LOCUS Exported 6991 bp ds-DNA circular SYN 28-MAR-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBXPHM3FX_[#190] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6991) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 28, 2018 from SnapGene 4.1.7 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6991 /organism="synthetic DNA construct" /mol_type="other DNA" gene 319..384 /label=pelB CDS 385..423 /label=linker gene 424..453 /label=10xHis gene 454..1566 /label=MBP CDS 1567..1590 /label=3C CDS complement(1595..1601) /label=SapI gene complement(1639..1944) /label=ccdB gene complement(2286..2945) /label=cat CDS 3257..3263 /label=SapI gene 3878..4738 /label=AmpR CDS 4893..5512 /label=pBR322 gene complement(6087..25) /label=AraC ORIGIN 1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggcact 61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 301 taacaggagg aattaaccat gaaatacctg ctgccgaccg ctgctgctgg tctgctgctc 361 ctcgctgccc agccggcgat ggccatggat atcggaatta attcggatcc gaattcgagc 421 tcacatcacc atcaccatca ccatcaccat caccccatga aaatcgaaga aggtaaactg 481 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc 541 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc 601 ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt 661 ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac 721 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg 781 atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa 841 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg 901 atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat 961 gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc 1021 gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac 1081 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac 1141 ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg 1201 ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac 1261 gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat 1321 gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac 1381 gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc ccagaaaggt 1441 gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg 1501 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactccg 1561 ggtagcctgg aagttctgtt ccaggggccc agttgaagag cgacctgcag actggctgtg 1621 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc 1681 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc 1741 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc 1801 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc 1861 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt 1921 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt 1981 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt 2041 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga 2101 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg 2161 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata 2221 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca 2281 cgcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 2341 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc 2401 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 2461 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 2521 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 2581 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 2641 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 2701 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga 2761 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 2821 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 2881 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 2941 tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa aatccacaca 3001 ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg 3061 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta 3121 atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac aaacttgtga 3181 tatcgaattc gctagctacc tttctcttct tttttggatc tacctttctc ttcttttttg 3241 gggatccact agttcagctc ttctgcataa ctagaacaaa aactcatctc agaagaggat 3301 ctgaatagcg ccgtcgacca tcatcatcat catcattgag tttaaacggt ctccagcttg 3361 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa 3421 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca 3481 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga 3541 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3601 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg 3661 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact 3721 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa 3781 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 3841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 3901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 3961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 4021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 4081 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 4141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 4201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 4261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 4321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 4381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 4441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 4501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 4561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 4621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 4681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 4741 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 4801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 4861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5041 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 5581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 5641 gaccgagcgc agcgagtcag tgagcgagga agcggaagtg cgcctgatgc ggtattttct 5701 ccttacgcat ctggtatgtc aaaaaaccct atctagaaaa ccctcgatcg ttcgtcagtg 5761 cgatcgtacg atcgagcggc cgttagtgcg cacgacctca cgacagtgcg agcggccgtt 5821 cggccgtgcg cgcgttctag cgtccgacct cacggccgat ctatagtgct atcgccctac 5881 ctcccgcccg tgcgatcgcc ctatagtgct atcgccctac ctcccgcccg tgcgatcgcc 5941 agtgcatgct cccgatcgta cgcccgttct cacggccgca cgccccatcg tccgacctag 6001 ctcacaatcg ttcgtgctca cgaccggccg tgcgccctag ccatcgccct acctcccgcc 6061 cgtgcgatcg cccccgcgac cgtactcccg ccaatgtgag ctgacctgaa cgagaacgtg 6121 cgataaagaa aaaaaactga ctagaaaaca tccgatcgtt cgtcagtgcg atcgtacgat 6181 cgagcggccg ttagtgcgca cgacctcacg acagtgcgag cggccgttcg gccgtgcgcg 6241 cgttctagcg tccgacctca cggccgatct atagtgctat cgccctacct cccgcccgtg 6301 cgatcgccct atagtgctat cgccctacct cccgcccgtg cgatcgccag tgccatcgtc 6361 cgacctagct cacaatcgtt cgtgctcacg accggccgtg cgccctagcc atcgccctac 6421 ctcccgcccg tgcgatcgcc cccgcgaccg tactcccgcc ggacccagaa actcgacaat 6481 atttcagcag tcaaagaaaa acgcccggaa aaacaacgcg cgcccgacct cactccctag 6541 cgccctatcc ttcggcctat cacccgaccg ctcgccctag cagcaaaaaa tgcgcgcgcc 6601 cgacctcact ccctagcgcc ctatccttcg ttcgagcggg cagccgcacc ggaaaaaaga 6661 cggcctatca tacggccgtg cgcccgacct agcagcaaaa aacgcgtacg cccgtgcgct 6721 ctcacggacc ggaaaaaatt ctatctgccg tccgagcgtt cgtactatcc ttcggcctat 6781 cacccgaccg ctcgccctag cagcaaaaaa tcctatctgc cgtccgagcg ttcgtactat 6841 ccttcgttcg agcgggcagc cgcacagcaa aaaatactca ctagcgaccg atcggtctat 6901 ccttcgttcg agcgggcagc cgcacataaa aaaagccgac cgatcgatcg ccctatctat 6961 cggccgttcg tgctcaaaaa acagcatacg g //