LOCUS       pFLAG_attP      5681 bp    DNA     circular UNA 19-Feb-2016
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        95..157
                     /label="pTET"
     CDS             complement(298..304)
                     /label="sapI"
     gene            complement(342..647)
                     /label="ccdB"
     gene            complement(989..1648)
                     /label="cat"
     CDS             1960..1966
                     /label="sapI"
     CDS             1971..2036
                     /label="FLAG"
     CDS             2211..2830
                     /label="oriE"
     gene            complement(3415..4191)
                     /label="APR"
     CDS             complement(4529..5031)
                     /label="attP"
ORIGIN
        1 CCCCACGGTG CTGCGCAGTG AGCTCTACGC CATCCCGGCG TTGATCTGTG CGTTCGCACG
       61 CACAGGCCCG GTGTGAGAAG GGTCTCTGAC GAGCGGGAGA ACTCCCTATC AGTGATAGAG
      121 TTTGTCCTCC CTATCAGTGA TAGATAGGCT CTGGGAGTAC CCGTGTGTAC GACCAGCACG
      181 GCATACATCA TTTCGACGCC GAGAGATTCG CCGCCCGAAA TGAGCACGAT CCGCATGCAC
      241 CCAGCTTTCT TGTACAAAGT GGGCTCGAGG CATGCGAAGG AGATATACAT ATGAGTTGAA
      301 GAGCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC
      361 AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG
      421 ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG
      481 ATATGCACCA CCGGGTAAAG TTCACGGGAG ACTTTATCTG ACAGCAGACG TGCACTGGCC
      541 AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT CACTCTGTAC ATCCACAAAC
      601 AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA ACTGCATTTC ACCAGCCCCT
      661 GTTCTCGTCA GCAAAAGAGC CGTTCATTTC AATAAACCGG GCGACCTCAG CCATCCCTTC
      721 CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG GCTTCATTCT GCATGGTTGT
      781 GCTTACCAGA CCGGAGATAT TGACATCATA TATGCCTTGA GCAACTGATA GCTGTCGCTG
      841 TCAACTGTCA CTGTAATACG CTGCTTCATA GCATACCTCT TTTTGACATA CTTCGGGTAT
      901 ACATATCAGT ATATATTCTT ATACCGCAAA AATCAGCGCG CAAATACGCA TACTGTTATC
      961 TGGCTTTTAG TAAGCCGGAT CCACGCGTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT
     1021 GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT
     1081 GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA
     1141 CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC
     1201 AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT
     1261 TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT
     1321 GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG
     1381 GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG AATTCCGGAT
     1441 GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT
     1501 TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT
     1561 GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG
     1621 TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGACG
     1681 GATCCTAACT CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
     1741 TGCCTAATGC GGCCGCCAAT ATGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG
     1801 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC
     1861 AGCTTTTTTG TACAAACTTG TGATATCGAA TTCGCTAGCT ACCTTTCTCT TCTTTTTTGG
     1921 ATCTACCTTT CTCTTCTTTT TTGGGGATCC ACTAGTTCTG CTCTTCTGCA GACTACAAAG
     1981 ACCATGACGG TGATTATAAA GATCATGATA TCGATTACAA GGATGACGAT GACAAGTAGT
     2041 GAGCATTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
     2101 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
