LOCUS       Exported                5680 bp ds-DNA     circular SYN 28-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBXPHM3FX_scaffold_loop_library
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            319..384
                     /label=pelB
     CDS             385..423
                     /label=linker
     gene            424..453
                     /label=10xHis
     gene            454..1566
                     /label=MBP
     CDS             1567..1590
                     /label=3C
     CDS             1591..1956
                     /label=scaffold Loop Library
     gene            2567..3427
                     /label=AmpR
     CDS             3582..4201
                     /label=pBR322
     gene            complement(4776..25)
                     /label=AraC
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggcact
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gaaatacctg ctgccgaccg ctgctgctgg tctgctgctc
      361 ctcgctgccc agccggcgat ggccatggat atcggaatta attcggatcc gaattcgagc
      421 tcacatcacc atcaccatca ccatcaccat caccccatga aaatcgaaga aggtaaactg
      481 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
      541 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
      601 ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt
      661 ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
      721 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg
      781 atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa
      841 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
      901 atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat
      961 gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc
     1021 gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac
     1081 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac
     1141 ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg
     1201 ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac
     1261 gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat
     1321 gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac
     1381 gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc ccagaaaggt
     1441 gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
     1501 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactccg
     1561 ggtagcctgg aagttctgtt ccaggggccc agtcaggttc agctggttga gagcggtggt
     1621 ggcctggtcc aagctggcgg ttcgctgcgt ctgagctgcg ccgcaagcgg tttcccggtg
     1681 agcagcagca cgatgacctg gtatcgtcag gcaccgggca aagaacgtga gtgggtcgcg
     1741 gcgatttcca gctctggtag caccacgacc tacgcagatt ctgttaaggg ccgctttacc
     1801 atcagccgcg acaacgcgaa gaatacggtc tatttgcaga tgaatagcct gaaaccggaa
     1861 gataccgcgg tttactactg taacgtgaaa gacagcggta gctccagcag ctcctacgac
     1921 tattggggcc aaggtaccca agtgactgtg agcgcataac tagaacaaaa actcatctca
     1981 gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttaaacggtc
     2041 tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     2101 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     2161 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     2221 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     2281 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     2341 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     2401 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     2461 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     2821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3241 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3721 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4261 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4321 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagtgc gcctgatgcg
     4381 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     4441 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     4501 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4561 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4621 ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag
     4681 cggcatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
     4741 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
     4801 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
     4861 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
     4921 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
     4981 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
     5041 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
     5101 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
     5161 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
     5221 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
     5281 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
     5341 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
     5401 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
     5461 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
     5521 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
     5581 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
     5641 tgcgcttcag ccatactttt catactcccg ccattcagag
//
