LOCUS Exported 8095 bp ds-DNA circular SYN 12-JUN-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8095) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..8095 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..852 /codon_start=1 /label=PF3D7_0713700 /note="residues deleted (2014-06-12)" /translation="RHHHRGQQQGHPGLRPQQRAEPGRERVQHLPGHQDQQRRYQDRVQ QHLQQQQSDHRRHL*QHEGTDQGQKPEVPGRAGHRGIREQRRCVQLRHLRVHRERPARY HPAGLQEAPQLHQLHHHL*RERAVRQHRGRQGQQECVPHQP" CDS 861..1409 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(1081..1100) misc_feature 1081..1100 CDS 1410..2412 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 2413..2525 /label=Flag-His /note="Flag-His" 3'UTR 2539..2633 /label=polyA /note="polyA" misc_feature complement(2701..2720) rep_origin 2930..4796 /label=OriP /note="OriP" CDS 5028..5888 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 6033..6706 /label=pUC origin /note="pUC origin" promoter 7336..7924 /note="CMV promoter" TATA_signal 7889..7894 /note="TATA box" misc_feature 7928..7947 /label=TPL1 /note="TPL1" misc_feature 7965..8095 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccgac 421 atcatcatcg tggacagcaa caaggacatc cagggcttcg accccaacag cgtgctgaac 481 ctggccgaga acgtgttcaa catcttcctg gacaccaaga ccaacaacgc cgatatcaag 541 accgtgtaca gcagcatctt cagcaacagc aaagtgacca tcgacggcac ctgtagcagc 601 atgaaggaac tgatcaaggg cagaagcctg aagtacctgg acgagctggg caccgaggaa 661 tacgtgaaca gcgacgatgt gttcaacttc gccatcttcg tgtacaccgt gaacggccag 721 cccgatatca tccagcaggt ctacaagaag caccccaact acatcagcta catcatcacc 781 tgtaaagaga gcgagctgtt cgacaacatc gaggacgcca aggacaacaa gaatgtgttc 841 cacaccaacc ccggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca 901 gcggagttct ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg 961 aaggcagaga aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag 1021 gtgtctgtgc agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc 1081 acccttcaga taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact 1141 ctggacagag ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc 1201 gacagcaaca ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc 1261 ttgaagcagg agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag 1321 gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac 1381 tccaagatcc aggttttatc caaagggttg aattccggcg gcggctccgg aggcggtacc 1441 ggtggcggcg acctagcccc acagatgctt cgagaactcc aggagactaa tgcggcgctg 1501 caagacgtga gagagctctt gcgacagcag gtcaaggaga tcaccttcct gaagaatacg 1561 gtgatggaat gtgacgcctg cggtctagac agaaacctgc ctccactagc acctctgggc 1621 ccacacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2401 attaagcatt ggggccctga ctacaaagac catgacggtg attataaaga tcatgacatc 2461 gattacaagg atgacgatga caagggccct caccaccacc atcaccacta attaattaag 2521 gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt 2581 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag 2641 atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact aaacctgact 2701 acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac 2761 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt 2821 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc 2881 tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt 2941 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg 3001 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg 3061 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg 3121 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat 3181 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa 3241 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca 3301 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt 3361 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact 3421 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc 3481 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata 3541 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct 3601 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac 3661 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga 3721 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag 3781 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa 3841 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt 3901 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac 3961 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac 4021 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg 4081 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga 4141 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct 4201 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc 4261 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc 4321 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc 4381 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc 4441 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt 4501 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata 4561 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata 4621 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata 4681 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata 4741 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgataa 4801 gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc ctatttttat 4861 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 4921 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4981 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 5041 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 5101 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 5161 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 5221 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 5281 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5341 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5401 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5461 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5521 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg 5581 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5641 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5701 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 5761 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5821 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5881 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 6781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 6841 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 6901 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 6961 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 7021 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg 7081 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct 7141 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc 7201 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt 7261 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg 7321 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 7381 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 7441 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 7501 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 7561 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 7621 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt 7681 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg 7741 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 7801 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat 7861 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca 7921 gatcctcact ctcttccgca tcgctgtctg cgagggccag ctgttgggct cgcggttgag 7981 gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg cctccgaacg 8041 gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa aacct //