LOCUS       pUPATrap_VC_ORF0        8144 bp ds-DNA     circular     10-APR-2018
DEFINITION  Gene-trapping transposon vector pUPATrap-TMat-14 DNA, complete
            sequence.
ACCESSION   AB673359
VERSION     AB673359.1  GI:380035599
KEYWORDS    .
SOURCE      Gene-trapping transposon vector pUPATrap-TMat-14
  ORGANISM  Gene-trapping transposon vector pUPATrap-TMat-14 other sequences;
            artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Mayasari,N.I., Mukougawa,K., Shigeoka,T., Kawakami,K., Kawaichi,M.
            and Ishida,Y.
  TITLE     Mixture of differentially tagged Tol2 transposons accelerates
            conditional disruption of a broad spectrum of genes in mouse
            embryonic stem cells
  JOURNAL   Nucleic Acids Res. 40 (13), E97 (2012)
   PUBMED   22447447
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Ishida,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2011) Contact:Yasumasa Ishida Nara Institute of
            Science and Technology, Graduate School of Biological Sciences;
            8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(1..2203,3634..6697,6704..8144)
                     /organism="Gene-trapping transposon vector
                     pUPATrap-TMat-14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1091348"
                     /label=source:Gene-trapping transposon vector pUPATrap-TM
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          2212..3242
                     /organism="Gene-trapping transposon vector
                     pUPATrap-TMat-14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1091348"
                     /label=source:Gene-trapping transposon vector
                     pUPATrap-TM(1)
                     /ApEinfo_label=source:Gene-trapping transposon vector
                     pUPATrap-TM
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            125..985
                     /gene="bla"
                     /label=bla
                     /ApEinfo_fwdcolor=#ffe511
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          3243..3260
                     /organism="Gene-trapping transposon vector
                     pUPATrap-TMat-14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1091348"
                     /label=source:Gene-trapping transposon vector
                     pUPATrap-TM(2)
                     /ApEinfo_label=source:Gene-trapping transposon vector
                     pUPATrap-TM
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             125..985
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance protein"
                     /protein_id="BAL72145.1"
                     /db_xref="GI:380035600"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=ampicillin resistance protein
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3578..3599
                     /label=EGFP_C_primer
                     /ApEinfo_fwdcolor=#ff4000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2272..2471
                     /note="L200 (an essential Tol2-transposon sequence)"
                     /label=L200 (an essential Tol2-transposon sequence)
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          3261..3633
                     /organism="pBiFC-VC155"
                     /mol_type="other DNA"
                     /label=source:pBiFC-VC155
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2511..2544
                     /note="FRT site; Flp recognition site"
                     /label=FRT site; Flp recognition site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            3396..3633
                     /gene="ECFP (468 - 713)"
                     /label=ECFP (468 - 713)
                     /ApEinfo_fwdcolor=#38f6ff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2664..2697
                     /note="F3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT) site"
                     /label=F3 site; synthetic variant 3 of the yeast flp-reco
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2756..2789
                     /note="loxP"
                     /label=loxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2966..2999
                     /note="lox5171"
                     /label=lox5171
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3634..3644
                     /gene="EGFP"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="BAL72146.1"
                     /db_xref="GI:380035601"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
                     /label=enhanced green fluorescent protein
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3243..3260
                     /note="the human bcl-2 gene splice acceptor (intron 2 -
                     modified exon 3)"
                     /label=the human bcl-2 gene splice acceptor (intron 2 - m
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3665..3914
                     /note="the bovine growth hormone gene poly(A)-addition
                     signal #1"
                     /label=the bovine growth hormone gene poly(A)-addition si
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3006..3242
                     /note="the human bcl-2 gene splice acceptor (intron 2 -
                     modified exon 3)"
                     /label=the human bcl-2 gene splice acceptor (intron 2 -
                     m(1)
                     /ApEinfo_label=the human bcl-2 gene splice acceptor
                     (intron 2 - m
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3918..4169
                     /note="the bovine growth hormone gene poly(A)-addition
                     signal #2"
                     /label=the bovine growth hormone gene poly(A)-addition
                     si(1)
                     /ApEinfo_label=the bovine growth hormone gene
                     poly(A)-addition si
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     4173..4479
                     /note="the human growth hormone gene poly(A)-addition
                     signal #1"
                     /label=the human growth hormone gene poly(A)-addition sig
