LOCUS       Exported                8461 bp ds-DNA     circular SYN 15-APR-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled1 - Sequence
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 19, 2018 from SnapGene Viewer 4.1.8
            http://www.snapgene.com
COMMENT     New NA Sequence
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..172
                     /label=TPL2/3
                     /note="TPL2/3"
     misc_feature    154..156
                     /label=SD
                     /note="SD"
     promoter        181..331
                     /note="MLP"
     misc_feature    339..341
                     /label=SA
                     /note="SA"
     CDS             352..408
                     /codon_start=1
                     /label=Kozak+leader Vkappa 7-33
                     /note="Kozak+leader Vkappa 7-33"
                     /note="residues deleted (2012-01-10)"
                     /translation="ATMEFQTQVLMSLLLCMSG"
     CDS             409..416
                     /codon_start=1
                     /label=Kozak+leader Vkappa 7-33
                     /note="Kozak+leader Vkappa 7-33"
                     /translation="AA"
     misc_feature    417..1218
     CDS             1227..1775
                     /codon_start=1
                     /label=AscI CD4d3+4
                     /note="AscI CD4d3+4"
                     /note="residues deleted (2006-03-14)"
                     /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD
                     HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE
                     PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS
                     TE*R*RGQDGLQDPGFIQRV"
     misc_feature    complement(1447..1466)
     misc_feature    1447..1466
     CDS             1776..2778
                     /codon_start=1
                     /label=L1 - COMP - L2 - Blac
                     /note="L1 - COMP - L2 - Blac"
                     /note="residues deleted (2010-08-05)"
                     /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH
                     LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*
                     EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH
                     DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR
                     RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I
                     WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL"
     misc_feature    2779..2891
                     /label=Flag-His
                     /note="Flag-His"
     3'UTR           2905..2999
                     /label=polyA
                     /note="polyA"
     misc_feature    complement(3067..3086)
     rep_origin      3296..5162
                     /label=OriP
                     /note="OriP"
     CDS             5394..6254
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    6399..7072
                     /label=pUC origin
                     /note="pUC origin"
     promoter        7702..8290
                     /note="CMV promoter"
     TATA_signal     8255..8260
                     /note="TATA box"
     misc_feature    8294..8313
                     /label=TPL1
                     /note="TPL1"
     misc_feature    8331..8461
                     /label=TPL2/3
                     /note="TPL2/3"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaga
      421 atgaacgtgg tgtcccacaa cagcatgaag aaagtgggcg aggaaggcaa ggacgagaag
      481 atcatcagct gcgtggtgga atacgtgatc aagggcgacc tgagcatcga cgacggcgat
      541 ttctgcgaca gcaacagcat ctgcagcaac aaccccggcc tgtgcgacaa cggccaggtg
      601 gtggtgcccg gcgactactg ctccaactac tacgacatca ccctggtggt ggaagagtcc
      661 agcttcgtgc agaaggacta ctggctgaag ggcaccatcc cctttctgga atccatggtg
      721 ttcaacagca acgtggccaa ggacaaggcc cacatgagcg tggtgctgtt cgccggcaag
      781 cagcgggtgg tggtcccctt caccgacgag agcagccagg acaagaacat gctgctggaa
      841 aagatcaact ccatcgacga gggaatcgcc accagcagcg acaccctgta cgtgaacgcc
      901 ctgaagtacg ccatcgagaa cgtgatcttc ggcgacggca cccggaagga cgcccctaag
      961 gtggccgtgc tgttctacta cggcttcgac tacggcgcca acaagaatat gatccccgac
     1021 gtggtgcaga cctacaaaga acagaacgtg aagctgatca tcgtggggat cgccctgggc
     1081 atccgggaca acgcctacct gctggccgac tgcagcatcg gcgacgagaa ctgtgtgaat
     1141 gtggtgttta agccctggga cttcgtgatc cccgctgccg ccgacgtgaa ccagaagatc
     1201 tgcgataaga acaaggccgg cgcgccgtcg acctccatca cggcctataa gagtgagggg
     1261 gagtcagcgg agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg
     1321 agatggaagg cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac
     1381 caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc
     1441 ccactcaccc ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc
     1501 ctgactctgg acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact
     1561 cagcccgaca gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga
     1621 ctgatcttga agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa
     1681 gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag
     1741 atggactcca agatccaggt tttatccaaa gggttgaatt ccggcggcgg ctccggaggc
     1801 ggtaccggtg gcggcgacct agccccacag atgcttcgag aactccagga gactaatgcg
     1861 gcgctgcaag acgtgagaga gctcttgcga cagcaggtca aggagatcac cttcctgaag
     1921 aatacggtga tggaatgtga cgcctgcggt ctagacagaa acctgcctcc actagcacct
     1981 ctgggcccac acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2041 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2101 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2161 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2221 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2281 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2341 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2401 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2461 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2521 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2581 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2641 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2701 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2761 tcactgatta agcattgggg ccctgactac aaagaccatg acggtgatta taaagatcat
     2821 gacatcgatt acaaggatga cgatgacaag ggccctcacc accaccatca ccactaatta
     2881 attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata
     2941 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg
     3001 gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac
     3061 ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt
     3121 tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa
     3181 aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg
     3241 agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt
     3301 atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg
     3361 cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga
     3421 taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta
     3481 aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa
     3541 tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct
     3601 aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg
     3661 attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg
     3721 ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga
     3781 ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg
     3841 tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc
     3901 aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga
     3961 atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc
     4021 tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta
     4081 ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt
     4141 aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc
     4201 cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact
     4261 cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt
     4321 ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg
     4381 tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt
     4441 gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc
     4501 gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg
     4561 tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc
     4621 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4681 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     4741 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4801 ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc
     4861 ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     4921 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     4981 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     5041 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     5101 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca
     5161 tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg atacgcctat
     5221 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     5641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     5941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7201 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7261 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7321 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7381 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag
     7441 aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
     7501 attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca
     7561 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca
     7621 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
     7681 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     7741 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     7801 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     7861 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     7921 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
     7981 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
     8041 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
     8101 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     8161 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
     8221 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     8281 ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tgggctcgcg
     8341 gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc cgtcggcctc
     8401 cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga tcggaaaacc
     8461 t
//
