LOCUS       MSP1594_2x_NLS.xdna    8282 bp      DNA     circular   2018-04-09
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: pCAG-CFP. - [LOCUS]: MSP1594 - [ACCESSION]: ur
            n.local...k-4oqvoi9 - [CDS]: 1769..5134
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        18..1718
                     /label=CAG promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="CAG promoter" -  \ 
                     /SerialCloner_Desc=18..1718 \ 
     promoter        18..381
                     /label=CMV Enhancer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=SerialCloner_Color="&h03C000" - SerialCloner_Show="True" - SerialCloner_Protect="True" - SerialCloner_Arrow="True" - Color="#ff5555" - modified_by="User" - X-Transferred_From="MSP469; MSP469 updated; MSP680; - MSP977; pCMV-T7-Cas9 (MLM3613); pCMV-T7-Cas9  \ 
     misc_feature    1763..1772
                     /label=Kozak
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - modified_by="User" - X-Transferred_From="BPK2139; MSP680; MSP977" - X-Transferred_Similarity="100.00%" - Transferred_From="BPK2139, MSP1101, MSP680, MSP977" - Transferred_Similarity="100.00%" - label="Kozak" -  \ 
                     /SerialCloner_Desc=1763..1772 \ 
     misc_feature    5195..5215
                     /label=SV40 NLS
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 NLS" -  \ 
                     /SerialCloner_Desc=5147..5167 \ 
     terminator      5236..5739
                     /label=rb glob PA terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     misc_feature    complement(5803..5830)
                     /label=Geneious type: regulatory sequence_lacO reg
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Imported_from="<a - /wishart.biology.ualberta.ca/PlasMapper"">Pla - sMapper" - X-Transferred_From="pCAG-hCas9-nls-3XFLAG (SQT817); - pCAG-hCas9-nls-3XFLAG delta NcoI (BPK742); pCMV-T7-Cas9 - (MLM3613); pCMV-T7-Cas9 delta NcoI" - X-Transferred_Similarity=" \ 
     promoter        complement(5834..5863)
                     /label=lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     unsure          5969..6148
                     /label=SV40 promoter
                     /SerialCloner_Color=&hFFD700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - label="SV40 promoter" -  \ 
                     /SerialCloner_Desc=5921..6100 \ 
     unsure          6004..6081
                     /label=SV40 origin
                     /SerialCloner_Color=&hFFD700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=created_by="User" - Color="#aa00aa" - modified_by="User" - label="SV40 origin" -  \ 
                     /SerialCloner_Desc=5956..6033 \ 
     terminator      6162..6293
                     /label=SV40 PA terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     rep_origin      complement(6517..7136)
                     /label=pBR322 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT - LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK - DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN - GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH - MVLLEFVTAAGI \ 
     cds             complement(7291..8151)
                     /label=ampR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Imported_from="<a - /wishart.biology.ualberta.ca/PlasMapper"">Pla - sMapper" - modified_by="User" - created_by="User" - X-Transferred_From="BPK1520; BPK2301; MSP469; MSP680; - MSP977; VVT1; pCAG-hCas9-nls-3XFLAG (SQT817); - pCAG-hCas9-nls-3XFLAG delta Nco \ 
     promoter        complement(8193..8221)
                     /label=amp promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE - YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL - LRSALPAGWFIA \ 
     misc_feature    1775..1822
                     /label=SV40 NLS + Llinker
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="APKKKRKVGIHGVPAA" \ 
     misc_feature    1823..5182
                     /label=StCas9 LMD-9
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVRRTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEELFIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDE \ 
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc 
     1741 gcgggcccgg gatccaccgg tcgccaccat gggcGCCCCA AAGAAGAAGC GGAAGGTCGG 
     1801 TATCCACGGA GTCCCAGCAG CCagcgacct ggtgctgggc ctggacatcg gcatcggcag 
     1861 cgtgggcgtg ggcatcctga acaaggtgac cggcgagatc atccacaaga acagtcgcat 
     1921 cttccctgct gctcaggctg agaacaacct ggtgcgccgc accaaccgcc agggtcgccg 
     1981 gcttgctcgc cgcaagaagc accggcgcgt gcgcctgaac cgcctgttcg aggagagcgg 
     2041 cctgatcacc gacttcacca agatcagcat caacctgaac ccctaccagc tgcgcgtgaa 
     2101 gggcctgacc gacgagctga gcaacgagga gctgttcatc gccctgaaga acatggtgaa 
     2161 gcaccgcggc atcagctacc tggacgacgc cagcgacgac ggcaacagca gcgtgggcga 
     2221 ctacgcccag atcgtgaagg agaacagcaa gcagctggag accaagaccc ccggccagat 
     2281 ccagctggag cgctaccaga cctacggcca gctgcgcggc gacttcaccg tggagaagga 
     2341 cggcaagaag caccgcctga tcaacgtgtt ccccaccagc gcctaccgca gcgaggccct 
     2401 gcgcatcctg cagacccagc aggagttcaa cccccagatc accgacgagt tcatcaaccg 
     2461 ctacctggag atcctgaccg gcaagcgcaa gtactaccac ggccccggca acgagaagag 
     2521 ccgcaccgac tacggccgct accgcaccag cggcgagacc ctggacaaca tcttcggcat 
     2581 cctgatcggc aagtgcacct tctaccccga cgagttccgc gccgccaagg ccagctacac 
     2641 cgcccaggag ttcaatctgc tgaacgacct gaacaacctg accgtgccca ccgagaccaa 
     2701 gaagctgagc aaggagcaga agaaccagat catcaactac gtgaagaacg agaaggctat 
     2761 gggccccgcc aagctgttca agtacatcgc caagctgctg agctgcgacg tggccgacat 
     2821 caagggctac cgcatcgaca agagcggcaa ggccgagatc cacaccttcg aggcctaccg 
     2881 caagatgaag accctggaga ccctggacat cgagcagatg gaccgagaga ccctggacaa 
     2941 gctggcctac gtgctgaccc tgaacaccga gcgcgagggc atccaggagg ccctggagca 
     3001 cgagttcgcc gacggcagct tcagccagaa acaggtggac gagctggtgc agttccgcaa 
     3061 ggccaacagc agcatcttcg gcaagggctg gcacaacttc agcgtgaagc tgatgatgga 