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     4494..4807
                     /note="the human growth hormone gene poly(A)-addition
                     signal #2"
                     /label=the human growth hormone gene poly(A)-addition
                     sig(1)
                     /ApEinfo_label=the human growth hormone gene
                     poly(A)-addition sig
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3261..3287
                     /label=HA-tag
                     /ApEinfo_fwdcolor=#ffe912
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4815..4848)
                     /note="loxP"
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3243..3260
                     /label=Bcl-2 exon2
                     /ApEinfo_fwdcolor=#1bd5ff
                     /ApEinfo_revcolor=#46ff92
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(5023..5056)
                     /note="lox5171"
                     /label=lox5171(1)
                     /ApEinfo_label=lox5171
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(5102..5135)
                     /note="FRT site; Flp recognition site"
                     /label=FRT site; Flp recognition site(1)
                     /ApEinfo_label=FRT site; Flp recognition site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5174..5875
                     /note="the mouse RNA polymerase II (the RPB1 subunit) gene
                     promoter"
                     /label=the mouse RNA polymerase II (the RPB1 subunit) gen
                     /ApEinfo_fwdcolor=#0cff35
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     6707..6797
                     /note="a synthetic base sequence for 3' RACE PCR"
                     /label=a synthetic base sequence for 3' RACE PCR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6804..7025
                     /note="the mouse hprt gene splice donor (modified exon 8 -
                     intron 8)"
                     /label=the mouse hprt gene splice donor (modified exon 8 
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(7035..7068)
                     /note="F3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT) site"
                     /label=F3 site; synthetic variant 3 of the yeast
                     flp-reco(1)
                     /ApEinfo_label=F3 site; synthetic variant 3 of the yeast
                     flp-reco
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7078..7165
                     /note="a synthetic base sequence for splinkerette genome
                     PCR"
                     /label=a synthetic base sequence for splinkerette genome 
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7174..7209
                     /note="the CC-in-poly(AT) sequence #14"
                     /label=the CC-in-poly(AT) sequence #14
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7213..7242
                     /note="the vector-ID sequence #14"
                     /label=the vector-ID sequence #14
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7257..7291
                     /note="the Term sequence"
                     /label=the Term sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            3634..3644
                     /gene="EGFP"
                     /label=EGFP
                     /ApEinfo_fwdcolor=#09ff0d
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7292..7466
                     /note="R175 (an essential Tol2-transposon sequence)"
                     /label=R175 (an essential Tol2-transposon sequence)
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2168..2187
                     /label=T3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(7525..7542)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2130..2150
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(7496..7516)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      1080..1762
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      7700..8006
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             7613..7681
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    2102..2124
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2511..2544
                     /label=FRT
                     /ApEinfo_fwdcolor=orchid
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(5102..5135)
                     /label=FRT(1)
                     /ApEinfo_label=FRT
                     /ApEinfo_fwdcolor=orchid
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2756..2789
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4815..4848)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             323..982
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7107..7466
                     /label=Tol2
                     /ApEinfo_fwdcolor=#ff9fd2
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7292..7466
                     /label=Tol2_3'end
                     /ApEinfo_fwdcolor=#ff181e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3261..3279
                     /label=HA-tag(1)
                     /ApEinfo_label=HA-tag
                     /ApEinfo_fwdcolor=#ffdb61
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3243..3260
                     /label=bcl2-5AA
                     /ApEinfo_fwdcolor=#f0a1ff
                     /ApEinfo_revcolor=#faa7ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3392..3414
                     /label=GSP1_GFP/VC_R
                     /ApEinfo_fwdcolor=#20ff64
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            5892..6695
                     /gene="Neo"
                     /label=Neo
                     /ApEinfo_fwdcolor=#ffe511
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7107..7136
                     /label=SPL-2m
                     /ApEinfo_fwdcolor=#ffef13
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7078..7106
                     /label=SPL-1
                     /ApEinfo_fwdcolor=#ffef13
