LOCUS       Exported                9796 bp ds-DNA     circular SYN 15-APR-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled1 - Sequence
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 19, 2018 from SnapGene Viewer 4.1.8
            http://www.snapgene.com
COMMENT     New NA Sequence
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..172
                     /label=TPL2/3
                     /note="TPL2/3"
     misc_feature    154..156
                     /label=SD
                     /note="SD"
     promoter        181..331
                     /note="MLP"
     misc_feature    339..341
                     /label=SA
                     /note="SA"
     CDS             352..408
                     /codon_start=1
                     /label=Kozak+leader Vkappa 7-33
                     /note="Kozak+leader Vkappa 7-33"
                     /note="residues deleted (2012-01-10)"
                     /translation="ATMEFQTQVLMSLLLCMSG"
     misc_feature    417..2553
     CDS             2562..3110
                     /codon_start=1
                     /label=AscI CD4d3+4
                     /note="AscI CD4d3+4"
                     /note="residues deleted (2006-03-14)"
                     /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD
                     HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE
                     PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS
                     TE*R*RGQDGLQDPGFIQRV"
     misc_feature    complement(2782..2801)
     misc_feature    2782..2801
     CDS             3111..4113
                     /codon_start=1
                     /label=L1 - COMP - L2 - Blac
                     /note="L1 - COMP - L2 - Blac"
                     /note="residues deleted (2010-08-05)"
                     /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH
                     LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*
                     EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH
                     DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR
                     RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I
                     WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL"
     misc_feature    4114..4226
                     /label=Flag-His
                     /note="Flag-His"
     3'UTR           4240..4334
                     /label=polyA
                     /note="polyA"
     misc_feature    complement(4402..4421)
     rep_origin      4631..6497
                     /label=OriP
                     /note="OriP"
     CDS             6729..7589
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    7734..8407
                     /label=pUC origin
                     /note="pUC origin"
     promoter        9037..9625
                     /note="CMV promoter"
     TATA_signal     9590..9595
                     /note="TATA box"
     misc_feature    9629..9648
                     /label=TPL1
                     /note="TPL1"
     misc_feature    9666..9796
                     /label=TPL2/3
                     /note="TPL2/3"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccttc
      421 tacttcgagc agaccgagga actgctgatc aagaacgccc acagcgacat ctgcaagaga
      481 gagcactaca tcagcttcaa caaagagatc atcaagctct gcctgttcag ccagcccagc
      541 ggcgtgcaca gcaagtacaa cctgatcgtg aacaccctga aagaggaaaa gtgggaagag
      601 aagtacaaga tcctcaaaga gaacaccttc aagatcctga agtactttta caccttcgtg
      661 aacaagaacg agctggtcat catcttttgc tttaacaagc tgatcaacaa ggccccttac
      721 gagtgccacc ggtccattaa caaggtggac ggcaagggca acgaggaaca gagagtgcgg
      781 atcaactaca accacatcga ccctctgagc ctggtggact acaccagcaa taccttcgac
      841 ttcctgggca ccagctacct gctgatctgc ggcatcaacg agaaccggaa gctgagcccc
      901 ggcagccacg tgttcatcct gtgccgggcc agcaaggaca gcggcatcac ctggggcctg
      961 accttcagct tctactaccc tcacgccaag agcagcgtgc tgtactccaa ggtggtgccc
     1021 aagatcagct acaacgagat cggcttcctg ttcttcagcc ccgacgtgtc catcaacaag
     1081 tacatcaagt gcacctaccg cgagggcaac cacttcgact gcgacgacat ccacttcatc
     1141 gacgacaagt acctgctgga catcaccaaa gtgcggggct actacctgac catcctgagc
     1201 aacaagaagg acaagaaaga aaaagtgaag ctgtggtaca tctacaacag cgtgatcagc
     1261 gagagcatgc ggaacaacgc catcagcagc ggcaatgagt acagagaggg caatctgttc
     1321 ctgctgaacg acgccacagt gctgtacaac tacctgaacg agaaggactc ctacctgtac
     1381 ctgatcaagc acaagggcag cgtgaagcac tgcaccctgc tgtatctgaa gaaagagtac
     1441 gccaaccccg gcttcgtgaa agagattaag agaaaccacg ccaccaccct gaccaaggac
     1501 aacagcaaca actaccgcta cgtgtgcaac atcaactacg acgacctgag catcaacctc
     1561 aacgaccggt acttcatcgt gtccgtgtac aacggcgtga aaaccgacat ccggtcctgc
     1621 ttctacttta gctacgacaa catcctcgag cccgtgaaca ttatcaacaa gatcgtgaag
     1681 gtctacgagt actccaacta cgccatctac gccttccaca ttaacaaaga cctcgagagc
     1741 tacttcctgt ttaacaagaa aatggaatgc ttcctcggcg acaacttcta catctacctc
     1801 aacatcaatt tcatcaacac ctacaagctg gacaactcct tcgacaacaa caatgagaag
     1861 gtggtcaacc tgtaccccaa caacctcatc tactacaagc tccccaacat taactacaag
     1921 aataccttcc tgtccaacgt gcacttccct ccctacacca aatacgtgca gctcaacgac
     1981 aaagagtaca tctttaagct gcccagctac atcgaagagg acatccacac cgagctgcac
     2041 tttctccaga aagtcaacaa gttctacaag aagaaaacca tcttccacaa gggcggccac
     2101 aacaacgacc tggtgctggg catcgacttc agcaaaaaga ccaacatctg cagctacggc
     2161 tacagcaaga ccagctgcga gtacacacac atcgacatca acaaaatcaa tatcaaggtg
     2221 cccatgaacg acgagatcac ctccgacatc accctggcca tcatctgccc cgtgtccaag
     2281 aaaaaccaga acgtgtgctt ccaccacacc tacgacaatg gcaagaaggt gctgatccgg
     2341 gaccacctgt acgaccggaa cggctacctg aatgtgtacc ctaagatcta tatctacacc
     2401 cctaagctga accagatcta cgaggaatcc aagctcgtga tccggaaaga gttcatcgag
     2461 atcttcaaga acaacaagtg caattacaag aacgtgatcc tgtgcaagtg ctacgccaac
     2521 aacaaatatt acgatatcac cttcaatctg atcggcgcgc cgtcgacctc catcacggcc
     2581 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
     2641 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
     2701 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
     2761 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
     2821 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
     2881 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
     2941 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
     3001 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
     3061 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc
     3121 ggcggctccg gaggcggtac cggtggcggc gacctagccc cacagatgct tcgagaactc
     3181 caggagacta atgcggcgct gcaagacgtg agagagctct tgcgacagca ggtcaaggag
     3241 atcaccttcc tgaagaatac ggtgatggaa tgtgacgcct gcggtctaga cagaaacctg
     3301 cctccactag cacctctggg cccacaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3361 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3421 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3481 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3541 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3601 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3661 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3721 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3781 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3841 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3901 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3961 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4021 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4081 gctgagatag gtgcctcact gattaagcat tggggccctg actacaaaga ccatgacggt
     4141 gattataaag atcatgacat cgattacaag gatgacgatg acaagggccc tcaccaccac
     4201 catcaccact aattaattaa ggatcccccg acctcgacct ctggctaata aaggaaattt
     4261 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     4321 agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc gatccccgcc
     4381 ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta
     4441 atcccttcag ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg
     4501 ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca
     4561 catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa
     4621 cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca
     4681 tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc
     4741 ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc
     4801 cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga
     4861 ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt
     4921 tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat
     4981 ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct
     5041 gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt
     5101 cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga
     5161 aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca
     5221 ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg
     5281 aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa
     5341 gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat
     5401 atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg
     5461 ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg
     5521 gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa
     5581 agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca
     5641 cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc
     5701 cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata
     5761 cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa
     5821 attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg
     5881 tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg
     5941 atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     6001 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     6061 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6121 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg
     6181 gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag
     6241 catatgctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     6301 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     6361 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     6421 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     6481 catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc
     6541 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6961 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7261 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8461 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8521 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8581 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8641 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8701 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8761 ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat cgcagatccg
     8821 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
     8881 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     8941 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     9001 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     9061 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     9181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     9241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     9301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     9361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     9421 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     9541 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9601 agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct gcgagggcca
     9661 gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact cttggatcgg
     9721 aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag tccgcatcga
     9781 ccggatcgga aaacct
//
