LOCUS       Exported                4987 bp ds-DNA     circular SYN 09-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CCR5-mTFP1
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 30, 2018 from SnapGene Viewer 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             592..1616
                     /codon_start=1
                     /label=hCCR5
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
                     /translation="SATGLRSRAQAYGGDQYIHFR*LHRGNGLRGL*LHEGTLFP*RKC
                     *FQ*NLPAHHLLHHLLNWHCGQWIGHPGHGLPEETEKHDGQVQAAPVSGRPPLCHHASL
                     LGS*CRGKLVLWELPMQGSPCHLHSQPLQQCPHPGLHQSGPLPGHRPRHQQSEAKEAVG
                     *KGGLCWRLDPCPPADYSRLHLCQRQ*GR*QIYL*PLLPQ*LVGGCVPVSAHHGWPYPA
                     WYCHPVLLLHYHLQAVTLQGPPEAQGPQDHSHPHPGFLRLLAALLHWDQHRLLHPPGNH
                     QARV*V*EHCAQVDFHHRGPSFLPLLSEPHPLCFPWSQI*NLCPARTHLCEQRVQPQDP
                     L"
     primer_bind     604..618
                     /label=Tandem_Seq1
     primer_bind     622..641
                     /label=hCCR5_For
     primer_bind     complement(1613..1630)
                     /label=hCCR5_Rev
     misc_feature    1623..1632
                     /label=Kozak
     CDS             1629..2336
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(2316..2336)
                     /label=TEV[RS]_R2
     primer_bind     complement(2328..2355)
                     /label=TEV[RS]_R
     primer_bind     2341..2355
                     /label=Tandem_Seq1
     primer_bind     complement(2418..2427)
                     /label=Tandem_Seq2
     primer_bind     complement(2496..2512)
                     /label=EGFP C R
     polyA_signal    2546..2667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2668..3096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3149..3253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3254..4114
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4285..4873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttatg gaggggatca gtatatacac ttcagataac
      661 tacaccgagg aaatgggctc aggggactat gactccatga aggaaccctg tttccgtgaa
      721 gaaaatgcta atttcaataa aatcttcctg cccaccatct actccatcat cttcttaact
      781 ggcattgtgg gcaatggatt ggtcatcctg gtcatgggtt accagaagaa actgagaagc
      841 atgacggaca agtacaggct gcacctgtca gtggccgacc tcctctttgt catcacgctt
      901 cccttctggg cagttgatgc cgtggcaaac tggtactttg ggaacttcct atgcaaggca
      961 gtccatgtca tctacacagt caacctctac agcagtgtcc tcatcctggc cttcatcagt
     1021 ctggaccgct acctggccat cgtccacgcc accaacagtc agaggccaag gaagctgttg
     1081 gctgaaaagg tggtctatgt tggcgtctgg atccctgccc tcctgctgac tattcccgac
     1141 ttcatctttg ccaacgtcag tgaggcagat gacagatata tctgtgaccg cttctacccc
     1201 aatgacttgt gggtggttgt gttccagttt cagcacatca tggttggcct tatcctgcct
     1261 ggtattgtca tcctgtcctg ctattgcatt atcatctcca agctgtcaca ctccaagggc
     1321 caccagaagc gcaaggccct caagaccaca gtcatcctca tcctggcttt cttcgcctgt
     1381 tggctgcctt actacattgg gatcagcatc gactccttca tcctcctgga aatcatcaag
     1441 caagggtgtg agtttgagaa cactgtgcac aagtggattt ccatcaccga ggccctagct
     1501 ttcttccact gttgtctgaa ccccatcctc tatgctttcc ttggagccaa atttaaaacc
     1561 tctgcccagc acgcactcac ctctgtgagc agagggtcca gcctcaagat cctctaccgg
     1621 tcgccaccat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga
     1681 agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg
     1741 gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc
     1801 ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca
     1861 ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt
     1921 gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca
     1981 tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg
     2041 gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc
     2101 gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
     2161 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
     2221 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
     2281 tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagtccg
     2341 gactcagatc tcgagtcgga tcctcgaatt cggtaccctc tagacaagct tgcggccgct
     2401 aactagctga gcatgccgat ccaccggatc tagataactg atcataatca gccataccac
     2461 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2521 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2641 tttgtccaaa ctcatcaatg tatcttaacg cgtcctgtag cggcgcatta agcgcggcgg
     2701 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2761 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2821 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2881 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2941 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3001 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3061 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3121 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3481 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4981 catgcat
//
