LOCUS       pUPATrap_VC_ORF1        8145 bp ds-DNA     circular     10-APR-2018
DEFINITION  Gene-trapping transposon vector pUPATrap-TMat-14 DNA, complete
            sequence.
ACCESSION   AB673359
VERSION     AB673359.1  GI:380035599
KEYWORDS    .
SOURCE      Gene-trapping transposon vector pUPATrap-TMat-14
  ORGANISM  Gene-trapping transposon vector pUPATrap-TMat-14 other sequences;
            artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Mayasari,N.I., Mukougawa,K., Shigeoka,T., Kawakami,K., Kawaichi,M.
            and Ishida,Y.
  TITLE     Mixture of differentially tagged Tol2 transposons accelerates
            conditional disruption of a broad spectrum of genes in mouse
            embryonic stem cells
  JOURNAL   Nucleic Acids Res. 40 (13), E97 (2012)
   PUBMED   22447447
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Ishida,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2011) Contact:Yasumasa Ishida Nara Institute of
            Science and Technology, Graduate School of Biological Sciences;
            8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(1..2203,3635..6698,6705..8145)
                     /organism="Gene-trapping transposon vector
                     pUPATrap-TMat-14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1091348"
                     /label=source:Gene-trapping transposon vector pUPATrap-TM
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          2212..3242
                     /organism="Gene-trapping transposon vector
                     pUPATrap-TMat-14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1091348"
                     /label=source:Gene-trapping transposon vector
                     pUPATrap-TM(1)
                     /ApEinfo_label=source:Gene-trapping transposon vector
                     pUPATrap-TM
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            125..985
                     /gene="bla"
                     /label=bla
                     /ApEinfo_fwdcolor=#ffe511
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          3243..3261
                     /organism="Gene-trapping transposon vector
                     pUPATrap-TMat-14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1091348"
                     /label=source:Gene-trapping transposon vector
                     pUPATrap-TM(2)
                     /ApEinfo_label=source:Gene-trapping transposon vector
                     pUPATrap-TM
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             125..985
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance protein"
                     /protein_id="BAL72145.1"
                     /db_xref="GI:380035600"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=ampicillin resistance protein
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3579..3600
                     /label=EGFP_C_primer
                     /ApEinfo_fwdcolor=#ff4000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2272..2471
                     /note="L200 (an essential Tol2-transposon sequence)"
                     /label=L200 (an essential Tol2-transposon sequence)
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          3262..3634
                     /organism="pBiFC-VC155"
                     /mol_type="other DNA"
                     /label=source:pBiFC-VC155
                     /ApEinfo_fwdcolor=#ffffff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2511..2544
                     /note="FRT site; Flp recognition site"
                     /label=FRT site; Flp recognition site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            3397..3634
                     /gene="ECFP (468 - 713)"
                     /label=ECFP (468 - 713)
                     /ApEinfo_fwdcolor=#38f6ff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2664..2697
                     /note="F3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT) site"
                     /label=F3 site; synthetic variant 3 of the yeast flp-reco
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2756..2789
                     /note="loxP"
                     /label=loxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2966..2999
                     /note="lox5171"
                     /label=lox5171
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3635..3645
                     /gene="EGFP"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="BAL72146.1"
                     /db_xref="GI:380035601"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
                     /label=enhanced green fluorescent protein
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3243..3261
                     /note="the human bcl-2 gene splice acceptor (intron 2 -
