LOCUS       pNTK\GataD2a\flox\targeting\construct\final        7828 bp    DNA     circular      9-APR-2016
DEFINITION  Complementary copy of JH-1.02  pNTKV1901.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|-11186820|
COMMENT     VNTDBDATE|-11186820|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    complement(1637..1656)
                     /vntifkey="21"
                     /label=pNTKV\1901\seq4\R
     misc_feature    complement(6038..6057)
                     /vntifkey="21"
                     /label=pNTKV\1901\seq1
     misc_feature    4774..4906
                     /vntifkey="21"
                     /label=exon2
     misc_feature    5044..5175
                     /vntifkey="21"
                     /label=exon3
     misc_feature    5366..5455
                     /vntifkey="21"
                     /label=exon4
     misc_feature    complement(4956..4989)
                     /vntifkey="21"
                     /label=Lox
     misc_feature    3025..4598
                     /vntifkey="21"
                     /label=PGK-neo
     misc_feature    complement(2959..2992)
                     /vntifkey="21"
                     /label=Frt
     misc_feature    complement(4635..4668)
                     /vntifkey="21"
                     /label=Frt
     misc_feature    complement(2909..2942)
                     /vntifkey="21"
                     /label=Lox
     misc_feature    complement(3181..3201)
                     /vntifkey="21"
                     /label=pNTKV\1901\seq3
     misc_feature    complement(4488..4511)
                     /vntifkey="21"
                     /label=pNTKV\1901\seq2\R
     misc_feature    1824..1833
                     /vntifkey="21"
                     /label=5'\arm
     misc_feature    complement(5754..5773)
                     /vntifkey="21"
                     /label=3'\arm
     misc_feature    5407..5407
                     /vntifkey="21"
                     /label=SNP
BASE COUNT     1653 a      2132 c      2242 g      1801 t 
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg 
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     1681 ttattgaagc atttatcagg gttattgtct cgtgagcgga tacatatttg aatgtattta 
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcaa 
     1801 acagctatga ccatggcggc cgcacctagt gactcacctc accctgctca aacccaaacc 
     1861 acagagacag atctgcactg tgacttccac ctaactttat cctgtgtcac agtgccatct 
     1921 agtggacata ttctgagctg ctttccacaa gcaagttggt acttgcttgg gtgctgggat 
     1981 acacaccaag gggcagttta ggttgtccac tgtgagcagt actttttcta ggacttggtg 
     2041 ctcctcccac ctccttcctg tgttacagca acagagcaca ctggctttaa ggatttagaa 
     2101 gcaacagatg tgtatgccct ctgataccct tcacaaagag ggaggtctaa ttttggggtg 
     2161 ctcctgtgtg ggttaactga tgtaggctat attatagcca ctggtcttta gccagtgtgg 
     2221 gaagagtggt cagtgttgtg gacctgtgca tctgacagtg tgtaccaccc ctgctacaac 
     2281 tgagctccta gggagactgg ctgtttgctc acagggaaaa gtgaacttcc tacaccacag 
     2341 gatgtgtgtt agaaggttgg tcacagtggt gagtgttcca tgatgtaact tcttctgaga 
     2401 cttgagtgtg cagggttgag ggcagagcca tcacactctg tagacctgag catgaagtca 
     2461 gtgagagtgg ttcttttgag tttgaaaact tgtagccact gcagccactt gttatgtgtg 
     2521 tcctaggtag gacaatgcag cagatggccc caggtcccct tgtgaggtag agagcaaaag 
     2581 ctaacagtac atcattgtgc tttttggtct tctggggggc tgtatgggac tgggttaaga 
     2641 gaggggtggg cttcccattg ggtagttccc aatgtgagac cttggctttg gagtgctcac 
     2701 gtccatccta cccagcactc aggagctgag atagcagacc aagatctgaa gctctgttta 
     2761 cttgtgacag agctctgggg tcgagtagca ctttctgaag catccatgct acacctgagt 
     2821 actcagagat ggagcacacc atctggtgtg tatggcccta atgaccagtg atgataccca 
     2881 tggtgcccac gcccgggtct agccgcggat aacttcgtat aatgtatgct atacgaagtt 
     2941 atgaattcta ggatcgatga agttcctata ctttctagag aataggaact tcggatcccg 
     3001 gcgcgcccta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 
     3061 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 
     3121 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 
     3181 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 
     3241 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 
     3301 gggtaggcct ttggggcagc ggccaatagc agctttggct ccttcgcttt ctgggctcag 
     3361 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 
     3421 gcccgaaggt cctccggaag cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 
     3481 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg gatcggccat 
     3541 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 
     3601 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 
     3661 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 
     3721 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 
     3781 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 
     3841 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 
     3901 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 
