LOCUS       Exported                5740 bp ds-DNA     circular SYN 14-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CXCR4-pLSSmO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Dan
  TITLE     Direct Submission
  JOURNAL   Exported Mar 30, 2018 from SnapGene Viewer 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    628..1621
                     /label=CXCR4
     misc_feature    1629..1638
                     /label=Kozak
     primer_bind     1634..1655
                     /label=LSSmO_F
     CDS             1635..2342
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2289..2310)
                     /label=LSSmO(D11aa)_R
     primer_bind     complement(2322..2342)
                     /label=LSSmO_R
     misc_feature    2348..2404
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2528..2649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2656..3111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3138..3242
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3244..3601
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3452..3587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3636..4430
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4662..4709
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5038..5626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttatg gaggggatca gtatatacac ttcagataac
      661 tacaccgagg aaatgggctc aggggactat gactccatga aggaaccctg tttccgtgaa
      721 gaaaatgcta atttcaataa aatcttcctg cccaccatct actccatcat cttcttaact
      781 ggcattgtgg gcaatggatt ggtcatcctg gtcatgggtt accagaagaa actgagaagc
      841 atgacggaca agtacaggct gcacctgtca gtggccgacc tcctctttgt catcacgctt
      901 cccttctggg cagttgatgc cgtggcaaac tggtactttg ggaacttcct atgcaaggca
      961 gtccatgtca tctacacagt caacctctac agcagtgtcc tcatcctggc cttcatcagt
     1021 ctggaccgct acctggccat cgtccacgcc accaacagtc agaggccaag gaagctgttg
     1081 gctgaaaagg tggtctatgt tggcgtctgg atccctgccc tcctgctgac tattcccgac
     1141 ttcatctttg ccaacgtcag tgaggcagat gacagatata tctgtgaccg cttctacccc
     1201 aatgacttgt gggtggttgt gttccagttt cagcacatca tggttggcct tatcctgcct
     1261 ggtattgtca tcctgtcctg ctattgcatt atcatctcca agctgtcaca ctccaagggc
     1321 caccagaagc gcaaggccct caagaccaca gtcatcctca tcctggcttt cttcgcctgt
     1381 tggctgcctt actacattgg gatcagcatc gactccttca tcctcctgga aatcatcaag
     1441 caagggtgtg agtttgagaa cactgtgcac aagtggattt ccatcaccga ggccctagct
     1501 ttcttccact gttgtctgaa ccccatcctc tatgctttcc ttggagccaa atttaaaacc
     1561 tctgcccagc acgcactcac ctctgtgagc agagggtcca gcctcaagat cctctggatc
     1621 caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc atcaaggagt
     1681 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1741 gcgagggcga gggccgcccc tacgagggct ttcagaccgt taagctgaag gtgaccaagg
     1801 gtggccccct gcccttcgcc tgggacatct tgtcccctca gttcacctac ggctccaagg
     1861 cctacgtgaa gcaccccgcc gacatccccg actacctcaa gctgtccttc cccgagggct
     1921 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg actcaggact
     1981 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2041 ccgacggccc cgtaatgcag aagaagacca tgggcatgga ggcctcctcc gagcggatgt
     2101 accccgagga cggcgccctg aagggcgagg acaagctcag gctgaagctg aaggacggcg
     2161 gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg cagttgcccg
     2221 gcgcctacat cgtcgacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2281 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2341 agtactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     2401 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     2461 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2521 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2641 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     3121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3241 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3601 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     3661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3781 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     3841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4141 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     4201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4441 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4501 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4561 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     4621 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     4681 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4741 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4801 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4861 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     4921 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5161 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5701 atcccctgat tctgtggata accgtattac cgccatgcat
//
