LOCUS       pBTK614                 6699 bp ds-DNA     circular     20-SEP-2017
DEFINITION  .
ACCESSION   urn.local...ai-82xqzyj
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    16..158
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="Con1"
     misc_feature    166..169
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="Con1 scar"
     promoter        199..346
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="KanR Promoter"
     CDS             347..4453
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /modified_by="seanleonard"
                     /label="dCas9"
     misc_feature    4458..4652
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="Terminator-M13"
     misc_feature    4668..4810
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="ConE"
     terminator      complement(4838..4934)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="AmpR Terminator"
     misc_feature    complement(4935..5792)
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
                     /label="AmpR"
     promoter        complement(5793..5923)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="AmpR Promoter"
     rep_origin      complement(5924..6687)
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /label="ColE1"
ORIGIN      
        1 gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat
       61 acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc cacctaagcc
      121 tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaac caaaccagat
      181 gtcaacacag ctacaacgct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag
      241 ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
      301 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatgg ataagaaata
      361 ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata
      421 taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa
      481 aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctaaa
      541 acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat
      601 tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt
      661 tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga
      721 agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc
      781 tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg
      841 tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt
      901 tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg
      961 agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct
     1021 cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc
     1081 attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca
     1141 gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca
     1201 atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat
     1261 cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta
     1321 cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga
     1381 aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg
     1441 gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg
     1501 tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt
     1561 tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag
     1621 acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac
     1681 ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat
     1741 gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg
     1801 tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga
     1861 aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac
     1921 aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa
     1981 gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa
     2041 agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga
     2101 tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga
     2161 ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt
     2221 atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga
     2281 taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa
     2341 attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc
     2401 agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa
     2461 agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc
     2521 aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga
     2581 tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg
     2641 tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga
     2701 agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca
     2761 attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga
     2821 ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg ttccacaaag
     2881 tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg
     2941 taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca
     3001 acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg
     3061 tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg
     3121 ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga
     3181 aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga
     3241 cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca
     3301 tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga
     3361 atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc
     3421 tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt
     3481 cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac
     3541 taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa
     3601 agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt
     3661 ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga
     3721 ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt
     3781 ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg
     3841 gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa
     3901 aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga
     3961 gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga
     4021 gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt
     4081 gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta
     4141 tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc
     4201 caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca
     4261 agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa
     4321 atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc
     4381 cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct
     4441 aggaggtgac tgaatccaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga
     4501 gccttttttt ttggagattt tcaacatgaa gaaattatta tttgatgatc agatagcggc
     4561 ggggaactgc cagacatcaa ataaaacaaa aggctcagtc ggaagactgg gccttttgtt
     4621 ttatctgttg tttgtcggtg aacactctcc cggctgagca tgagacggaa atctgctcgt
     4681 cagtggtgct cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca
     4741 caactaagca agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca
     4801 caacagtcca actagtgcac tgcagtacag cggccgcgat tatcaaaaag gatcttcacc
     4861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5041 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     5101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct
     5941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6661 ttatcccctg attctgtgga taaccgtgcg gccgcccct
//