     3961 gcgagcacgt actcggatgg aagccggtct tgtcaatcag gatgatctgg acgaagagca 
     4021 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 
     4081 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 
     4141 cttttctgga ttcatcgact gtggccggct gggtgtggcg gatcgctatc aggacatagc 
     4201 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 
     4261 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 
     4321 gttcttctga ggggatcaat tctctagagc tcgctgatca gcctcgactg tgccttctag 
     4381 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 
     4441 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 
     4501 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 
     4561 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 
     4621 gcgcgcccct cgaggaagtt cctatacttt ctagagaata ggaacttcgg taccgtggca 
     4681 agaggaggca cctaggggtg attgggcatc tggggtagtc tggcctgcct ggtactctgc 
     4741 cctgctaagc acctgcttat ctccatttga cagtgacatg aagtctgaga agagacctcc 
     4801 ctcgcctgat gtgattgtgc tctctgacag tgagcagcca tcgagcccaa gggtgaatgg 
     4861 cctgaccaca gtagccttga aagacaccag cactgaggcg ctcttggtga gccttggcag 
     4921 agcctggaag ccccaccacc ccaccttgca agcttataac ttcgtataat gtatgctata 
     4981 cgaagttata gatctagagc ttctgctgag gctttcccca attttgtgct tttgctctcc 
     5041 tagaaaagca gcccagaaga acgagagcgg atgatcaagc agctgaagga ggagctgagg 
     5101 ctagaggaag caaaactggt cttgttgaag aagctgcggc agagtcagat acagaaggaa 
     5161 gccaccgcac agaaggtgcc tgtgctctac tgccccagac cccagagagg ctggcccagg 
     5221 gcagaggctc gacccacggg cgggatggca ggcagtgtat ggcgttaggg agcaagcaga 
     5281 aagtaattcg gggtgggggg gtgcgtgcgt gcgtgcgtgt aactttacac accaactcct 
     5341 tttgatgtct cttgctatct tatagcctac agcttcctca gggagcactg tgaccacccc 
     5401 tccccccctc gtgcggggta cccagaacat tcctgctggc aagacctcac ttcaggttcg 
     5461 tgcgcccttc tgcttgttgc cctgcaccac ctccgtttat agtcaggctc caaacactgc 
     5521 ctgctcctcc caagtaaaga agcatggggc tcccacagac taaaggacca gagtgtcaca 
     5581 tgcgccccaa attacaccac caaactaggc tactatgaat gtcgcagact ggtcagtggt 
     5641 gattgagcag taagactgaa agattggcta gagtgggtcg ctccgctcag gcctctgccc 
     5701 acagactctg gggcctctgt cacactctcg tattaccttc ctctgagttc ccatggctcc 
     5761 tgctagagta atgcctagag ttaacactgg ccgtcgtttt accggtccgt agtcaggttt 
     5821 agttggtccg gcggcgccag aaatccgcgc ggtggttttt gggggtcggg ggtgtttggc 
     5881 agccacagac gcccggtgtt cgtgtcgcgc cagtacatgc ggtccatgcc caggccatcc 
     5941 aaaaaccatg ggtctgtctg ctcagtccag tcgtggactg accccacgca acgcccaaaa 
     6001 taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct aacccacggg 
     6061 gcccgtggct atggcaggcc tgccgcccga cgttggctgc gagccctggg ccttcacccg 
     6121 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc 
     6181 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg 
     6241 acccaacacc cgtgcgtttt attctgtctt tttattgccg acatagcgcg ggttccttcc 
     6301 ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccgtgcaa acgtgcgcgc 
     6361 caggtcgcag atcgtcggta tggagcctgg ggtggtgacg tgggtctgga tcatcccgga 
     6421 ggtaagttgc agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa 
     6481 gacgtgcatg ggacggaggc gtttggccaa gacgtccaag gcccaggcaa acacgttgta 
     6541 caggtcgccg ttgggggcca gcaactcggg ggcccgaaac agggtaaata acgtgtcccc 
     6601 gatatggggt cgtgggcccg cgttgctctg gggctcggca ccctggggcg gcacggccgt 
     6661 ccccgaaagc tgtccccaat cctcccgcca cgacccgccg ccctgcagat accgcaccgt 
     6721 attggcaagc agcccgtaaa cgcggcgaat cgcggtcagc atagccaggt caagccgctc 
     6781 gccggggcgc tggcgtttgg ccaggcggtc gatgtgtctg tcctccggaa gggcccccaa 
     6841 cacgatgttt gtgccgggca aggtcggcgg gatgagggcc acgaacgcca gcacggcctg 
     6901 gggggtcatg ctgcccataa ggtatcgcgc ggccgggtag cacaggaggg cggcgatggg 
     6961 atggcggtcg aagatgaggg tgagggccgg gggcggggca tgtgagctcc cagcctcccc 
     7021 cccgatatga ggagccagaa cggcgtcggt cacggtataa ggcatgccca ttgttatctg 
     7081 ggcgcttgtc attaccaccg ccgcgtcccc ggccgatatc tcaccctggt caaggcggtg 
     7141 ttgtgtggtg tagatgttcg cgattgtctc ggaagccccc agcacccgcc agtaagtcat 
     7201 cggctcgggt acgtagacga tatcgtcgcg cgaacccagg gccaccagca gttgcgtggt 
     7261 ggtggttttc cccatcccgt ggggaccgtc tatataaacc cgcagtagcg tgggcatttt 
     7321 ctgctccggg cggacttccg tggcttcttg ctgccggcga gggcgcaacg ccgtacgtcg 
     7381 gttgctatgg ccgcgagaac gcgcagcctg gtcgaacgca gacgcgtgct gatggccggg 
     7441 gtacgaagcc atacgcgctt ctacaaggcg ctggccgaag aggtgcggga gtttcacgcc 
     7501 accaagatgt gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg tcgctcggtg 
     7561 ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg ccgccccgac 
     7621 tgcatctgcg tgttccaatt cgccaatgac aagacgctgg gcggggtttg ctcgacattg 
     7681 ggtggaaaca ttccaggcct gggtggagag gctttttgct tcctcttgca aaaccacact 
     7741 gctcgacatt gggtggaaac attccaggcc tgggtggaga ggctttttgc ttcctcttga 
     7801 aaaccacact gctcgactct acggtccg 
//
