961 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     1021 cagagatttt gagacacaac gtggctttcc cccccccccg acgtcaggtg gctagaatcc
     1081 cattcgccat tcaggctgcg caactgttgg gaagggcgtt tcggtgcggg cctcttcgct
     1141 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     1201 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgacgtaa taagttgaag
     1261 agcatctgct cttcagcatc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1321 cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact
     1381 gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc
     1441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1921 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1981 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2041 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2101 acgctcagtg gaacgaaggc gcgcctgagt gagctgatac cgctcgccgc agccgaacga
     2161 ccgagcgcag cgagtcagtg agcgaggaag cggaagatcg ggcccagccg gcccacatat
     2221 acctgccgtt cactattatt tagtgaaatg agatattatg atattttctg aattgtgatt
     2281 aaaaaggcaa ctttatgccc atgcaacaga aactataaaa aatacagaga atgaaaagaa
     2341 acagatagat tttttagttc tttaggcccg tagtctgcaa atccttttat gattttctat
     2401 caaacaaaag aggaaaatag accagttgca atccaaacga gagtctaata gaatgaggtc
     2461 gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc aagacctaaa atgtgtaaag
     2521 ggcaaagtgt atactttggc gtcacccctt acatatttta ggtctttttt tattgtgcgt
     2581 aactaacttg ccatcttcaa acaggagggc tggaagaagc agaccgctaa cacagtacat
     2641 aaaaaaggag acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac
     2701 ctttactacc gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca
     2761 aaagccatat aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat
     2821 ccctgaacag caaaaaaatg aaaaatataa agttcctgag ttcgattcgt ccacaattaa
     2881 aaatatctct tctgcaaaag gcctggacgt ttgggacagc tggccattac aaaacactga
     2941 cggcactgtc gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa
     3001 aaatgcggat gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga
     3061 cagctggaaa aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc
     3121 tatcctaaaa gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa
     3181 aatccgttta ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac
     3241 tgcacaagtt aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggatta
     3301 taaatcaatc tttgacggtg acggaaaaac gtatcaaaat gtacagcagt tcatcgatga
     3361 aggcaactac agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa
     3421 aggccacaaa tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga
     3481 agaatcttta tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag
     3541 tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg
     3601 tatgattgag ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc
     3661 taacacagta acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta
     3721 cctgttcact gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat
     3781 ttacatgctt ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac
     3841 tggccttgtg ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt
     3901 cgctgtacct caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg
     3961 attctacgca gacaaacaat caacgtttgc gcctagcttc ctgctgaaca tcaaaggcaa
     4021 gaaaacatct gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata
     4081 aaaacgcaaa agaaaatgcc gatatcctat tggcattgcg gccgcggtct cggcggtggg
     4141 tcgtctagag aaagtgaggg agccacggtt ggtgagagct ttgttgtagg tggaccagtt
     4201 ggtgattttg aacttttgct ttgccacgga acggtctgcg tt
//
