LOCUS       Exported                6991 bp ds-DNA     circular SYN 28-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBXPHM3FX_[#190]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            319..384
                     /label=pelB
     CDS             385..423
                     /label=linker
     gene            424..453
                     /label=10xHis
     gene            454..1566
                     /label=MBP
     CDS             1567..1590
                     /label=3C
     CDS             complement(1595..1601)
                     /label=SapI
     gene            complement(1639..1944)
                     /label=ccdB
     gene            complement(2286..2945)
                     /label=cat
     CDS             3257..3263
                     /label=SapI
     gene            3878..4738
                     /label=AmpR
     CDS             4893..5512
                     /label=pBR322
     gene            complement(6087..25)
                     /label=AraC
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggcact
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gaaatacctg ctgccgaccg ctgctgctgg tctgctgctc
      361 ctcgctgccc agccggcgat ggccatggat atcggaatta attcggatcc gaattcgagc
      421 tcacatcacc atcaccatca ccatcaccat caccccatga aaatcgaaga aggtaaactg
      481 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
      541 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
      601 ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt
      661 ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
      721 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg
      781 atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa
      841 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
      901 atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat
      961 gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc
     1021 gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac
     1081 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac
     1141 ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg
     1201 ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac
     1261 gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat
     1321 gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac
     1381 gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc ccagaaaggt
     1441 gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
     1501 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactccg
     1561 ggtagcctgg aagttctgtt ccaggggccc agttgaagag cgacctgcag actggctgtg
     1621 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
     1681 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
     1741 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
     1801 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
     1861 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
     1921 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
     1981 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
     2041 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
     2101 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
     2161 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
     2221 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca
     2281 cgcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     2341 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2401 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2461 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2521 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2581 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2641 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2701 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     2761 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2821 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2881 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2941 tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa aatccacaca
     3001 ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg
     3061 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     3121 atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac aaacttgtga
     3181 tatcgaattc gctagctacc tttctcttct tttttggatc tacctttctc ttcttttttg
     3241 gggatccact agttcagctc ttctgcataa ctagaacaaa aactcatctc agaagaggat
     3301 ctgaatagcg ccgtcgacca tcatcatcat catcattgag tttaaacggt ctccagcttg
     3361 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
     3421 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     3481 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     3541 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3601 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     3661 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3721 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     3781 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4081 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     4141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4741 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5041 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5641 gaccgagcgc agcgagtcag tgagcgagga agcggaagtg cgcctgatgc ggtattttct
     5701 ccttacgcat ctggtatgtc aaaaaaccct atctagaaaa ccctcgatcg ttcgtcagtg
     5761 cgatcgtacg atcgagcggc cgttagtgcg cacgacctca cgacagtgcg agcggccgtt
     5821 cggccgtgcg cgcgttctag cgtccgacct cacggccgat ctatagtgct atcgccctac
     5881 ctcccgcccg tgcgatcgcc ctatagtgct atcgccctac ctcccgcccg tgcgatcgcc
     5941 agtgcatgct cccgatcgta cgcccgttct cacggccgca cgccccatcg tccgacctag
     6001 ctcacaatcg ttcgtgctca cgaccggccg tgcgccctag ccatcgccct acctcccgcc
     6061 cgtgcgatcg cccccgcgac cgtactcccg ccaatgtgag ctgacctgaa cgagaacgtg
     6121 cgataaagaa aaaaaactga ctagaaaaca tccgatcgtt cgtcagtgcg atcgtacgat
     6181 cgagcggccg ttagtgcgca cgacctcacg acagtgcgag cggccgttcg gccgtgcgcg
     6241 cgttctagcg tccgacctca cggccgatct atagtgctat cgccctacct cccgcccgtg
     6301 cgatcgccct atagtgctat cgccctacct cccgcccgtg cgatcgccag tgccatcgtc
     6361 cgacctagct cacaatcgtt cgtgctcacg accggccgtg cgccctagcc atcgccctac
     6421 ctcccgcccg tgcgatcgcc cccgcgaccg tactcccgcc ggacccagaa actcgacaat
     6481 atttcagcag tcaaagaaaa acgcccggaa aaacaacgcg cgcccgacct cactccctag
     6541 cgccctatcc ttcggcctat cacccgaccg ctcgccctag cagcaaaaaa tgcgcgcgcc
     6601 cgacctcact ccctagcgcc ctatccttcg ttcgagcggg cagccgcacc ggaaaaaaga
     6661 cggcctatca tacggccgtg cgcccgacct agcagcaaaa aacgcgtacg cccgtgcgct
     6721 ctcacggacc ggaaaaaatt ctatctgccg tccgagcgtt cgtactatcc ttcggcctat
     6781 cacccgaccg ctcgccctag cagcaaaaaa tcctatctgc cgtccgagcg ttcgtactat
     6841 ccttcgttcg agcgggcagc cgcacagcaa aaaatactca ctagcgaccg atcggtctat
     6901 ccttcgttcg agcgggcagc cgcacataaa aaaagccgac cgatcgatcg ccctatctat
     6961 cggccgttcg tgctcaaaaa acagcatacg g
//