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaGCTAG Ctggcggcgg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tgggcccggc tcgatctccg cggccgcatg atcgattgcc gaagttccta
     2521 ttctctagaa agtataggaa cttcggcatc ggcatcaatt catcatcgga tatttgcatc
     2581 gcaatatcaa tccattgccc ggatggccgg atgatcgatg gcggatgcca tgcgatggat
     2641 cggaaatcgc atccattccg gccgaagttc ctattcttca aatagtatag gaacttcggc
     2701 gaattcggaa tcattggcaa tgccatccat cggatttgat gccatgcaat tccggataac
     2761 ttcgtatagc atacattata cgaagttatc cgaaagctgt ccccaatcct cccaccacga
     2821 cccgccgcac tgcagatacc gcaccgtatt ggcaagtagc ccgtaaacgc ggcgaatcgc
     2881 agccagcata gccaggtcca gccgctcgcc ggggcgctgg cgtttggcca ggcggtcgat
     2941 gtgtctgtcc tccggaaggg cccggataac ttcgtatagt acacattata cgaagttatc
     3001 cgggatctgc tgtatctcta agatggctgg cacgtaaaga gggtctcaat aaatatgcct
     3061 tgaattaatg aaaacagtgg tttctgaatc atggcctcca aagagcattt gccttttccc
     3121 ctctcaccct tctgaaggtg cccaggctgc cctggcagga tgcctctttc tctgtggggt
     3181 ggcattctct gctctctctc taatactctt tctttttctt ccctcctctc ccccaactgc
     3241 agGATGCCTT TGTGGAACTG tacccatacg atgttccaga ttacGCTCTT ATGGCCATGG
     3301 AGGCCCGAAT TCGGTCGACC GAGATCTCTC GAGGTACCCG TCCGGCGTGC AAAATCCCGA
     3361 ACGACCTGAA ACAGAAAGTC ATGAACCACG Acaagcagaa gaacggcatc aaggCCAACT
     3421 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
     3481 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
     3541 CCAAACTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
     3601 CCGCCGCCGG GATCACTCTC GGcatggacg agctgtacaa gtaaagcgcc agcacagtgg
     3661 tcgagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3721 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3781 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3841 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3901 tgaggcggaa agctagactc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3961 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4021 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4081 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4141 tctatggctt ctgaggcgga aagcttcagc gatggcttct agctgcccgg gtggcatccc
     4201 tgtgacccct ccccagtgcc tcttctggcc ctggaagttg ccactccagt gcccaccagc
     4261 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     4321 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     4381 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     4441 tccgcctcct gggttcaagc gattctcctg cctcagcctt ctagcttcag cgatggcttc
     4501 tagctgcccg ggtggcatcc ctgtgacccc tccccagtgc ctcttctggc cctggaagtt
     4561 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4621 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     4681 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     4741 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     4801 cccgagtcga gcggataact tcgtataatg tatgctatac gaagttatcc gcgccaggtc
     4861 gcagatcgtc ggtatggagc cgggggtggt gacgtgggtc tggaccatcc cggaggtaag
     4921 ttgcagcagg gcgtcccggc agccggcggg cgattggtcg taatccagga taaagacgtg
     4981 catggaacgg aggcgtttgg ccaagacgtc caaggcccac ggataacttc gtataatgtg
     5041 tactatacga agttatccga tcgatatcca tcccaaatat gcgatggatg gatcggatgc
     5101 cgaagttcct atactttcta gagaatagga acttcggcat tgcggccgca aatagatcca
     5161 tccaatgaag cttgggctga acatcgagcg ccagggctcc gtaaagctac tagagcacag
     5221 gcggtgcccc aacgtcctgg ggcctctcca ctaataacgg ctacttccaa ttgattggac
     5281 gcgccatctt gcctgcctta tgcatattca gcggtgaact gaatattcat gaacgaggcc
     5341 cgtcccgtcc ctccctcctt ccccccaccc ccggaacccg ctccggagga cccgaagggc
     5401 cccgccttca ttaccgatgc gtaggacaaa ccattttccc gatgtgtgtg gggggatact
     5461 aatgagagac tttagctgaa aaatgagcct gaactccgaa gctgagtaaa aatggcctaa
     5521 ctttatcctc cgttctgtaa gtcctcggtt tgagtgcacg ggaaacccga aaggaggacg
     5581 acaggaccag gaacattctc ctcctcctgt cgcgtcagaa agaacaccca accagggagc
     5641 cggagcccta gcgtcaacaa ctccgccgcg cgcgctccgt gtaggccggt gcgggcggcc
     5701 ccgtagcgca agggagggcg ggaaaggaag gggcgggaca caagggcgaa tctataaagg
     5761 gcgtcactca gccagttctc tcctcagaag cgccgagagc gcgaccggga cggttggaga
     5821 agaaggtggc tcccggaagg gggagagaca aactgccgta acctctgccg ttcaggatcc
     5881 gtgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     5941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6061 ccggtgcCCT GAATGAACTG CAGGACGagg cagcgcggct atcgtggctg gccacgacgg
     6121 gcgttccttg cgcagctgtg ctcgacGTTG TCACTGAAGC GGGAAGggac tggctgctat
     6181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6421 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     6481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6661 tcgccttcta tcGCCTTCTT GACGAGTTCT TCTGAccACC GGTgacgcca TGGCCAATCG
     6721 CATCATGGAT TCCatccgat gcgaatcgca tccatggatg cgccaatcgg attccatgcg
     6781 atgccatttc cgatgcggga tccgaaattc cagacaagtt tgttgttgga tatgccctcg
     6841 actacaacga gtacttcagg aatttgcagg taagtaatgc tttttttttt ctcgctctca
     6901 tttttcaaaa acccacataa aaattgagga aagggaagaa ttgttttctc cttccagcac
     6961 ctcgtaattt gacccgactg atggttccca ttagtcacat aaagctgtag tcaagtacag
     7021 acgtcgaatt cgccgaagtt cctatactat ttgaagaata ggaacttcgg cctcgagCAT
     7081 GCATCATATC CATCGCAATC GCATCCCCAT TCAATTCACA TCGCATATCC GATGCCGATA
     7141 TGGCATCGGC ATTGCCATCC AATCCTGATC Accatatata tGATGatata tatatGATGa
     7201 tatatatatA TTTTTCCATT GCATCATCCC CGATCATGCC ATATCCGAAT GGATCCATGC
     7261 GGATCATCGC ATCCATTGCG ATAAAGGATC TTTCTTGCTT TTACTTTTAC TTCCTTAATA
     7321 CTCAAGTACA ATTTTAATGG AGTACTTTTT TACTTTTACT CAAGTAAGAT TCTAGCCAGA
     7381 TACTTTTACT TTTAATTGAG TAAAATTTTC CCTAAGTACT TGTACTTTCA CTTGAGTAAA
     7441 ATTTTTGAGT ACTTTTTACA CCTCTGtcaa gaaccatatg ccggtaccca attcgcccta
     7501 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7681 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7741 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7861 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7981 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8041 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8101 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtgg 
//
