LOCUS       Exported                5018 bp ds-DNA     circular SYN 31-MAY-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pTK-ENH_BsmB1-Avi-MCS-2A-Citrine.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2018 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..154
                     /note="Upstream polyA / transcriptional pause"
                     /note="color: #1ecc4f"
     misc_feature    162..664
                     /note="MCS"
                     /note="color: #000810"
     misc_feature    complement(212..487)
                     /label=lacZ'
                     /note="color: #ffb676; direction: LEFT"
     promoter        complement(488..609)
                     /label=lacZ promoter
                     /note="color: #f6fff8; direction: LEFT"
     misc_feature    682..937
                     /note="HSV-tk basal promoter"
                     /note="color: #0080ff"
     misc_feature    988..1120
                     /note="chimeric intron"
                     /note="color: #1926e7"
     misc_feature    1189..1233
                     /note="Avi Tag (biotinylation)"
                     /note="color: #2159ff"
     misc_feature    1240..1257
                     /note="TeV recognition peptide"
                     /note="color: #1bffff"
     misc_feature    1264..1287
                     /note="FLAG tag"
                     /note="color: #ffef5f"
     misc_feature    1294..1356
                     /label=TaV-2a
                     /note="color: #0080ff"
     primer_bind     1296..1317
                     /label=Primer 3
                     /note="color: black; sequence: 
                     TGCAGGCCAAGTGGCCGGCGCAGAGGCCTggatc AGGAGAGGGCAGGGGAAGT"
     misc_feature    1372..2091
                     /note="Citrine"
                     /note="color: #13ffb3; direction: RIGHT"
     misc_feature    2133..2354
                     /note="SV40 late polyA region"
                     /note="color: #ebfeed"
     misc_feature    complement(3444..4301)
                     /note="Beta-lactamase AmpR gene"
                     /note="color: #bff7ff; direction: LEFT"
     misc_feature    4434..4888
                     /note="f1 origin"
                     /note="color: #fecaec"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcgagttgc
      181 cagggagacg ggcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
      241 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      301 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      361 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
      421 tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
      481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      601 gcgttgcgct cactgcccgc tttccaccgg tcgtctcctg ttggctagcc cgggctcgag
      661 atctgcgatc taagtaagct tgctgcttca tccccgtggc ccgttgctcg cgtttgctgg
      721 cggtgtcccc ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc
      781 ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca
      841 ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg
      901 acccgcttaa cagcgtcaac agcgtgccgc agatcccaag cttgcggccg cttaagctgc
      961 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     1021 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     1081 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccaggttcaa
     1141 tacagctctt aagcggccgc aagctagctt gccgccacca tggctggtgg cctgaatgac
     1201 atctttgagg cccagaagat cgagtggcat gagggaggag agaacctgta cttccaggga
     1261 ggagactaca aggacgacga cgacaaggat atcggatcag gagagggcag gggaagtctt
     1321 ctaacatgcg gggacgtgga ggaaaatccc gggcccggcg gatccggagg catggtgagc
     1381 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1441 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1501 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1561 accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1621 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1681 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1741 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1801 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1861 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1921 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1981 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2041 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg
     2101 actctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2161 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2221 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2281 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2341 ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga gccttcaacc
     2401 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     2461 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact
     2521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     2881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     4441 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4501 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4561 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4621 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4681 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4741 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4801 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4861 tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg caactgttgg
     4921 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta
     4981 agtaatatta aggtacggga ggtacttgga gcggccgc
//
