LOCUS Exported 7423 bp ds-DNA circular SYN 04-ABR-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pD015-pQC MCS IRES G418 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7423) AUTHORS Douglas Adamoski TITLE Direct Submission JOURNAL Exported quarta-feira, 4 de abr de 2018 from SnapGene 2.8.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7423 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 309..512 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" LTR 513..688 /note="5' LTR (truncated)" /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 751..950 /note="MMLV Psi" /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1151..1567 /note="gag (truncated)" /note="truncated MMLV gag gene lacking the start codon" enhancer 1604..1907 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 1908..2111 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_difference 1942 /note="A-to-G" misc_feature 2223..2225 /note="Insertion" CDS 2255..2324 /codon_start=1 /note="MCS" /translation="GSGSRATGCMEFKLQSIDGSGRG" misc_feature 2278 /note="5' A Overhang" misc_feature 2303 /note="3' A Overhang" misc_feature 2332..2904 /note="IRES" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 2918..3712 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" LTR 4129..4554 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" promoter 4820..5149 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 5000..5135 /note="SV40 ori" /note="SV40 origin of replication" rep_origin complement(5442..6030) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6201..7061) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7062..7166) /gene="bla" /note="AmpR promoter" enhancer 7352..308 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 2221 cggacctgca ggaattgatc cgcggccgca ccggggatcc ggttctagag ccaccggttg 2281 tatggagttt aaactccaaa gcatcgatgg tagcggccgc ggtgaattcc gcccctctcc 2341 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt 2401 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg 2461 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg 2521 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc 2581 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca 2641 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag 2701 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa 2761 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt 2821 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 2881 ttcctttgaa aaacacgatg ataagcttgc cacaaccatg gctgaacaag atggattgca 2941 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 3001 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 3061 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 3121 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 3181 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 3241 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 3301 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 3361 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 3421 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 3481 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 3541 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 3601 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 3661 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggatc 3721 ggctagagtc gacccggacg ggacgctcct gcgcctgata cagaacgaat tgcttgcagg 3781 catctcatga gtgtgtcttc ccgttttccg cctgaggtca ctgcgtggat ggagcgctgg 3841 cgcctgctgc gcgacggcga gctgctcacc acccactcga gggctgcagc gctgcagagg 3901 ccgagtgcag aactgctcca aagggacctc aaggctttcc gagggacact aggctgactc 3961 catcgagcca gtgtagagat aagcttatcg attagtccaa tttgttaaag acaggatatc 4021 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga 4081 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc 4141 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac 4201 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ggtcgaccct 4261 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt 4321 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca 4381 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg 4441 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc 4501 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg 4561 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc 4621 tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 4681 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 4741 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 4801 gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 4861 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 4921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 4981 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 5041 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 5101 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactgg 5161 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 5221 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 5281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 5341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 5401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 5461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 5521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 5581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 5641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 5701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 5761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 5821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 5881 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 5941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 6181 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 6241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 6301 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 6361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 6421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 6481 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 6541 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 6601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 6661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 6721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 6781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 6841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 6901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 6961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 7201 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 7261 cttcaagaat tagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat 7321 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag 7381 taatcaatta cggggtcatt agttcatagc ccatatatgg agt //