                     modified exon 3)"
                     /label=the human bcl-2 gene splice acceptor (intron 2 - m
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3666..3915
                     /note="the bovine growth hormone gene poly(A)-addition
                     signal #1"
                     /label=the bovine growth hormone gene poly(A)-addition si
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3006..3242
                     /note="the human bcl-2 gene splice acceptor (intron 2 -
                     modified exon 3)"
                     /label=the human bcl-2 gene splice acceptor (intron 2 -
                     m(1)
                     /ApEinfo_label=the human bcl-2 gene splice acceptor
                     (intron 2 - m
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3919..4170
                     /note="the bovine growth hormone gene poly(A)-addition
                     signal #2"
                     /label=the bovine growth hormone gene poly(A)-addition
                     si(1)
                     /ApEinfo_label=the bovine growth hormone gene
                     poly(A)-addition si
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     4174..4480
                     /note="the human growth hormone gene poly(A)-addition
                     signal #1"
                     /label=the human growth hormone gene poly(A)-addition sig
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     4495..4808
                     /note="the human growth hormone gene poly(A)-addition
                     signal #2"
                     /label=the human growth hormone gene poly(A)-addition
                     sig(1)
                     /ApEinfo_label=the human growth hormone gene
                     poly(A)-addition sig
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3262..3288
                     /label=HA-tag
                     /ApEinfo_fwdcolor=#ffe912
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4816..4849)
                     /note="loxP"
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3243..3261
                     /label=Bcl-2 exon2
                     /ApEinfo_fwdcolor=#1bd5ff
                     /ApEinfo_revcolor=#46ff92
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(5024..5057)
                     /note="lox5171"
                     /label=lox5171(1)
                     /ApEinfo_label=lox5171
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(5103..5136)
                     /note="FRT site; Flp recognition site"
                     /label=FRT site; Flp recognition site(1)
                     /ApEinfo_label=FRT site; Flp recognition site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5175..5876
                     /note="the mouse RNA polymerase II (the RPB1 subunit) gene
                     promoter"
                     /label=the mouse RNA polymerase II (the RPB1 subunit) gen
                     /ApEinfo_fwdcolor=#0cff35
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     6708..6798
                     /note="a synthetic base sequence for 3' RACE PCR"
                     /label=a synthetic base sequence for 3' RACE PCR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6805..7026
                     /note="the mouse hprt gene splice donor (modified exon 8 -
                     intron 8)"
                     /label=the mouse hprt gene splice donor (modified exon 8 
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(7036..7069)
                     /note="F3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT) site"
                     /label=F3 site; synthetic variant 3 of the yeast
                     flp-reco(1)
                     /ApEinfo_label=F3 site; synthetic variant 3 of the yeast
                     flp-reco
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7079..7166
                     /note="a synthetic base sequence for splinkerette genome
                     PCR"
                     /label=a synthetic base sequence for splinkerette genome 
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7173..7210
                     /note="the CC-in-poly(AT) sequence #14"
                     /label=the CC-in-poly(AT) sequence #14
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7214..7243
                     /note="the vector-ID sequence #14"
                     /label=the vector-ID sequence #14
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7258..7292
                     /note="the Term sequence"
                     /label=the Term sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            3635..3645
                     /gene="EGFP"
                     /label=EGFP