     3121 gctgatcccc gagctgtacg agaccagcga ggagcagatg accatcctga cccgcctggg 
     3181 caagcagaag accaccagca gcagcaacaa gaccaagtac atcgacgaga agctgctgac 
     3241 cgaggagatc tacaaccccg tggtggccaa gagcgtgcgc caggccatca agatcgtgaa 
     3301 cgccgccatc aaggagtacg gcgacttcga caacatcgtg atcgagatgg cccgcgagac 
     3361 caacgaggac gacgagaaga aggccatcca gaagatccag aaggccaaca aggacgagaa 
     3421 ggacgccgcc atgctgaagg ccgccaacca gtacaacggc aaggccgagc tgccccacag 
     3481 cgtgttccac ggccacaagc agctggccac caagatccgc ctgtggcacc agcagggcga 
     3541 gcgctgcctg tacaccggca agaccatcag catccacgac ctgatcaaca acagcaacca 
     3601 gttcgaggtg gaccacatcc tgcccctgag catcaccttc gacgacagcc tggccaacaa 
     3661 ggtgctggtg tacgccaccg ccaaccagga gaagggccag cgcaccccct accaggccct 
     3721 ggacagcatg gacgacgcct ggagcttccg cgagctgaag gccttcgtgc gcgagagcaa 
     3781 gaccctgagc aacaagaaga aggagtatct gctgaccgag gaggacatca gcaagttcga 
     3841 cgtgcgcaag aagttcatcg agcgcaacct ggtggacacc cgctacgcca gccgcgtggt 
     3901 gctgaacgcc ctgcaggagc acttccgcgc ccacaagatc gacaccaagg tgagcgtggt 
     3961 gcgcggccag ttcaccagcc agctgcgccg ccactggggc atcgagaaga cccgcgacac 
     4021 ctaccaccac cacgccgtgg acgccctgat cattgcggct tctagccagc tgaacctgtg 
     4081 gaagaagcag aagaacaccc tggtgagcta cagcgaggac cagctgctgg acatcgagac 
     4141 cggcgagctg atcagcgacg acgagtacaa ggagagcgtg ttcaaggccc cctaccagca 
     4201 cttcgtggac accctgaaga gcaaggagtt cgaggacagc atcctgttca gctaccaggt 
     4261 ggacagcaag ttcaaccgca agatcagcga cgccaccatc tacgccaccc gccaggccaa 
     4321 ggtgggcaag gacaaggccg acgagaccta cgtgctgggc aagatcaagg acatctacac 
     4381 ccaggacggc tacgacgcct tcatgaagat ctacaagaag gacaagagca agttcctgat 
     4441 gtaccgccac gacccccaga ccttcgagaa ggtgatcgag cccatcctgg agaactaccc 
     4501 caacaagcag atcaacgaga aaggcaagga ggtgccctgc aaccccttcc tgaagtacaa 
     4561 ggaggagcac ggctacatcc gcaagtacag caagaagggc aacggccccg agatcaagag 
     4621 cctgaagtac tacgacagca agctgggcaa ccacatcgac atcaccccca aggacagcaa 
     4681 caacaaggtg gtgctgcaga gcgtgagccc ctggcgcgcc gacgtgtact tcaacaagac 
     4741 caccggcaag tacgagatcc tggggctgaa gtacgccgat ctgcagtttg agaaaggcac 
     4801 aggcacctac aagatcagcc aggagaagta caacgacatc aagaagaagg agggcgtgga 
     4861 cagcgacagc gagttcaagt tcaccctgta caagaacgac cttctgctgg tgaaggacac 
     4921 cgagaccaag gagcaacagc tgttccgctt cctgagccgc accatgccca agcagaagca 
     4981 ctacgtggag ctgaagccct acgacaagca gaagttcgag ggcggcgagg ccctgatcaa 
     5041 ggtgctgggc aacgtggcca acagcggcca gtgcaagaag ggcctgggca agagcaacat 
     5101 cagcatctac aaggtgcgca ccgacgtgct gggcaaccag cacatcatca agaacgaggg 
     5161 cgacaagccc aagttggact tcagcagggc tgaccccaag aagaagagga aggtgtgagc 
     5221 ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc 
     5281 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg 
     5341 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg 
     5401 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa 
     5461 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg 
     5521 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc 
     5581 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta 
     5641 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc 
     5701 ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg 
     5761 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 
     5821 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 
     5881 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 
     5941 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 
     6001 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
     6061 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
     6121 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata 
     6181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     6241 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca 
     6301 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     6361 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     6421 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     6481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     6541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     6601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     6661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     6721 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     6781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     6841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     6901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     6961 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     7021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     7081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     7141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     7201 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     7261 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     7321 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     7381 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     7441 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     7501 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     7561 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     7621 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     7681 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     7741 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     7801 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     7861 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     7921 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     7981 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     8041 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     8101 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     8161 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     8221 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     8281 tg
//