LOCUS Exported 8680 bp ds-DNA circular SYN 15-APR-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8680) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..8680 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" misc_feature 417..1437 CDS 1446..1994 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(1666..1685) misc_feature 1666..1685 CDS 1995..2997 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 2998..3110 /label=Flag-His /note="Flag-His" 3'UTR 3124..3218 /label=polyA /note="polyA" misc_feature complement(3286..3305) rep_origin 3515..5381 /label=OriP /note="OriP" CDS 5613..6473 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 6618..7291 /label=pUC origin /note="pUC origin" promoter 7921..8509 /note="CMV promoter" TATA_signal 8474..8479 /note="TATA box" misc_feature 8513..8532 /label=TPL1 /note="TPL1" misc_feature 8550..8680 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccgag 421 gaattcgaga agatcaagta ccctcaggac gtgaccaaga ttaagtacta cgacaacagc 481 cacaagatcg acagcaagta cctgtacaag cacgtggcca agggcatgct gcaggacctg 541 atcgtgaccg tgcagatcga caaggacaga gaggtggaca agatcctgtt cctgcccaag 601 aaaaagacct acatgaagat catcggcgat tggagcgact accccaaaga gaagaacaac 661 gagatctgcc tggccttcgt gctgcagaag tactttaaga aagagaaaga aaaaaagtgc 721 accaaggatt tcttcaccag cggcaacatc tacaacgtgt gctacaacga caacaactac 781 gccatcaaga acctgtacct ctacatcgag atcagccaga agtacctcga gaagctgttc 841 agcagctaca gcatccccgt gatcgtgaac accgagaaca acatcatcta cttcgccaac 901 gacaagatga agtccaaaga ggaaaagccc atcctggacc tgcacatctg ctacaagagc 961 aactacgaga cacccaccta cctgggcaag ctgatcttca actcctaccc catcaacatc 1021 aaccggaaca aggaccagtt cgacattatc tacaccaaga gctttctgac caacaactgg 1081 gactacaacc tggccatcaa gggcaactac atcaccagct acaaccgggt ggtgttcgtg 1141 cggatcccca gagacagccc tcccaacaaa atgaagtgcc cacccttctg ggattacagc 1201 gtgctgggca gcggccctct gcacaagatc aagaagaaaa acaagaacaa tatcattcag 1261 aacatggtgc agtacagctt cagcaacaag aatatccaga actactacat cccattcctc 1321 aagaaggacg agctgcagac caacgaggcc tacatcatct gcctgtacag cgacgagaac 1381 gacttcgagg gcatgcagat catcaacgcc cacttctaca tcaacaccgc cgacagcggc 1441 gcgccgtcga cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc 1501 ccactcaacc ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct 1561 ccttcttccc agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag 1621 tctactagca accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc 1681 caggtctccc ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata 1741 ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg 1801 acctgtgagg tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat 1861 caggaggcca gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg 1921 gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt 1981 ttatccaaag ggttgaattc cggcggcggc tccggaggcg gtaccggtgg cggcgaccta 2041 gccccacaga tgcttcgaga actccaggag actaatgcgg cgctgcaaga cgtgagagag 2101 ctcttgcgac agcaggtcaa ggagatcacc ttcctgaaga atacggtgat ggaatgtgac 2161 gcctgcggtc tagacagaaa cctgcctcca ctagcacctc tgggcccaca cccagaaacg 2221 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 2281 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 2341 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 2401 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 2461 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 2521 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 2581 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 2641 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 2701 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 2761 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 2821 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 2881 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 2941 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggggc 3001 cctgactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac 3061 gatgacaagg gccctcacca ccaccatcac cactaattaa ttaaggatcc cccgacctcg 3121 acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt 3181 ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct 3241 ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc 3301 cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg 3361 ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt 3421 gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca 3481 gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag 3541 ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc 3601 tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc 3661 attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc 3721 gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg 3781 aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca 3841 ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc 3901 attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc 3961 ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca 4021 gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt 4081 tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc 4141 ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc 4201 catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg 4261 ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg 4321 gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga 4381 cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc 4441 cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag 4501 tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg 4561 taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa 4621 ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg 4681 cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg 4741 ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt 4801 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca 4861 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta 4921 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca 4981 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca 5041 tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca 5101 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg 5161 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg 5221 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg 5281 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg 5341 ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag 5401 aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 5461 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 5821 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg 5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 6121 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca 6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 6301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 6781 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 7261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 7321 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 7381 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 7441 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 7501 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 7561 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 7621 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt 7681 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg 7741 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg 7801 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa 7861 tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac 7921 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 7981 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 8041 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 8101 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 8161 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 8221 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 8281 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg 8341 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 8401 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt 8461 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcc tcactctctt 8521 ccgcatcgct gtctgcgagg gccagctgtt gggctcgcgg ttgaggacaa actcttcgcg 8581 gtctttccag tactcttgga tcggaaaccc gtcggcctcc gaacggtact ccgccaccga 8641 gggacctgag cgagtccgca tcgaccggat cggaaaacct //