                     /ApEinfo_fwdcolor=#09ff0d
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7293..7467
                     /note="R175 (an essential Tol2-transposon sequence)"
                     /label=R175 (an essential Tol2-transposon sequence)
                     /ApEinfo_fwdcolor=#ff030f
                     /ApEinfo_revcolor=#99abff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2168..2187
                     /label=T3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(7526..7543)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2130..2150
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(7497..7517)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      1080..1762
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      7701..8007
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             7614..7682
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    2102..2124
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2511..2544
                     /label=FRT
                     /ApEinfo_fwdcolor=orchid
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(5103..5136)
                     /label=FRT(1)
                     /ApEinfo_label=FRT
                     /ApEinfo_fwdcolor=orchid
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2756..2789
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4816..4849)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             323..982
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7108..7467
                     /label=Tol2
                     /ApEinfo_fwdcolor=#ff9fd2
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7293..7467
                     /label=Tol2_3'end
                     /ApEinfo_fwdcolor=#ff181e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3262..3280
                     /label=HA-tag(1)
                     /ApEinfo_label=HA-tag
                     /ApEinfo_fwdcolor=#ffdb61
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3243..3261
                     /label=bcl2-5AA
                     /ApEinfo_fwdcolor=#f0a1ff
                     /ApEinfo_revcolor=#faa7ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3393..3415
                     /label=GSP1_GFP/VC_R
                     /ApEinfo_fwdcolor=#20ff64
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            5893..6696
                     /gene="Neo"
                     /label=Neo
                     /ApEinfo_fwdcolor=#ffe511
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7108..7137
                     /label=SPL-2m
                     /ApEinfo_fwdcolor=#ffef13
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7079..7107
                     /label=SPL-1
                     /ApEinfo_fwdcolor=#ffef13
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaGCTAG Ctggcggcgg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tgggcccggc tcgatctccg cggccgcatg atcgattgcc gaagttccta
     2521 ttctctagaa agtataggaa cttcggcatc ggcatcaatt catcatcgga tatttgcatc
     2581 gcaatatcaa tccattgccc ggatggccgg atgatcgatg gcggatgcca tgcgatggat
     2641 cggaaatcgc atccattccg gccgaagttc ctattcttca aatagtatag gaacttcggc
     2701 gaattcggaa tcattggcaa tgccatccat cggatttgat gccatgcaat tccggataac
     2761 ttcgtatagc atacattata cgaagttatc cgaaagctgt ccccaatcct cccaccacga
     2821 cccgccgcac tgcagatacc gcaccgtatt ggcaagtagc ccgtaaacgc ggcgaatcgc
     2881 agccagcata gccaggtcca gccgctcgcc ggggcgctgg cgtttggcca ggcggtcgat
     2941 gtgtctgtcc tccggaaggg cccggataac ttcgtatagt acacattata cgaagttatc
     3001 cgggatctgc tgtatctcta agatggctgg cacgtaaaga gggtctcaat aaatatgcct
     3061 tgaattaatg aaaacagtgg tttctgaatc atggcctcca aagagcattt gccttttccc
     3121 ctctcaccct tctgaaggtg cccaggctgc cctggcagga tgcctctttc tctgtggggt
     3181 ggcattctct gctctctctc taatactctt tctttttctt ccctcctctc ccccaactgc
     3241 agGATGCCTT TGTGGAACTG Ctacccatac gatgttccag attacGCTCT TATGGCCATG
     3301 GAGGCCCGAA TTCGGTCGAC CGAGATCTCT CGAGGTACCC GTCCGGCGTG CAAAATCCCG
     3361 AACGACCTGA AACAGAAAGT CATGAACCAC GAcaagcaga agaacggcat caaggCCAAC
     3421 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
     3481 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
     3541 TCCAAACTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
     3601 ACCGCCGCCG GGATCACTCT CGGcatggac gagctgtaca agtaaagcgc cagcacagtg
     3661 gtcgagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3721 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3781 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3841 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3901 ctgaggcgga aagctagact cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3961 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4021 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4081 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4141 ctctatggct tctgaggcgg aaagcttcag cgatggcttc tagctgcccg ggtggcatcc
