LOCUS       Exported                3852 bp ds-DNA     circular SYN 18-APR-2018
DEFINITION  Ligation of pFA6a-natN* into pUG6*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 18, 2018 from SnapGene Viewer 4.1.4
            http://www.snapgene.com
COMMENT     Vector molecule:  pUG6*
               Fragment ends: SacI and BglII
               Fragment size: 2562
            Insert molecule:  pFA6a-natN*
               Fragment ends: BglII and SacI
               Fragment size: 1290
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    12..31
                     /label=FW
     primer_bind     12..31
                     /label=rev
     misc_feature    53..86
                     /label=LoxP
     misc_feature    53..86
                     /label=LoxP site
     misc_feature    485..1060
                     /label=nat
     misc_feature    1403..1436
                     /label=LoxP site
     misc_feature    1403..1436
                     /label=LoxP
     misc_feature    1447..1468
                     /label=RV
     primer_bind     complement(1447..1468)
                     /label=fwd
     misc_feature    complement(2525..3385)
                     /label=AmpR
                     /note="ampicillin resistance protein"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc
       61 gtataatgta tgctatacga agttattagg tctagagatc tgtttagctt gcctcgtccc
      121 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
      181 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
      241 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
      301 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
      361 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
      421 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
      481 aaccatgggt accactcttg acgacacggc ttaccggtac cgcaccagtg tcccggggga
      541 cgccgaggcc atcgaggcac tggatgggtc cttcaccacc gacaccgtct tccgcgtcac
      601 cgccaccggg gacggcttca ccctgcggga ggtgccggtg gacccgcccc tgaccaaggt
      661 gttccccgac gacgaatcgg acgacgaatc ggacgacggg gaggacggcg acccggactc
      721 ccggacgttc gtcgcgtacg gggacgacgg cgacctggcg ggcttcgtgg tcatctcgta
      781 ctcggcgtgg aaccgccggc tgaccgtcga ggacatcgag gtcgccccgg agcaccgggg
      841 gcacggggtc gggcgcgcgt tgatggggct cgcgacggag ttcgccggcg agcggggcgc
      901 cgggcacctc tggctggagg tcaccaacgt caacgcaccg gcgatccacg cgtaccggcg
      961 gatggggttc accctctgcg gcctggacac cgccctgtac gacggcaccg cctcggacgg
     1021 cgagcggcag gcgctctaca tgagcatgcc ctgcccctaa tctcgaggcg aatttcttat
     1081 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1141 gtgactctta ggttttaaaa cgaaaattct tgttcttgag taactctttc ctgtaggtca
     1201 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     1261 cgagcaaatg cctgcaaatc gctccccatt tcacccaatt gtagatatgc taactccagc
     1321 aatgagttga tgaatctcgg tgtgtatttt atgtcctcag aggacaacac ctgttgtaat
     1381 cgagctctcg agaaccctta atataacttc gtataatgta tgctatacga agttattagg
     1441 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     1501 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     1561 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1621 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1681 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1741 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1801 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1861 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1921 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1981 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2041 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2101 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2161 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2221 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2281 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2341 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2401 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2461 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2521 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2581 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2641 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2701 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2761 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2821 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2881 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2941 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3001 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3061 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3121 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3181 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3241 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3301 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3361 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3421 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3481 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3541 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     3601 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3661 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3721 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     3781 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     3841 ggtgacacta ta
//
