LOCUS       pMA_Int      3716 bp    DNA     circular UNA 30-Sep-2013
DEFINITION  
FEATURES             Location/Qualifiers
     Fragment        join(1784..3716,1..344)
                     /origin="pMA_SAP (4) [#7] (2'300bp circular)Fragment:
                     from SapI cut at 347^348 to SapI cut at 324^325,
                     2'277bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     Fragment        345..1783
                     /origin="Integrase FX (1'470bp linear)Fragment: from SapI
                     cut at 14^15 to SapI cut at 1453^1454,
                     1'439bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     gene            complement(520..1518)
                     /label="Integrase"
     CDS             complement(1596..>1783)
                     /label="ΔattP"
     Site            1672..1681
                     /site_type="binding site"
                     /label="P1"
     Site            1683..1692
                     /site_type="binding site"
                     /label="P2"
     Site            complement(1735..1744)
                     /site_type="binding site"
                     /label="P3"
     oriT            1989..2577
                     /label="pUC OriE"
     gene            complement(2748..3608)
                     /label="AmpR"
ORIGIN
        1 ATTTCCCCGA AAAGTGCCAC CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA
       61 AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
      121 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC
      181 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC
      241 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC
      301 AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGACGT AATAAGTTTG TTGCTAGCTG
      361 GCGGGAACGG CAATGCTCCT GGCGGCGGCG GGGCTGGTGC CCAGGTCTCC GCACAGAGCG
      421 GCGGTGCTGG CGCTCGCGGC CAGGCGTGGT TCTTCGCGTA CTGACAAGAA ACCCCCCTCT
      481 TTAGGACTCA GTGTCCTTGG GAGGGGGGCT TTTTGCGTTT CAGGAGGTCT TGGCCAGCTT
      541 GGACATCGCC TCAGCGATAG CCTCGTCGCG GGCCTCAGAC GCCATCTGGT ACTTCATCGC
      601 CATCCTAGGA GTCGTGTGAC CGAGACGGGC CATCAGCTCC TTGGTCGTCG CACCTGCCTG
      661 AGCGGCGAAC GTAGCGCCGA CAGCGCGGAG GTCGTGGATG CGGAGTTCCG GCCGACCGAT
      721 CTTGGCGTAG CCACGCTTCA GCGACTTGGT GAACGCGGAC TTCGACAGCC GGTTGCCCTG
      781 CGTCGTGGTC ACCAGGAATG CCTCGGGGCC CTTGTTCATC TTCGTACGGT CCTTCATGTG
      841 CGCTCGGATC ATCTCCGCGA CGTGAGGCGG AACCGTCACA GGACGCTTCG ACCGGACGGT
      901 CTTGGCGTTG CCAACGACGA TCTTGTTCCC CACGCGGGAA GCGCCACGGC GCACCCGGAG
      961 CTTCATCGTC ATGCCGTCGT CCACGATGTC CTTGCGGCGA AGCTCGATCA GCTCTCCGAA
     1021 CCGGAGGCTC GTCCACGCCA GGATGTATGC CGCGATCCGG TAGTGCTCGA AGATCTCAGC
     1081 GGCGACGATG TCCAGCTCCT CAGGCGTCAG CGCCTCTACG TCGCGCTCAT CGGCTGCCTT
     1141 CTGCTCGATC CGGCACGGGT TCTCTGCGAT CAGCTTGTCC TCGACCGCTG TGTTCATCAC
     1201 CGCCCGGAGG ACGTTGTAGG CATGCCGGCG GGCAGTCGGG TGCTTCCTAC CCATCCCGGC
     1261 CCACCACGCA CGCACCAGAG CTGGCGTCAT CTCTGTGACC GCCACTTCAC CTAGCACCGG
     1321 GTAGATGCGG CGCTCCGCGT GCCCGCTGTA CAGATCCCTG GTGCCGTCTG CGAGGTCGCG
     1381 CTCCACGAGC CACTTCCGGG TGTACTCCTC CAGCGTGATG GCGCTGGCGG CTGCCTTCTT
     1441 CGCCCGGTCC TGTGGAGGGG TCCAGGTCTC CATCTCGATG AGCCGCTTCT CGCCCGCGAG
     1501 CCAGGCTTCG GCGTCCATCT TGTTGTCGTA GGTCTGCAGC GCGTAGTACC TCACACCGTC
     1561 CTGCGGGTTG ACGTATGAGG CTTGGATCCT CCCGCTGCGC TGAGTCTTCA GCGATCCCCA
     1621 TCCGCGACGT GCCAACTAGG TCTCCTCTCG TCGTGAACAA GGCTACCGGG TTGCAACTCC
     1681 TGTGCAACTC TCAGGCTTCA ACGCGCTTCT ACGACCTGCA ATTTCTTTCC ACTTAGAGGA
     1741 TGCAGCCGAG AGGGGGTAAA AACCTATCTT GACCGGCCCA TATgcaTCAC TGCCCGCTTT
     1801 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAACATGGT CATAGCTGTT TCCTTGCGTA
     1861 TTGGGCGCTC TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGGTAAAGCC
     1921 TGGGGTGCCT AATGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     1981 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     2041 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     2101 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     2161 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     2221 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     2281 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     2341 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     2401 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     2461 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     2521 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     2581 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2641 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2701 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2761 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2821 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2881 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2941 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     3001 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     3061 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     3121 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     3181 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3241 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3301 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3361 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3421 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3481 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3541 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3601 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3661 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCAC
//