     4201 ctgtgacccc tccccagtgc ctcttctggc cctggaagtt gccactccag tgcccaccag
     4261 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     4321 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     4381 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     4441 ctccgcctcc tgggttcaag cgattctcct gcctcagcct tctagcttca gcgatggctt
     4501 ctagctgccc gggtggcatc cctgtgaccc ctccccagtg cctcttctgg ccctggaagt
     4561 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     4621 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     4681 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     4741 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     4801 tcccgagtcg agcggataac ttcgtataat gtatgctata cgaagttatc cgcgccaggt
     4861 cgcagatcgt cggtatggag ccgggggtgg tgacgtgggt ctggaccatc ccggaggtaa
     4921 gttgcagcag ggcgtcccgg cagccggcgg gcgattggtc gtaatccagg ataaagacgt
     4981 gcatggaacg gaggcgtttg gccaagacgt ccaaggccca cggataactt cgtataatgt
     5041 gtactatacg aagttatccg atcgatatcc atcccaaata tgcgatggat ggatcggatg
     5101 ccgaagttcc tatactttct agagaatagg aacttcggca ttgcggccgc aaatagatcc
     5161 atccaatgaa gcttgggctg aacatcgagc gccagggctc cgtaaagcta ctagagcaca
     5221 ggcggtgccc caacgtcctg gggcctctcc actaataacg gctacttcca attgattgga
     5281 cgcgccatct tgcctgcctt atgcatattc agcggtgaac tgaatattca tgaacgaggc
     5341 ccgtcccgtc cctccctcct tccccccacc cccggaaccc gctccggagg acccgaaggg
     5401 ccccgccttc attaccgatg cgtaggacaa accattttcc cgatgtgtgt ggggggatac
     5461 taatgagaga ctttagctga aaaatgagcc tgaactccga agctgagtaa aaatggccta
     5521 actttatcct ccgttctgta agtcctcggt ttgagtgcac gggaaacccg aaaggaggac
     5581 gacaggacca ggaacattct cctcctcctg tcgcgtcaga aagaacaccc aaccagggag
     5641 ccggagccct agcgtcaaca actccgccgc gcgcgctccg tgtaggccgg tgcgggcggc
     5701 cccgtagcgc aagggagggc gggaaaggaa ggggcgggac acaagggcga atctataaag
     5761 ggcgtcactc agccagttct ctcctcagaa gcgccgagag cgcgaccggg acggttggag
     5821 aagaaggtgg ctcccggaag ggggagagac aaactgccgt aacctctgcc gttcaggatc
     5881 cgtgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     5941 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6001 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6061 tccggtgcCC TGAATGAACT GCAGGACGag gcagcgcggc tatcgtggct ggccacgacg
     6121 ggcgttcctt gcgcagctgt gctcgacGTT GTCACTGAAG CGGGAAGgga ctggctgcta
     6181 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6241 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6301 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6361 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6421 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     6481 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6541 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6601 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6661 atcgccttct atcGCCTTCT TGACGAGTTC TTCTGAccAC CGGTgacgcc aTGGCCAATC
     6721 GCATCATGGA TTCCatccga tgcgaatcgc atccatggat gcgccaatcg gattccatgc
     6781 gatgccattt ccgatgcggg atccgaaatt ccagacaagt ttgttgttgg atatgccctc
     6841 gactacaacg agtacttcag gaatttgcag gtaagtaatg cttttttttt tctcgctctc
     6901 atttttcaaa aacccacata aaaattgagg aaagggaaga attgttttct ccttccagca
     6961 cctcgtaatt tgacccgact gatggttccc attagtcaca taaagctgta gtcaagtaca
     7021 gacgtcgaat tcgccgaagt tcctatacta tttgaagaat aggaacttcg gcctcgagCA
     7081 TGCATCATAT CCATCGCAAT CGCATCCCCA TTCAATTCAC ATCGCATATC CGATGCCGAT
     7141 ATGGCATCGG CATTGCCATC CAATCCTGAT CAatccatat atGATGatat atatatGATG
     7201 atatatatat ATTTTTCCAT TGCATCATCC CCGATCATGC CATATCCGAA TGGATCCATG
     7261 CGGATCATCG CATCCATTGC GATAAAGGAT CTTTCTTGCT TTTACTTTTA CTTCCTTAAT
     7321 ACTCAAGTAC AATTTTAATG GAGTACTTTT TTACTTTTAC TCAAGTAAGA TTCTAGCCAG
     7381 ATACTTTTAC TTTTAATTGA GTAAAATTTT CCCTAAGTAC TTGTACTTTC ACTTGAGTAA
     7441 AATTTTTGAG TACTTTTTAC ACCTCTGtca agaaccatat gccggtaccc aattcgccct
     7501 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7561 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7621 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7681 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7741 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7801 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7861 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7921 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7981 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8041 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8101 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtgg 
//
