LOCUS       Exported                1878 bp ds-DNA     linear   SYN 23-JUL-2008
DEFINITION  Synthetic construct for chimaeric Homo sapiens NRAS gene, partial 5'
            UTR region and Photinus pyralis luc gene.
ACCESSION   AM489487
VERSION     .
KEYWORDS    luc gene; luciferase; NRAS gene.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Kumari S, Bugaut A, Huppert JL, Balasubramanian S.
  TITLE     An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene 
            modulates translation
  JOURNAL   Nat. Chem. Biol. 3 (4), 218-221 (2007)
  PUBMED    17322877
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Kumari S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2007) Kumari S., Chemistry Department, University 
            of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UNITED KINGDOM
REFERENCE   3  (bases 1 to 1878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 5, 2018 from SnapGene Viewer 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="synthetic construct"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
     source          1..225
                     /organism="Homo sapiens"
                     /mol_type="other DNA"
                     /db_xref="taxon:9606"
     source          226..1878
                     /organism="Photinus pyralis"
                     /mol_type="other DNA"
                     /db_xref="taxon:7054"
     gene            1..225
                     /gene="NRAS"
                     /label=NRAS
     5'UTR           1..225
                     /gene="NRAS"
                     /label=deleted 5'UTR sequence
                     /note="deleted 5'UTR sequence"
     gene            226..1878
                     /gene="luc"
                     /label=luc
     CDS             226..1878
                     /codon_start=1
                     /gene="luc"
                     /product="luciferase"
                     /label=luc
                     /protein_id="CAM31945.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gggtgcggcc tgccgcatga ctcgtggttc ggaggcccac gtggccgggg cggggactca
       61 ggcgcctggg gcgccgactg attacgtagc gggcggggcc ggaagtgccg ctccttggtg
      121 ggggctgttc atggcggttc cggggtctcc aacatttttc ccggctgtgg tcctaaatct
      181 gtccaaagca gaggcagtgg agcttgaggt tcttgctggt gtgaaatgga agacgccaaa
      241 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
      301 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      361 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
      421 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
      481 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
      541 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
      601 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
      661 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
      721 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
      781 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
      841 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
      901 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
      961 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     1021 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg
     1081 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     1141 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     1201 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     1261 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     1321 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     1381 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     1441 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     1501 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     1561 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     1621 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     1681 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     1741 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     1801 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     1861 ggaaagatcg ccgtgtaa
//