     2161 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     2221 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     2281 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     2341 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     2401 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     2461 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     2521 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     2581 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     2641 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     2701 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     2761 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     2821 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2881 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2941 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     3001 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     3061 GGACGTGGCC GACCAGCCCG TCATCGTCAA CGCCTGACCG CGGTGCGGAC AGGCCGTGTC
     3121 GCGACCGGCC GTGCGGAATT AAGCCGGCCC GTACCCTGTG AATAGAGGTC CGCTGTGACA
     3181 CAAGAATCCC TGTTACTTCT CGACCGTATT GATTCGGATG ATTCCTACGC GAGCCTGTAC
     3241 CATGGATCAG CCGTCCAAAT GCGGGATTTT CGTTAGTCGG AGGCCAAACG GCATTGAGCG
     3301 TCAGCATATC ATCAGCGAGC TGAAGAAAGA CAATCCCCGA TCCGCTCCAC GTGTTGCCCC
     3361 AGCAATCAGC GCGACCTTGC CCCTCCAACG TCATCTCGTT CTCCGCTCAT GAGCTCAGCC
     3421 AATCGACTGG CGAGCGGCAT CGCATTCTTC GCATCCCGCC TCTGGCGGAT GCAGGAAGAT
     3481 CAACGGATCT CGGCCCAGTT GACCCAGGGC TGTCGCCACA ATGTCGCGGG AGCGGATCAA
     3541 CCGAGCAAAG GCATGACCGA CTGGACCTTC CTTCTGAAGG CTCTTtTCCT TGAGCCACCT
     3601 GTCCGCCAAG GCAAAGCGCT CACAGCAGTG GTCATTCTCG AGATAATCGA CGCGTACCAA
     3661 CTTGCCATCC TGAAGAATGG TGCAGTGTCT CGGCACCCCA TAGGGAACCT TTGCCATCAA
     3721 CTCGGCAAGA TGCAGCGTCG TGTTGGCATC GTGTCCCACG CCGAGGAGAA GTACCTGCCC
     3781 ATCGAGTTCA TGGACACGGG CGACCGGGCT TGCAGGCGAG TGAGGTGGCA GGGGCAATGG
     3841 ATCAGAGATG ATCTGCTCTG CCTGTGGCCC CGCTGCCGCA AAGGCAAATG GATGGGCGCT
     3901 GCGCTTTACA TTTGGCAGGC GCCAGAATGT GTCAGAGACA ACTCCAAGGT CCGGTGTAAC
     3961 GGGCGACGTG GCAGGATCGA ACGGCTCGTC GTCCAGACCT GACCACGAGG GCATGACGAG
     4021 CGTCCCTCCC GGACCCAGCG CAGCACGCAG GGCCTCGATC AGTCCAAGTG GCCCATCTTC
     4081 GAGGGGCCGG ACGCTACGGA AGGAGCTGTG GACCAGCAGC ACACCGCCGG GGGTAACCCC
     4141 AAGGTTGAGA AGCTGACCGA TGAGCTCGGC TTTTCGCCAT TCGTATTGCA CGACATTGCA
     4201 CTCCACCGCT GATGACATCA GTCGATCATA GCACGATCAA CGGCACTGTT GCAAATAGTC
     4261 GGTGGTGATA AACTTATCAT CCCCTTTTGC TGATGGAGCT GCACATGAAC CAAAAGGATC
     4321 TAGGTGAAGA TCCTTCTAGA TACATCAACG AGGACAGTCG CACGACGAAG TTCTTCTGGA
     4381 TCGCGCCCGT GCTGGAAGCA CTCAACCTCG AAGCGTGTGG TTGCGGAGCC ATCTAGCAAC
     4441 CACACGAGGA TCTTGTTGTC GTAGGTCTGC AGCGCGTAGT ACCTCACACC GTCCTGCGGG
     4501 TTGACGTATG AGGCTTGGAT CCTCCCGCTG CGCTGAGTCT TCAGCGATCC CCATCCGCGA
     4561 CGTGCCAACT AGGTCTCCTC TCGTCGTGAA CAAGGCTACC GGGTTGCAAC TCCTGTGCAA
     4621 CTCTCAGGCT TCAACGCGCT TCTACGACCT GCAATTTCTT TCCACTTAGA GGATGCAGCC
     4681 GAGAGGGGGT AAAAACCTAT CTTGACCGGC CCATATATGT GGTCGGCAGA CACCCATTCT
     4741 TCCAAACTAG CTACGCGGGT TCGATTCCCG TCGCCCGCTC CGCTGGTCAG AGGGTGTTTT
     4801 CGCCCTCTGG CCATTTTTCT TTCCAGGGGT CTGCAACTCT TGTGCGACTC TTCTGACCTG
     4861 GGCATACGCG GTTGCAACGC ATCCCTGATC TGGCTACTTT CGATGCTGAC AAACGAATAG
     4921 AGCCCCCCGC CTGCGCGAAC AGACGAGGGG CATTCACACC AGATTGGAGC TGGTGCAGTG
     4981 AAGAGAATAG ACCGGGACAA GGTTGCACCG GGAGTTGCAG CGGTCGGAAC CCTCGCCGTC
     5041 GGCGGGCTGG CGTTCGCCCT GTCGTTCACG GCTCTAGCTG ATCACCGCGG CCATGATGGC
     5101 CGGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT CGTTTTATTT
     5161 GATGCCTGGC AGTCGATCGT ACGCTAGTTA ACTACGTCGA CATCGATTTA ATTAATATGC
     5221 ATCAATTGAT TTAAATACTA GTGTTAACTA TTTAATTGGG GACCCTAGAG GTCCCCTTTT
     5281 TTATTTTAAA AATTTTTTCN AAGGGGACCT CTAGGGTCCC CAGCTGGCTA GCGAGTCATG
     5341 AGGTNACCAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT
     5401 TTGAGTGAGC TGATACCAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT
     5461 ACGCCAAGCT ATCAACTTTG TATAGAAAAG TTGCATGACC AACTTCGATA ACGTTCTCGG
     5521 CTCGATGATC CACCAGGCCT AGATCTGGGG ACCCTAGAGG TCCCCTTTTT TATTTTAAAA
     5581 ATTTTTTCAC GTGCCAGCCG ATTGGACTAG AAATATTGGA TCGTCGGCAC CGTCACGGCC
     5641 GTGGGAGGCG GCACGATCCG CGACGTGATG ATCGGCCGCA T
//
