LOCUS Exported 8938 bp ds-DNA circular SYN 04-AUG-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8938) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..8938 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..1695 /codon_start=1 /label=PF3D7_1463900 /note="residues deleted (2014-08-04)" /translation="QREQLLHQDPAEPGKEDRERSLPVREAEQPDPVRGQRRLQQPGQA EVPAELPEED*QHHPQEAERGKQAQEVPAGHQERQQHLPRGQQPSGLREERARGKRAGK NLHEQRDQQDQRAEGQLLRAQQEHQQQVHRGPDQQRPAVQQG*DQEPVQQSPPGKIHPE ARARERARGQERRQKRAQE*A*ERTQK*TRPRARA*QQEL*GQPSQERVPQHQLRQHPE QHRLLQENRPGQELLRLQQQRGPEPEHEGTEKHAEPPRGKREEQHDPGQQPGGRESEQS PV*QQLPQPEERQPEAGHQGQQRRADLQQCQQQYRGQQSHQVLHLQGHREKKEQAERHQ *GRVQRHQKQEPAPPAEQHQRNLRRGRPREEYQELPGHQEQHPRGLREPEADHQELQLR ELRPRAVLRG*EGQRNAVEV*EEEKLR" CDS 1704..2252 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(1924..1943) misc_feature 1924..1943 CDS 2253..3255 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 3256..3368 /label=Flag-His /note="Flag-His" 3'UTR 3382..3476 /label=polyA /note="polyA" misc_feature complement(3544..3563) rep_origin 3773..5639 /label=OriP /note="OriP" CDS 5871..6731 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 6876..7549 /label=pUC origin /note="pUC origin" promoter 8179..8767 /note="CMV promoter" TATA_signal 8732..8737 /note="TATA box" misc_feature 8771..8790 /label=TPL1 /note="TPL1" misc_feature 8808..8938 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaac 421 gagaacaact tcttcatcaa gatcctgcgg agccaggaaa agaagatcga gaacgaagtc 481 tccctgtacg agaagctgaa caaccggatc ctgttcgagg gcagcgacga cttcagcaac 541 ctggacaagc tgaagtccct gctgagcttc cagaagaaga ttgacaacac catccgcaag 601 aagcagaaag aggaaaacaa gctcaagaag ttccagcagg ccatcaagaa cggcaacaac 661 atctaccccg tgggcaacaa ccctcagggc ttcgagaaga aagagcgcga ggaaaaagag 721 caggaaaaaa cctacatgaa caacgtgatc aacaagatca acgagcagaa gggcaactac 781 tacgagccca acaagaacat caacaacaag tacaccgagg acctgaccag caacggcctg 841 ctgtacaaca aggataagat caagaaccag ttcaacaatc cccaccagga aaaattcatc 901 cggaagcacg agcacgagag agagcacgag ggcaagaacg aaggcaaaaa cgagcacaag 961 aatgagcata agaacgaaca caaaaatgaa cacgtccacg tgcacgagca taacagcaag 1021 aactatgagg gcaaccctcc caagaacgag taccccaaca tcaacttcgc caacatcctg 1081 aacagcatcg acttctacaa gaaaatcgac ctggtcaaga actacttcgg ctacaacaac 1141 aacgaggccc tgaaccagaa catgaaggaa cagaaaaaca tgcagaacca ccacgtggaa 1201 aacgagaaga acaacatgat ccaggccaac aaccaggtgg acgagaatca gaacaatcac 1261 cagtataaca acaacttcca caaccagaag aacgacaacc tgaagcagga caccaaggac 1321 aacagcgccg agcagaccta caacaatgcc aacaacaata tcgaggacaa caatcacatc 1381 aagttcttca tctccaagga catcgagaaa aaaaagaaca agcagaacga catcaatgag 1441 gccgtgtaca acgccatcaa aaacaagaac ctgcacctcc ggcggaacaa catcaaagaa 1501 atcttcgacg tggacgacct cgtgaagaat atcaagagct tcctgggcat caagagcaac 1561 atccacgagg ccttcgagaa ccagaagctg atcatcaaga attgcaacta cgagagcttc 1621 ggccccgagc tgtgctccgt ggatgagaag gccaaagaaa tgctgtggaa gtatgagaag 1681 aagaaaaact ccgccggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag 1741 tcagcggagt tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga 1801 tggaaggcag agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa 1861 aaggtgtctg tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca 1921 ctcacccttc agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg 1981 actctggaca gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag 2041 cccgacagca acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg 2101 atcttgaagc aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg 2161 caggcccctg aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg 2221 gactccaaga tccaggtttt atccaaaggg ttgaattccg gcggcggctc cggaggcggt 2281 accggtggcg gcgacctagc cccacagatg cttcgagaac tccaggagac taatgcggcg 2341 ctgcaagacg tgagagagct cttgcgacag caggtcaagg agatcacctt cctgaagaat 2401 acggtgatgg aatgtgacgc ctgcggtcta gacagaaacc tgcctccact agcacctctg 2461 ggcccacacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 2521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 2581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 2641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 2701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 2761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 2821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 2881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 2941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 3001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 3061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 3121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 3181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 3241 ctgattaagc attggggccc tgactacaaa gaccatgacg gtgattataa agatcatgac 3301 atcgattaca aggatgacga tgacaagggc cctcaccacc accatcacca ctaattaatt 3361 aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg 3421 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc 3481 gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga actaaacctg 3541 actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg 3601 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg 3661 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt 3721 ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg 3781 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc 3841 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa 3901 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac 3961 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag 4021 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag 4081 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt 4141 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc 4201 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata 4261 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga 4321 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat 4381 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata 4441 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca 4501 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta 4561 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac 4621 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc 4681 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt 4741 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg 4801 gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca 4861 aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg 4921 cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc 4981 tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg 5041 gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta 5101 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta 5161 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta 5221 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta 5281 atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata tgctatccta 5341 atttatatct gggtagcata tactacccaa atatctggat agcatatgct atcctaatct 5401 atatctgggt agcatatgct atcctaatct atatctgggt agcataggct atcctaatct 5461 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt 5521 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct 5581 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatga 5641 taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt 5701 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 5761 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 5821 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 5881 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 5941 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 6001 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 6061 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg 6121 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 6181 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 6241 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 6301 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 6361 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa 6421 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 6481 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 6541 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 6601 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 6661 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 6721 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 6781 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 6841 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 6901 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 6961 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 7021 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 7081 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 7141 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 7201 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 7261 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 7321 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 7381 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 7441 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 7501 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 7561 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 7621 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 7681 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 7741 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 7801 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 7861 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg 7921 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt 7981 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt 8041 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac 8101 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg 8161 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 8221 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 8281 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 8341 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 8401 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc 8461 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt 8521 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata 8581 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 8641 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca 8701 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 8761 tcagatcctc actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt 8821 gaggacaaac tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga 8881 acggtactcc gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacct //