LOCUS       Exported                8173 bp ds-DNA     circular SYN 29-JUL-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled1 - Sequence
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 19, 2018 from SnapGene Viewer 4.1.8
            http://www.snapgene.com
COMMENT     New NA Sequence
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..172
                     /label=TPL2/3
                     /note="TPL2/3"
     misc_feature    154..156
                     /label=SD
                     /note="SD"
     promoter        181..331
                     /note="MLP"
     misc_feature    339..341
                     /label=SA
                     /note="SA"
     CDS             352..408
                     /codon_start=1
                     /label=Kozak+leader Vkappa 7-33
                     /note="Kozak+leader Vkappa 7-33"
                     /note="residues deleted (2012-01-10)"
                     /translation="ATMEFQTQVLMSLLLCMSG"
     misc_feature    409..938
     CDS             939..1487
                     /codon_start=1
                     /label=AscI CD4d3+4
                     /note="AscI CD4d3+4"
                     /note="residues deleted (2006-03-14)"
                     /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD
                     HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE
                     PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS
                     TE*R*RGQDGLQDPGFIQRV"
     misc_feature    complement(1159..1178)
     misc_feature    1159..1178
     CDS             1488..2490
                     /codon_start=1
                     /label=L1 - COMP - L2 - Blac
                     /note="L1 - COMP - L2 - Blac"
                     /note="residues deleted (2010-08-05)"
                     /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH
                     LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*
                     EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH
                     DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR
                     RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I
                     WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL"
     misc_feature    2491..2603
                     /label=Flag-His
                     /note="Flag-His"
     3'UTR           2617..2711
                     /label=polyA
                     /note="polyA"
     misc_feature    complement(2779..2798)
     rep_origin      3008..4874
                     /label=OriP
                     /note="OriP"
     CDS             5106..5966
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    6111..6784
                     /label=pUC origin
                     /note="pUC origin"
     promoter        7414..8002
                     /note="CMV promoter"
     TATA_signal     7967..7972
                     /note="TATA box"
     misc_feature    8006..8025
                     /label=TPL1
                     /note="TPL1"
     misc_feature    8043..8173
                     /label=TPL2/3
                     /note="TPL2/3"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaaa
      421 gtgaccgtgg acaccgtgtg caagcggggc ttcctgatcc agatgagcgg ccacctggaa
      481 tgcaagtgcg agaacgacct ggtgctggtc aacgaggaaa cctgcgaaga gaaggtgctg
      541 aagtgcgacg agaaaaccgt gaacaagccc tgcggcgact tcagcaagtg catcaagatc
      601 gacggcaacc ccgtgtccta cgcctgcaag tgcaacctgg gctacgacat ggtcaacaac
      661 gtgtgcatcc ccaacgagtg caagaacgtg gcctgcggca acggcaagtg tatcctggac
      721 accagcaacc ctgtgaaaac cggcgtgtgc tcctgcaaca tcggcaaggt gcccaacgtg
      781 caggaccaga acaagtgcag caaggacggc gagacaaagt gcagcctgaa gtgcctgaaa
      841 gagaatgagg cctgcaaggc cgtggacggc atctacaagt gcgactgcaa ggatggcttc
      901 atcatcgaca acgaggccag catctgcacc ggcgcgccgt cgacctccat cacggcctat
      961 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     1021 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     1081 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     1141 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     1201 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     1261 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     1321 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     1381 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     1441 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcggc
     1501 ggctccggag gcggtaccgg tggcggcgac ctagccccac agatgcttcg agaactccag
     1561 gagactaatg cggcgctgca agacgtgaga gagctcttgc gacagcaggt caaggagatc
     1621 accttcctga agaatacggt gatggaatgt gacgcctgcg gtctagacag aaacctgcct
     1681 ccactagcac ctctgggccc acacccagaa acgctggtga aagtaaaaga tgctgaagat
     1741 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1801 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1861 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1921 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1981 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2041 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2101 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2161 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2221 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2281 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2341 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2401 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2461 gagataggtg cctcactgat taagcattgg ggccctgact acaaagacca tgacggtgat
     2521 tataaagatc atgacatcga ttacaaggat gacgatgaca agggccctca ccaccaccat
     2581 caccactaat taattaagga tcccccgacc tcgacctctg gctaataaag gaaatttatt
     2641 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     2701 gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat ccccgccccg
     2761 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     2821 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     2881 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     2941 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     3001 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     3061 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     3121 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     3181 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     3241 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     3301 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     3361 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     3421 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     3481 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     3541 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     3601 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     3661 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     3721 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     3781 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     3841 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     3901 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     3961 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     4021 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     4081 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     4141 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     4201 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     4261 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     4321 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4381 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4441 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4501 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     4561 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     4621 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     4681 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4741 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4801 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     4861 atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga aagggcctcg
     4921 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5341 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5641 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6841 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6901 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6961 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7021 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7081 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7141 agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
     7201 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
     7261 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
     7321 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     7381 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     7441 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     7501 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     7561 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     7621 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     7681 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     7741 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     7801 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     7861 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     7921 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     7981 tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg agggccagct
     8041 gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa
     8101 cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc gcatcgaccg
     8161 gatcggaaaa cct
//
